Abstract
The most commonly encountered difficulties for the quantitative measurement of zinc in biological samples are the limited sample amount, total and effective digestion of connective and fatty residues, and low zinc concentrations. These problems often lead to the determination of lower zinc values than actually present, so that the sample preparation, digestion, and analytical procedure deserve careful attention.
In this short communication, a new method for microwave tissue disintegration is described. The authors have obtained consistent and reproducible results with tissue samples of 0.5 g or less.
References
R. J. P. Williams, Zinc: what is its role in biology?Endeavor 8, 65–70 (1984).
A. S. Prasad, A. O. Cavdar, G. J. Brewer, and P. J. Aggett, inZinc Deficiency in Human Subjects, Alan R. Liss, Inc., ed. New York (1983).
C. F. Mills,Zinc in Human Biology, Springer-Verlag, New York (1989).
P. J. Fraker, M. E. Gershwin, R. A. Good and A. Prasad, Interrelationships between zinc and immune function,Fed. Proc. 45, 1474–1479 (1986).
J. A. Coto, E. H. Hadden, M. Sauro, N. Zorn, and J. W. Hadden, Interleukin 1 regulates secretion of zinc-thymulin by human thymic epithelial cells and its action on T-lymphocyte proliferation and nuclear protein kinase C,Proc. Natl. Acad. Sci. USA89, 7752–7756 (1992).
N. W. Tietz,Clinical Guide to Laboratory Tests, W. B. Saunders, Philadelphia (1983).
K. M. Hambridge, C. E. Casey, and N. F. Krebs, Zinc. inTrace Elements in Human and Animal Nutrition, W. Mertz, ed., Academic, Orlando, FL, pp. 1–137 (1986).
J. Smeyers-Verbeke, C. May, P. Drochmans, and D. L. Massart, The determination of Cu, Zn, and Mn in subcellular rat liver fractions,Ann. Biochem. 83, 746–753 (1977).
M. S. Clegg, C. L. Keen, B. Lonnerdal, and L. S. Hurley, Influence of ashing techniques on the analysis of trace elements in animal tissue,Biol. Trace Element Res. 3, 107–115 (1981).
A. T. Zander, Atomic emission sources for solution spectrochemistryAnal. Chem. 58, 1139–1149 (1986).
C. Mascia, W. Capone, M. Melis, and D. Valenti, Determination of heavy metals in animal tissues,Il Farmaco 45, 777–781 (1990).
G. Leblondel, Y. Mauras, and P. Allain, Tissue determination of some elements in rats,Biol. Trace Element Res. 10, 327–333 (1986).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Verbanac, D., Milin, C., Domitrovic, R. et al. Determination of standard zinc values in the intact tissues of mice by ICP spectrometry. Biol Trace Elem Res 57, 91–96 (1997). https://doi.org/10.1007/BF02803873
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02803873