Skip to main content
Log in

Analysis of cannabinoids in fiber hemp plant varieties (Cannabis sativa L.) by high-performance liquid chromatography

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

An analytical procedure was developed for the detection of neutral and acidic cannabinoids in herbal cannabis without the need of any preliminary derivatization. The method was used to assay cannabinoid content of over one hundred fiber hemp samples grown in different Italian localities and harvested at different maturation level degrees during the summer. No interferences were observed due to the vegetal matrix.

The influence of genetic factors and environmental conditions on cannabinoid content is discussed; the results may be of interest to enhance potential of fiber hemp in compliance with law enforcement purposes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. H. A. Hakim, Y. M. El Kheir, M. I. Mohamed, Fitoterapia57, 239 (1986).

    CAS  Google Scholar 

  2. E. P. M. de Meijer, H. J. van der Kamp, F. A. van Eeuwijk, Euphytica62, 187 (1992).

    Article  Google Scholar 

  3. P. S. Fetterman, E. S. Keith, C. W. Waller, O. Guerrero, N. J. Doorendos, M. W. Quimby, J. Pharm. Sci.60, 1246 (1971).

    CAS  Google Scholar 

  4. E. Small, H. D. Beckstead, Lloydia36, 144 (1973).

    CAS  Google Scholar 

  5. G. Fournier, M. R. Paris, Plant Med. Phytother.13, 116 (1979).

    CAS  Google Scholar 

  6. J. C. Turner, P. G. Mahlberg, J. Chromatogr.254, 295 (1982).

    Article  Google Scholar 

  7. C. E. Turner, M. A. Elsohly, E. G. Boeren, J. Natural Prod.43, 169 (1980).

    Article  CAS  Google Scholar 

  8. F. Höppner, U. Menge-Hartmann, Landbauforschung Völkenrode46, 55 (1996).

    Google Scholar 

  9. S. Björkman, J. Chromatogr.237, 389 (1982).

    Article  Google Scholar 

  10. J. C. Turner, P. G. Mahlberg, J. Chromatogr.283, 165 (1984).

    Article  CAS  Google Scholar 

  11. T. Veress, J. I. Szanto, L. Leisztner, J. Chromatogr.520, 339 (1990).

    Article  CAS  Google Scholar 

  12. K. A. Kovar, H. Linder, Arch. Pharm.324, 329 (1991).

    CAS  Google Scholar 

  13. J. E. Pitts, J. D. Neal, T. A. Gough, J. Pharm. Pharmacol.44, 947 (1992).

    CAS  Google Scholar 

  14. T. Lehmann, R. Brenneisen, J. Liq. Chromatogr.18, 689 (1995).

    CAS  Google Scholar 

  15. C. Rustichelli, V. Ferioli, F. Vezzalini, M. C. Rossi, G. Gamberini, Chromatographia43, 129 (1996).

    Article  CAS  Google Scholar 

  16. S. L. Kanter, M. R. Musumeci, L. E. Hollister, J. Chromatogr.171, 504 (1979).

    Article  CAS  Google Scholar 

  17. J. C. Miller, J. N. Miller, “Statistics for Analytical Chemistry”; R. A. Chalmers and M. Masson, ed., Ellis Horwood, Chichester, 1984, p. 96.

    Google Scholar 

  18. R. N. Smith, J. Chromatogr.115, 101 (1975).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rustichelli, C., Ferioli, V., Baraldi, M. et al. Analysis of cannabinoids in fiber hemp plant varieties (Cannabis sativa L.) by high-performance liquid chromatography. Chromatographia 48, 215–222 (1998). https://doi.org/10.1007/BF02467674

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02467674

Key Words

Navigation