Abstract
Microbial modulation of myoelectric activity in small intestine was studied. Germ-free male Sprague-Dawley rats were equipped with bipolar electrodes from the duodenojejunal junction to the midpoint of small intestine. Prior to and one week after introduction of conventional intestinal microflora, 32±5% and 61±5% (mean±se), respectively, of activity fronts of the migrating myoelectric complex reached the midpoint (P<0.05), and the interval between activity fronts in proximal jejunum was reduced from 31.2±2.0 min to 17.5±0.8 min, respectively (P<0.01). The pattern of propagation was more regular after conventionalization. Slow-wave frequency in proximal jejunum was 38.5±1.2/min in germ-free rats and 43.0±0.8/min in conventional rats (P<0.01), but introduction of microflora failed to increase the frequency in germ-free rats. The frequency of spike potentials succeeding jejunal infusion of 5 ml of 12.5% glucose remained unchanged after conventionalization. Statistical analyses showed that the interval between activity fronts varied mainly within rats, whereas the propagation velocity showed statistically significant variability between rats (P<0.01), regardless of intestinal microflora. Luminal control by the resident microflora is important for physiological cycling and aboral propagation of the migrating myoelectric complex, but seems to be of no major consequence for postprandial myoelectric response.
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References
Gordon HA: Is the germfree animal normal? A review of its anomalies in young and old age.In The Germfree Animal in Research. ME Coates (ed). London, Academic Press, 1968, pp 127–148
Van Eldere J, Robben J, Caenepeel PH, Eyssen H: Influence of a cecal volume-reducing intestinal microflora on the excretion and entero-hepatic circulation of steroids and bile acids. J Steroid Biochem 29(1):33–39, 1988
Abrams GD, Bishop JE: Effect of normal microbial flora on gastrointestinal motility. Proc Soc Exp Biol 126:301–304, 1967
Caenepeel PH, Janssens J, Vantrappen G, Eyssen H, Coremans G: Interdigestive myoelectric complex in germ-free rats. Dig Dis Sci 34:1180–1184, 1989
Husebye E, Hellström PM, Midtvedt T: Introduction of conventional microbial flora to germfree rats increases the frequency of migrating myoelectric complexes. J Gastrointest Motil 4:39–45, 1992
Wingate DL: Backwards and forwards with the migrating complex. Dig Dis Sci 26:641–664, 1981
Husebye E, Skar V, Aalen OO, Osnes M: Digital ambulatory manometry of the small intestine in healthy adults: Estimates of the variation within and between individuals and statistical management of incomplete MMC periods. Dig Dis Sci 35:1057–1065, 1990
Ruckebusch M, Fioramonti J: Electrical spiking activity and propulsion in the small intestine in fed and fasted rats. Gastroenterology 68:1500–1508, 1975
Drasar BS, Shiner M, Mcleod GM: Studies on the intestinal flora. The bacterial flora of the gastrointestinal tract in healthy and achlorhydric persons. Gastroenterology 56:71–79, 1969
Luckey TD: Intestinal flora.In Conference on Nutrition in Space and Related Waste Problems. Washington, D.C., National Aeronautics and Space Administration, 1964, pp 227–242
Gustafsson BE: Lightweight stainless steel system in rearing germfree rats. Ann NY Acad Sci 78:17–28, 1959
Flatt AJ, Summers RW: Computer analysis of intestinal motor activity. Automed 7:221–236, 1987
Gustafsson BE, Midtvedt T, Norman AJ: Isolated fecal microorganisms capable of 7α-dehydroxylating bile acids. J Exp Med 123:413–432, 1966
Midtvedt T, Carlstedt-Duke B, Hqverstad T, Midtvedt AC, Norin KE, Saxerholt H: Establishment of biochemically active intestinal ecosystem in ex-germfree rats. Appl Environ Microbiol 53(12):2866–2871, 1987
Miller MS, Galligan JJ, Burks TF: Accurate measurements of intestinal transit in the rat. J Pharmacol Methods 6:211–217, 1981
Armitage P, Berry G: Statistical Methods in Medical Research, 2nd ed. Oxford, Blackwell, 1987, pp 196–200
Grivel ML, Ruckebusch Y: The propagation of segmental contractions along the small intestine. J Physiol 227:611–625, 1972
Szurszewski JH: A migrating electric complex of the canine small intestine. Am J Physiol 217(6):1759–1763, 1969
Kellow JE, Borody S, Phillips S, Tucker RL, Haddad AC: Human interdigestive motility: Variations in patterns from esophagus to colon. Gastroenterology 91:386–395, 1986
Smith WH: Observations on the flora of the alimentary tract of animals and factors affecting its composition. J Pathol Bacteriol 89:95–124, 1965
Gorbach LS, Plaut AG, Nahas L, Weinstein L: Studies of intestinal microflora. Microorganisms of the small intestine and their relations to oral and fecal flora. Gastroenterology 53(6):856–867, 1967
Dixon JMS: The fate of bacteria in the small intestine. J Pathol Bacteriol 79:131–140, 1960
Scott LD: Influence of the interdigestive myoelectric complex on enteric flora in the rat. Gastroenterology 82:737–745, 1982
Al Saffar A, Hellström PM, Nylander G: Correlation between peptide YY induced myoelectric activity and transit of small intestinal contents in rats. Scand J Gastroenterol 20:577–582, 1985
Bueno L, Ruckebusch Y: Perinatal development of intestinal myoelectrical activity in dogs and sheep. Am J Physiol 237(1):E61-E67, 1979
Weisbrodt NW: Intestinal motility.In Physiology of the Gastrointestinal Tract. LR Johnson (ed). New York, Raven Press, 1987, pp 631–663
Aalen OO, Husebye E: Statistical analysis of repeated events forming renewal processes. Stat Med 10:1227–1240, 1991
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Husebye, E., Hellström, P.M. & Midtvedt, T. Intestinal microflora stimulates myoelectric activity of rat small intestine by promoting cyclic initiation and aboral propagation of migrating myoelectric complex. Digest Dis Sci 39, 946–956 (1994). https://doi.org/10.1007/BF02087542
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DOI: https://doi.org/10.1007/BF02087542