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Effect of calcium on water-stress-induced biochemical changes and yield of field-grown rice

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Biologia Plantarum

Abstract

Three different treatments by calcium (102M), namely seed treatment, foliar spraying and their combination were applied on field-grown rice (Oryza sativa L. cv. Ratna) under both water stressed and non-stressed conditions in the course of plant development. The relative water content and leaf water potential decreased with increase in age of stressed and non-stressed plants. Pretreatment of seeds with Ca improved the water status of the plants most prominently at the vegetative stage but the effect gradually faded away with plant development. The foliar spraying by Ca was more effective in improving the water status of the plants at the reproductive stage. The combined Ca treatment significantly improved water status of the plants both at the vegetative and reproductive stages. The contents of chlorophyll and protein decreased and the activities of protease and RNase increased in the course of plant development in both non-stressed and even more in stressed plants. Ca treatments of seeds or plants or their combination inhibited the decline in chlorophyll and protein contents and the rising trends of protease and RNase activities, the combined treatment being most effective. During plant development free proline content increased significantly more in water stressed plants. In non-stressed plants there was a marked increase in the free proline content at the mature fruit stage. Ca treatment inhibited the rise of free proline in stressed plants. A significant reduction in yield components and yield of the crop in water stressed plants was increased by Ca treatment.

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References

  • Arnon, D. I.: Copper enzyme in isolated chloroplast. Polyphenoloxidase inBeta vulgaris. - Plant Physiol.24: 1–15, 1949.

    PubMed  CAS  Google Scholar 

  • Bates, L. S., Waldenz, R. P., Teaee, I. D.: Rapid determination of free proline for water stress studies. - Plant Soil39: 205–207, 1973.

    Article  CAS  Google Scholar 

  • Biswas, A. K., Choudhuri, M. A.: Mechanism of monocarpic senescence in rice. - Plant Physiol.65 : 340–345, 1980.

    PubMed  CAS  Google Scholar 

  • Chokwang, Y. I., Todd, W.: Changes in ribonuclease activity of wheat plants during water stress. - Physiol. Plant.46: 13–18, 1979.

    Article  Google Scholar 

  • Dwivedi, S., Kar, M., Mishra, D.: Biochemical changes in excised leaves ofOryza sativa subjected to water stress. - Physiol. Plant.45: 35–40, 1979.

    Article  CAS  Google Scholar 

  • Fick, N. G., Qualset, C. O.: Genetic control of endosperm amylase activity and gibberellin responses in standard height and short statured wheat. - Proc. nat. Acad. Sci. USA72: 892–895, 1975.

    Article  PubMed  CAS  Google Scholar 

  • Fisher, R. A., Yates, F.: Statistical Tables for Biological, Agricultural and Medical Research. 6th Ed. -Longman Gr., London 1974.

    Google Scholar 

  • Gates, C. T.: Water deficits and growth of herbaceous plants. - In:Kozlowski, T. T. (ed.): Water Deficits and Plant Growth. Vol. 2. Pp. 135–190. Academic Press, New York-London 1968.

    Google Scholar 

  • Hanson, A. D., Nelsen, C. E., Everson, E. H.: Evaluation of proline accumulation as an index of drought resistance using two contrasting barley cultivars. - Crop Sci.17: 720–726, 1977.

    CAS  Google Scholar 

  • Hsiao, T. C.: Plant response to water stress. - Annu. Rev. Plant Physiol.24: 519–570, 1973.

    Article  CAS  Google Scholar 

  • Hsiao, T. C., Acevedo, E., Fereres, E.: Stress metabolism-water stress, growth and osmotic adjustment. - Phil. Trans. roy. Soc. Lond. B273: 479–500, 1976.

    Article  Google Scholar 

  • Knipling, E. B.: Measurement of leaf water potential by the dye method. - Ecology48: 1038 to 1041, 1967.

    Article  Google Scholar 

  • Levitt, J.: Responses of Plants to Environmental Stresses. - Academic Press, New York-London 1972.

    Google Scholar 

  • Livne, A., Vaadia, Y.: Water deficits and hormone relations. - In:Kozlowski, T. T. (ed.): Water Deficits and Plant Growth. Vol. 3. Pp. 255–276. Academic Press, New York-London 1972.

    Google Scholar 

  • Lowry, O., Rosebrough, N. J., Fare, A. L., Randall, R. J.: Protein measurement with Folin-phenol reagent. - J. biol. Chem.193: 265–275, 1951.

    PubMed  CAS  Google Scholar 

  • Mali, P. C., Mehta, S. L.: Effect of drought on enzymes and free proline in rice varieties. - Phytochemistry16: 1355–1358, 1977.

    Article  CAS  Google Scholar 

  • Mukherjee, S. P., Choudhuri, M. A.: Effect of water stress on some oxidative enzymes and senescence inVigna seedlings. - Physiol. Plant.51: 37–42, 1981.

    Article  Google Scholar 

  • Parija, P., Pillay, K. P.: Effect of presowing treatment on the drought resistance in rice. - Proc. nat. Acad. Sci. India15B: 6–14, 1945.

    Google Scholar 

  • Poovaiah, B. W., Leopold, A. C.: Deferral of leaf senescence with calcium. - Plant Physiol.52: 235–239, 1973.

    Google Scholar 

  • Ray, S., Choudhuri, M. A.: Mobilization of metabolites from leaves to grains as the cause of monocarpie senescence in rice plant. - Plant Physiol.68: 1345–1348, 1981.

    PubMed  CAS  Google Scholar 

  • Salim, M. H., Todd, G. W.: Seed soaking as a presowing, drought hardening treatment in wheat and barley seeds. -Agron. J.60: 179–182, 1968.

    CAS  Google Scholar 

  • Snell, F. D., Snell, C. T.: Colorimetric Methods of Analysis. Vol. IV. - Van Nostrand Reinhold Co., New York 1971.

    Google Scholar 

  • Stewart, C. R., Morris, C. J., Thompson, J. F.: Changes in amino acid content of excised leaves during incubation II. Roles of sugar in the accumulations of proline in wilted leaves. - Plant Physiol.41: 1585–1590, 1966.

    PubMed  CAS  Google Scholar 

  • Tully, R. E., Hanson, A. D., Nelsen, C. E.: Proline accumulation in water stress barley leaves in relation to translocation and nitrogen budget. - Plant Physiol.63: 518–523, 1979.

    Article  PubMed  CAS  Google Scholar 

  • Weatherley, P. E.: Studies in the water relations of the cotton plant I. The field measurement of water deficit in leaves. -New Phytol.49: 81–97, 1950.

    Article  Google Scholar 

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Nayek, B., Biswas, A.K. & Choudhuri, M.A. Effect of calcium on water-stress-induced biochemical changes and yield of field-grown rice. Biol Plant 25, 117–123 (1983). https://doi.org/10.1007/BF02902121

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