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The effect of melatonin on lipid peroxidation during radiotherapy in female rats

Die wirkung von melatoningabe auf die lipidperoxidation während der strahlentherapie in weiblichen ratten

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Abstract

Background

Because radiotherapy is one of the causes of primary or secondary ovarian failure, protection of ovarian functions in the patients receiving total body or pelvic radiotherapy is of importance. In this study, we investigated the role of melatonin in the oxidative damage in both whole body and ovaries, which is caused by radiotherapy.

Materials and Methods

Eighteen female rats were divided into 3 groups, each of which consisted of 6 rats. First group was control group receiving no treatment, second group received total body radiotherapy (RT) by 2 × 360 cGy only and third group received radiotherapy plus melatonin. Malondialdehyde (MDA) levels in both blood and ovarian tissue were detected as the indicator of free radical (FR) damage. Levels of erythrocyte superoxide dismutase (SOD) and glutathion peroxidase (GPX) in blood were measured as the indicators of antioxidant level.

Results

Radiotherapy caused a significant increase in the levels of MDA in blood and ovarian tissue (p < 0.001). However, MDA levels decreased in the radiotherapy plus melatonin group (p < 0.05). SOD and GPX levels decreased insignificantly in the radiotherapy only group while they increased in the radiotherapy plus melatonin group significantly (p < 0.01 and p < 0.05, respectively).

Conclusion

Melatonin, in rats, reduced the level of MDA, which is elevated by radiotherapy and increased the levels of SOD and GPX, which are involved in the antioxidant system.

Zusammenfassung

Ziel

Da die Strahlentherapie eine der Ursachen der primären bzw. sekundären Ovarialinsuffienz ist, ist es wichtig, die ovariellen Funktionen der Patienten, die einer Beckenoder Gesamtkörperstrahlentherapie unterzogen werden, zu schützen. In dieser Studie untersuchten wir daher, ob Melatonin in der Lage ist, die oxidative Schädigung sowohl des Gesamtkörpers als auch der Eierstocke durch Strahlentherapie zu verringern.

Material und Methode

18 weibliche Ratten wurden in drei Gruppen unterteilt, von denen jede aus sechs Ratten bestand. Die erste Gruppe war die unbehandelte Kontrollgruppe, die zweite Gruppe empfing nur eine Strahlentherapie von 2mal 360 cGy, und die dritte Gruppe wurde strahlentherapiert und erhielt Melatonin (Radiotherapie + Melatonin). Der Gehalt an Malondialdehyd (MDA) im Blut und ovariellen Gewebe wurde als Indikator für die Schädigung durch freie Radikale bestimmt. Die Bestimmung von Superoxiddismutase (SOD) und von Glutathionperoxidase (GPX) diente als Indikator des antioxidativen Status.

Ergebnisse

Strahlentherapie verursachte eine bedeutende Zunahme des Gehalts von MDA im Blut und im ovariellen Gewebe (p < 0,001). In der Gruppe der Tiere, die Strahlentherapie und Melatonin erhielten, waren die MDA-Werte signifikant erniedrigt (p < 0,05), SOD und GPX waren in der Gruppe 2 (Radiotherapie) unverandert gegenüber der Kontrolle und erhöhten sich signifikant in der Gruppe 3 (Radiotherapie + Melatonin), (p < 0,01 für SOD und < 0,05 für GPX).

Schlußfolgerungen

Melatonin verringert bei Ratten den Gehalt an MDA nach Strahlentherapie und erhöht zudem den Gehalt an SOD und GPX, welche antioxidativ wirken.

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Authors and Affiliations

  1. Gynecology and Obstetrics Department, S. Demirel University, School of Medicine, Isparta, Turkey

    Hakan Kaya & Okan Özkaya

  2. Biochemistry Department, S. Demirel University, School of Medicine, Isparta, Turkey

    Namik Delibas

  3. Biochemistry Department, Akdeniz University, School of Medicine, Antalya, Turkey

    Mustafa Serteser

  4. Biochemistry Department, Uludag University, School of Medicine, Bursa, Turkey

    Engin Ulukaya

Authors
  1. Hakan Kaya
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  2. Namik Delibas
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  3. Mustafa Serteser
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  4. Engin Ulukaya
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  5. Okan Özkaya
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We would like to acknowledge the support of Private Oncology Centre.

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Kaya, H., Delibas, N., Serteser, M. et al. The effect of melatonin on lipid peroxidation during radiotherapy in female rats. Strahlentherapie und Onkologie 175, 285–288 (1999). https://doi.org/10.1007/BF02743581

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  • DOI: https://doi.org/10.1007/BF02743581

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