Skip to main content
Log in

Thein vitro cytotoxic effect of Bilirubin on human lymphocytes and granulocytes

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Using anin vitro eytotoxicity assay (51Cr-release), it was shown that bilirubin exerts a cytotoxic effect on both adult and newborn human lymphocytes after a 1-d incubation of cells with bilirubin. The effect of bilirubin on human granulocytes was less pronounced; the association of the cytotoxic effect with a functional immunological perturbation of these cells is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ballowitz L.: Hyperbilirubinämie bei Früh- und Neugeborenen.Pädiat. Prax.13, 213 (1974).

    Google Scholar 

  • Brodersen R.: Binding of bilirubin to albumin and tissues, p. 144 inPhysiological and Biochemical Basis for Perinatal Medicíne (Samuel Z. Levine Conf., lst Intern. Meeting, Paris 1979). Karger, Basel 1981.

    Google Scholar 

  • Cowger M.L.: Mechanisms of bilirubin toxicity on tissue culture cells: faetors that affect toxicity, reversibility by albumin and comparison with other respiratory poisons and surfactants.Biochem. Med.5, 1 (1971).

    Article  PubMed  CAS  Google Scholar 

  • De Sanctis C., Malandra C., Zanetti P., Pabris C., Ponzone A.: Neonatal hyperbilirubinaemia and response of lymphocytes to phytohaemagglutinin.Minerva Paediat.20, 2011 (1968).

    Google Scholar 

  • Diaz del Castillo E.: Neonatal hyperbilirubinaemia. Its relation to the immunological response capacity of newborn infants.Gac. Med. Mex.105, 185 (1973).

    Google Scholar 

  • Leipb S., Mohrig W., Meisel P.: Bilirubin-induced cytomorphological changes in guinea-pig leucocytes.Biomed. Biochim. Acta5, 503 (1983).

    Google Scholar 

  • Maisels M.F.: Bilirubin. On understanding and influencing its metabolism in the newborn infant.Pediatr. Clin. N. Amer.19, 447 (1972).

    CAS  Google Scholar 

  • Metze H.: Über die Toxizität des Bilirubins.Klin. Pädiat.189, 393 (1977).

    CAS  Google Scholar 

  • Miler I.: The influence of hyperbilirubinaemia on the immunity of newborn and the immuno-suppressive effect of bilirubin inin vivo andin vitro experimental models (in Czech).Čs. Pediat.35, 351 (1980).

    CAS  Google Scholar 

  • Miler I., Vondráček J., Hromádková L.: The bactericidal activity of sera of healthy neonates and newborns with hyperbilirubinaemia toEscherichia coli.Folia Microbiol.24, 143 (1979).

    Article  CAS  Google Scholar 

  • Miler I., Vondráček J., Hromádková L.: Bilirubin inhibits the chemotactic activity of human polymorphonuclear leukocytesin vitro.Folia Microbiol.26, 413 (1981).

    CAS  Google Scholar 

  • Nejedlá Z.: The development of immunological factors in infants with hyperbilirubinaemia.Pediatrics45, 102 (1970).

    PubMed  Google Scholar 

  • Odell G.B.:Neonatal Hyperbilirubinaemia. Grune and Stratton, New York-London -Toronto - Sydney-San Francisco 1980.

    Google Scholar 

  • Poláček K.:Physiology and Pathology of the Newborn (in Czech). Avicenum, Prague 1981.

    Google Scholar 

  • Bola-Plesczynski M., Hensen S.A., Vincent M.M., Bellanti J.A.: Inhibitory effects of bilirubin on cellular immune response in man.J. Pediat.86, 690 (1975).

    Article  Google Scholar 

  • Ross L., Fremland H.: Myocardial hyperbilirubinaemia in an premature infant.Amer. J. Dis. Child.115, 358 (1968).

    PubMed  CAS  Google Scholar 

  • Rubaltelli F.F., Granati B., Fortunato A., Piovesan A., Casara G., Colleselli P., Semenzato G.: Inhibitory effect of bilirubin and photobilirubin on neonatal and adult T lymphocytes and granulocytes.Biol. Neonate42, 152 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Rubaltelli F.F., Piovesan A.L., Semenzato G., Barbato A., Ongaro G.: Immune competence assessment in hyperbilirubinemic newborns before and after phototherapy.Helv. Paediat. Acta32, 129 (1977).

    PubMed  CAS  Google Scholar 

  • Sanderson A.R.: Quantitative titration, kinetic behaviour and inhibition of cytotoxic mouse antisera.Immunology9, 287 (1965).

    PubMed  CAS  Google Scholar 

  • Suvansri U.W., Cheung W.H., Sawitsky A.: The effect of bilirubin on the human platelet.J. Pediat.74, 240 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Šíma P., Malá I., Mileb I., Hodr R., Truxová E.: The suppressive effect of continuous infusion of bilirubin on theimmune response in mice.Folia Microbiol.25, 483 (1980).

    Article  Google Scholar 

  • Švejcar J., Miler I., Pekárek J.: Effect of bilirubin on anin vitro correlate of cell-mediated immunity - the migration inhibition test.J. Lab. Clin. Immunol.13, 145 (1984).

    Google Scholar 

  • Thong Y.H., Ferrante A.: Adverse effect of bilirubin on the chemotactic responsiveness of human neutrophils.IRCS Med. Sci.5, 483 (1977).

    CAS  Google Scholar 

  • Thong Y.H., Ness D., Ferrante A.: Effect of bilirubin on thefungicidal capacity of human neutrophils.Sabouraudia17, 125 (1979).

    PubMed  CAS  Google Scholar 

  • Thong Y.H., Rencis V.: Bilirubin inhibits hexose-monophosphate shunt activity of phagocytosing neutrophils.Acta Paediatr. Scand.66, 757 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Wigzell H.: Quantitative titration of mouse H-2 antibodies using51Cr labelled target cells.Transplantation3, 423 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Xanthou M., Mandyla-Sfagou E., Campbell A.C., Waller C.A., Economou-Mavrou C., Matsaniotis N.: Lymphocyte subpopulations and their function in the blood of neonates, p. 139 inIntensive Care in the Newborn. Masson Publ. Inc., New York 1976.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miler, I., Indrová, M., Bubeník, J. et al. Thein vitro cytotoxic effect of Bilirubin on human lymphocytes and granulocytes. Folia Microbiol 30, 272–276 (1985). https://doi.org/10.1007/BF02923520

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02923520

Keywords

Navigation