Skip to main content
Log in

The effect ofGinkgo biloba on the activity of catalase and lipid peroxidation in experimental strangulation ileus

  • Brief Definitive Report
  • Published:
International Journal of Clinical and Laboratory Research

Abstract

This study was designed to assess the therapeutic effect ofGinkgo biloba extract (EGb 761) in experimental strnagulation ileus. Rats were divided into control (n=7), placebo (n=11), and EGb-treated (n=11) groups. No surgical procedure was carried out on the control group. Strangulation ileus was produced in the placebo and EGb groups for 2.5 h. At the end of this period, 100 mg/kg EGb in 1 ml of saline was injected intraperitoneally to the EGbtreated group. In the placebo group, animals received an equivalent amount of saline intraperitoneally; 24 h later, repeat laparotomies were performed to take blood and intestinal tissue samples. The EGb treatment decreased tissue malondialdehyde levels and increased catalase activities compared with the placebo group (P<0.05 for both). Serum creatine kinase and phosphorus levels were also determined in all groups. In the placebo group these were significantly higher than in the control group (P<0.01 andP<0.05, respectively). In the EGb group these were not different from controls and the increase in creatine kinase activity in the EGb group was not as high as in the placebo group (P<0.05). Our results suggest that EGb could be preventive against the effects of strangulation ileus in a rat model.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Schwartz SI. Manifestations of gastrointestinal disease. In: Schwartz SI, ed. Principles of surgery. New York: McGraw-Hill; 1989: 1061–1082.

    Google Scholar 

  2. Oshima A, Kitajima M, Sakai N, Ando N. Does glucagon improve the viability of ischemic intestine. J Surg Res 1990; 49:524.

    Article  PubMed  CAS  Google Scholar 

  3. Parks DA, Granger DN. Contributions of ischemia and reperfusion to mucosal lesion formation. Am J Physiol 1986; 250: G749.

  4. Zimmerman BJ, Granger DN. Reperfusion injury. Surg Clin North Am 1992; 72:65.

    PubMed  CAS  Google Scholar 

  5. Schoenberg MH, Beger HG. Oxygen radicals in intestinal ischemia and reperfusion. Chem Biol Interact 1990; 76:141.

    Article  PubMed  CAS  Google Scholar 

  6. Filep J, Herman F, Braquet P, Mozes T. Increased levels of platelet-activating factor in blood following intestinal ischemia in the dog. Biochem Biophys Res Commun 1989; 158:353.

    Article  PubMed  CAS  Google Scholar 

  7. Patei A, Kaleya RN, Sammartana RJ. Pathophysiology of mesenteric ischemia. Surg Clin North Am 1992; 72:31.

    Google Scholar 

  8. Kubes P, Suzuki M, Granger DN. Platelet-activating factor induced microvascular dysfunction: role of adherent leukocytes. Am J Physiol 1990; 258:G158.

    Google Scholar 

  9. Grisham BM, Hernandez AL, Granger DN. Xanthine oxidase and neutrophil infiltration in intestinal ischemia. Am J Physiol 1986; 251:G567.

    Google Scholar 

  10. Droy Lefaix MT, Drout Y, Geraud G, Hosford D, Braquet P. Superoxide dismutase and the PAF antagonist (BN 52021) reduce small intestinal damage induced by ischemia-reperfusion. Free Radic Res Commun 1991; 2:725.

    Article  Google Scholar 

  11. Drieu K. Preparation and definition ofGinkgo biloba extract. In: Fünfgeld EW, ed. Rökan (Ginkgo biloba). Recent results in pharmacology and clinic. Berlin Heidelberg New York: Springer; 1988:32–36.

    Google Scholar 

  12. Pincemail J, Dupuis M, Nasr C, Hans P, Haag-Berrurier M, Anton R, Deby C. Superoxide anion scavenging effect and superoxide dismutase activity ofGinkgo biloba extract. Experientia 1989; 45:708.

    Article  PubMed  CAS  Google Scholar 

  13. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in antimal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95:351.

    Article  PubMed  CAS  Google Scholar 

  14. Beutler E. Red cell metabolism. A manual of biochemical methods. New York: Grune and Stratton, 1973.

    Google Scholar 

  15. Otamiri T, Lindahl M, Tagesson C. Phospholipase A2 inhibition prevents mucosal damage associated with small intestinal ischemia in rats. Gut 1988; 29:489.

    Article  PubMed  CAS  Google Scholar 

  16. Kubes P, Ibbotson G, Russel J, Wallace JL, Granger DN. Role of platelet activating factor in ischemia/reperfusion induced leukocyte adherence. Am J Physiol 1990; 259:G300.

    Google Scholar 

  17. Şahin A, Çolak Ö, Alataş Ö, Bayçu C, Kasap HA, Yaşar B, Karahüseyinoğlu E, Kiper H. The effect ofGinkgo biloba extract in experimental strangulation ileus in a rat model. Turk J Gastroenterol 1995; 6:162.

    Google Scholar 

  18. Graeber GM, Cafferty PJ. Changes in peripheral serum creatine phosphokinase and lactate dehydrogenase in acute experimental colonic infarction. Ann Surg 1982; 194:708.

    Article  Google Scholar 

  19. Kurland B, Brandt LJ, Delany HM. Diagnostic test for intestinal ischemia. Surg Clin North Am 1992; 72:85.

    PubMed  CAS  Google Scholar 

  20. De Toma G, Marazano D, Salvatore P. Enzymatic and metabolic changes in the peripheral serum after superior mesenteric artery ligation in dogs. Ital J Surg Sci 1983; 13:269.

    PubMed  Google Scholar 

  21. Jamieson W, Marchuk S, Rowson J. The early diagnosis of massive acute intestinal ischemia. Br J Surg 1982; 69:S52.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Çolak, Ö., Şahin, A., Alataş, Ö. et al. The effect ofGinkgo biloba on the activity of catalase and lipid peroxidation in experimental strangulation ileus. Int J Clin Lab Res 28, 69–71 (1998). https://doi.org/10.1007/BF02874083

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02874083

Key words

Navigation