Abstract
A method is described for isolating DNA of high molecular mass (Mr) from blackcurrant and other soft-fruit species. Following a hexacethylytimethyl ammonium bromide (CTAB)-based extraction procedure, samples are treated with a glycosidic hydrolase mixture and RNase, and then purified. The suitability of this DNA for Southern analysis and genomic-library construction is demonstrated.
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Mary, W., Davies, H.V., Brennan, R.M. et al. The isolation of genomic DNA from blackcurrant (ribes nigrum L.). Mol Biotechnol 9, 243–246 (1998). https://doi.org/10.1007/BF02915797
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DOI: https://doi.org/10.1007/BF02915797