Abstract
Rhodinosyl transferase geneIndGT4, governing the conversion of the disaccharide oligoketide (‘polyketide’) landomycin D into a trisaccharide derivative landomycin E, was deleted inStreptomyces globisporus 1912 genome. Possible resistance mechanisms that protect the resulting landomycin D-producing mutant strainS. globisporus LD3 against the toxic action of landomycins were determined.
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Bierman M., Logan R., O’Brien K., Seno E., Rao R., Schoner B.: Plasmid cloning vectors for the conjugal transfer of DNA fromE. coli toStreptomyces spp.Gene 116, 43–49 (1992).
Depenbrock H., Bornschlegl S., Peter R., Rohr J., Schmid P., Schweighart T., Block T., Rastetter J., Hanauske A.R.: Assessment of antitumor activity of landomycin A (NSC 639 9187-A).Ann.Hematol. 73, A80/316 (1996).
Fedorenko V., Basiliya L., Pankevych K., Dubitska L., Ostash B., Luzhetskyy A., Gromyko O., Kruegel H.: Genetic control of antitumor antibiotics biosynthesis in actinomycetes.Bull.Inst.Agr.Microbiol. 2, 27–31 (2000).
Fernández-Lozano M.J., Remsing L.L., Quirós L.M., Braňa A.F., Fernandez E., Sánchez C., Méndez C., Rohr J., Salas J.A.: Characterization of two polyketide methyltransferases involved in the biosynthesis of the antitumor drug mithramycin byStreptomyces argillaceus.J.Biol.Chem. 275, 3065–3074 (2000).
Henkel T., Rohr J., Beale J., Schwenen L.: Landomycins, new angucycline antibiotics fromStreptomyces sp. Structural studies on landomycins A-D.J.Antibiot. 43, 492–503 (1990).
Kieser H.M., Bibb M.J., Buttner M.J., Chater K.F., Hopwood D.A.:Practical StreptomycesGenetics. John Innes Foundation, Norwich (UK) 2000.
Lomovskaya N., Doi-Katayama Y., Filippini S., Nastro C., Fonstein L., Gallo M.M., Colombo L., Hutchinson C.R.: TheStreptomyces peucetius dpsY anddurX genes govern early and late steps of daunorubicin and doxorubicin biosynthesis.J.Bacteriol. 180, 2379–2386 (1998).
Luzhetskyy A.M., Ostash B.O., Fedorenko V.O.: Intergeneric conjugationEscherichia coli-Streptomyces globisporus 1912 with using of integrative plasmid pSET152 and its derivative.Rus.J.Genet. 37, 1340–1347 (2001).
Matseliukh B.P., Konovalova T.A., Polishchuk L.V., Bambura O.I.: The sensitivity to landomycins A and E of streptomycetes, producers of polyketide antibiotics.Mikrobiol.Zhur. 60, 31–36 (1998).
Ostash B., Rebets Y., Yuskevich V., Luzhetskyy A., Tkachenko V., Fedorenko V.: Targeted disruption ofStreptomyces globisporus 1912lndF andlndL cyclase genes involved in antitumor landomycin E biosynthesis.Folia Microbiol. 48, 484–488 (2003).
Ostash B., Rix U., Remsing-Rix L., Liu T., Lombo F., Luzhetskyy A., Gromyko O., Wang C., Brana A., Mendez C., Salas J.A., Fedorenko V., Rohr J.: Generation of new landomycins by combinatorial biosynthetic manipulation of thelndGT4 gene of the landomycin E cluster inS. globisporus.Chem.Biol. 11, 547–555 (2004).
Panchuk R., Korynevska A., Ostash B., Osyp Y., Fedorenko V., Stoika R.: Study of mechanisms of landomycin E action on mammalian cells.Visn.Lviv Univ.Ser.Biol. 35, 54–59 (2004).
Quiros M., Aguirrezabalaga I., Olano C., Mendez C., Salas J.A.: Two glycosyltransferases and a glycosidase are involved in oleandomycin modification during its biosynthesis byStreptomyces antibioticus.Mol.Microbiol. 28, 1177–1185 (1998).
Rebets Y., Ostash B., Luzhetskyy A., Hoffmeister D., Brana A., Mendez C., Salas J.A., Bechthold A., Fedorenko V.: Production of landomycins in strainsStreptomyces globisporus 1912 andS. cyanogernus S136 is regulated by genes encoding putative transcriptional activators.FEMS Microbiol.Lett. 222, 149–153 (2003a).
Rebets Y., Ostash B., Gromyko O., Mik U., Basiliya L., Fedorenko V.: Resistance of recombinantStreptomyces albus CEST114 andStreptomyces globisporus strains to landomycin E.Visn.Lviv Univ.Ser.Biol. 33, 47–54 (2003b).
Rodriguez L., Aguirrezabalaga I., Allende N., Brańa F., Mendez C., Salas J.A.: Engineering deoxysugar biosynthetic pathways from antibiotic-producing microorganisms. A tool to produce novel glycosylated bioactive compounds.Chem. Biol. 9, 721–729 (2002).
Sambrook J., Fristch E.F., Maniatis T.:Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989.
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This work was partially supported byInternational Association — European Community, European Union’s Member States (INTAS) grant YS 00-208 to the first author andWest-Ukrainian Bio-Medical Research Center grants to the first two authors. Research in Dr. V. Fedorenko’s laboratory was supported by grant BG117b of theMinistry of Science and Education of Ukraine. Prof. J.A. Salas (Universidad de Oviedo, Spain) is acknowledged for providing us with access to HPLC and MALDI-TOF facilities in his laboratory.
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Ostash, B., Kozarevska, I. & Fedorenko, V. Generation of landomycin D-producing strainStreptomyces globisporus LD3. Folia Microbiol 50, 19–23 (2005). https://doi.org/10.1007/BF02931289
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DOI: https://doi.org/10.1007/BF02931289