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Concomitant protein phosphorylation and endogenous acetylcholine release induced by nicotine: Dependency on neuronal nicotinic receptors and desensitization

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Summary

1. Nicotine stimulated two Ca2+-dependent processes in rat frontal cortex synaptosomes: the phosphorylation of an 80-kDa protein band and the release of endogenous ACh.3 Both effects were mediated by neuronal nAChRs and coincided with depolarization of the synaptosomal plasma membrane induced by the drug. Changes in the state of phosphorylation of the 80-kDa band (presumed to contain synapsin I) were correlated with changes in the release of ACh as follows, from 2 to 4.

2. Blockade of predominant, nerve terminal P-type Ca2+ channels with ω-agatoxin-IVA, did not prevent nicotine from stimulating ACh release. In contrast, exposure to the toxin partially inhibited the release promoted by the depolarizing agent veratridine and attenuated protein phosphorylation induced by either nicotine or veratridine. Taken together, these data suggest that, upon nicotine stimulation, Ca2+ enters nerve terminals through two distinct pathways. The first, via Ca2+ channels, is necessary (but not sufficient) for both nicotine-induced phosphorylation and ACh release. The second, both necessary and sufficient for nicotine-induced phosphorylation and release, is the neuronal nAChR itself.

3. Preincubation of the synaptosomes with a subeffective concentration of nicotine inactivated both nicotine-induced ACh liberation and phosphorylation. This shows that diminished release is associated to decreased phosphorylation of the 80-kDa protein band, most likely as a consequence of nicotine-promoted nAChR desensitization.

4. Augmented ACh release and phosphorylation of the 80-kDa protein band were achieved by using the protein phosphatase inhibitor okadaic acid. However, okadaic acid did not summate with either nicotine or veratridine to increase ACh release further. This is probably because okadaic acid, as in other neurons, increases intracellular Ca2+ (Cholewinskiet al., 1993), thus promoting desensitization of ACh release.

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Abbreviations

DCFscein:

2′,7′-dichlorofluorescein

CDFDA:

2′,7′-dichlorofluorescin diacetate

DCFscin:

2′,7′-dichlorofluorescin

Ach:

acetylcholine

AChE:

acetylcholinesterase

α-BuTx:

α-bungarotoxin

Ca2+-free HBS:

calcium-free HEPES buffered saline

Cho:

choline oxidase

κ-BuTx:

κ-bungarotoxin

nAChRs:

nicotinic acetylcholine receptors

OKA:

okadaic acid

ω-ATX:

ω-agatoxin IVA

PEG:

polyethylene glycol

PP:

protein phosphatase

E m :

synaptosomal membrane potential

TPP+ :

tetra-[14C]phenylphosphonium ion

TTX:

tetrodotoxin

DTT:

dithiothreitol

SDS:

sodium dodecyl sulfate

PAGE:

polyacrylamide gel electrophoresis

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  1. Department of Pediatrics, Research Division of Neonatology, School of Medicine, University of California at Davis, 95616, Davis, California

    Enrique L. M. Ochoa & Sean M. O'Shea

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  1. Enrique L. M. Ochoa
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Ochoa, E.L.M., O'Shea, S.M. Concomitant protein phosphorylation and endogenous acetylcholine release induced by nicotine: Dependency on neuronal nicotinic receptors and desensitization. Cell Mol Neurobiol 14, 315–340 (1994). https://doi.org/10.1007/BF02088714

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