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An automated system for use in collecting volatile chemicals released from plants

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Abstract

A system is described for the collection of volatiles produced by plants that minimizes stress on the plant in an environment that is free from chemical impurities. Air entering a volatile collection chamber containing a plant is purified using a nonwoven fabric media infused with charcoal. A multitasking, computer-automated system is described that can simultaneously collect volatilized chemicals from plants as well as monitor and record environmental conditions associated with those collections. Collection of up to 16 samples can be made in varying sampling order, flow rates, and user-specified time periods, without disturbing the sampling environment. During the same time period, this system is capable of simultaneously monitoring up to eight environmental parameters using any type of sensor with electrical signal outputs. A multiport base assembly was designed to fit around the base of the plant permitting air samples to be collected at the bottom of the chamber. The chamber can pass ambient light so the plant may follow its natural photocycles. The entire system can be configured for continuous laboratory duty or portable field use by utilizing components that run on DC voltages. For the purpose of testing the system's performance, we determined the periodicity of the release of volatiles from red and yellow flowering four o'clock plants,Mirabilis jalaba (Nyctaginaceae). The major chemical released from four o'clocks was identified as ocimene. The onset of release occurred between 1400 and 1600 hr and increased with time with maximum amount of ocimene released during 1800–2000 hr, followed by a decrease in emission. No ocimene was detected after 2400 hr. Determination of the amount of ocimene released per flower was calculated for the 1800- to 2000-hr time period. Based on the number of open flowers during the 1800- to 2000-hr period, yellow four o'clock's released 80.9 (±7.3 SD) ng/hr/flower, while the red flowers released 51.9 (±7.0 SD) ng/hr/flower.

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References

  • Altenburger, R., andMatile, P. 1988. Circadian rhythmicity emission of fragrance in flowers ofHoya carnosa R. Br.Planta 174:248–252.

    Google Scholar 

  • Altenburger, R., andMatile, P. 1990. Further observations on rhythmic emission of fragrance in flowers.Plant 180:194–197.

    Google Scholar 

  • Biddington, N.L. 1986. The effects of mechanically-induced stress in plants—a review.Plant Growth Regul. 00:101–123.

    Google Scholar 

  • Buttery, R.G. 1981. Vegetable and Fruit Flavors, pp. 175–216,in R. Teranishi, R.A. Flath, and H. Sugisawa (eds.). Flavor Research, Recent Advances. Marcel Dekker, New York.

    Google Scholar 

  • Grob, J.J. 1982. Band broadening in space and the retention gap in capillary gas chromatography.J. Chromatogr. 237:15–23.

    Google Scholar 

  • Heath, R.R., andManukian, A. 1992. Development and evaluation of systems to collect volatile semiochemicals from insects and plants using a charcoal-infused medium for air purification.J. Chem. Ecol. 18(7):1209–1226.

    Google Scholar 

  • Heath, R.R., Landolt, P.J., Dueben, B., andLenczewski, B. 1992. Identification of floral compounds of night-blooming jessamine attractive to cabbage looper moths.Environ. Entomol. 21(4):854–859.

    Google Scholar 

  • Hyodo, H. 1991. Stress/wound ethylene, pp. 43–63,in A.K. Mattoo and J.C. Suttle (eds.). The Plant Hormone Ethylene. CRC Press, Boca Raton, Florida.

    Google Scholar 

  • Kimmerer, T.W., andKozlowski, T.T. 1982. Ethylene, ethane, acetaldehyde, and ethanol production by plants under stress.Plant Physiol. 69:840–847.

    Google Scholar 

  • Loughrin, J.H., Hamilton-Kemp, T.R., Andersen, R.A., andHildebrand, D.F. 1990a. Headspace compounds from flowers ofNicotiana tabacum and related species.J. Agric. Food Chem. 38(2):455–460.

    Google Scholar 

  • Loughrin, J.H., Hamilton-Kemp, T.R., Andersen, R.A., andHildebrand, D.F. 1990b. Volatiles from flowers ofNicotania sylvestris, N. otophora andMalus x domesetica: Headspace components and day/night changes in their relative concentrations.Phytochemistry 29(8):2473–2477.

    Google Scholar 

  • Loughrin, J.H., Hamilton-Kemp, T.R., Andersen, R.A., andHildebrand, D.F. 1991. Circadian rhythm of volatile emission from flowers ofNicotiana sylvestris andN. suaveolens.Physiol. Plant. 83:492–496.

    Google Scholar 

  • Manukian, A., andHeath, R.R. 1993. Development of an automated system to simultaneously collect volatiles and monitor environmental conditions for use in life sciences research.Sci. Comput. Autom. 9(11):27–40.

    Google Scholar 

  • Matile, P., andAltenburger, R. 1988. Rhythms of fragrance emission in flowers.Planta 174:242–247.

    Google Scholar 

  • Murphy, R.E. 1989. The fractionation gap: An optimized coupling of fused silica columns in open tubular gas chromatography. Master's thesis. University of Florida, Gainesville.

    Google Scholar 

  • Panasiuk, O. 1984. Response of Colorado potato beetles,Leptinotarsa decemlineata (Say), to volatile compounds of tansy,Tanacetum vulgare.J. Chem. Ecol. 10(9):1325–1333.

    Google Scholar 

  • Tollsten, L., andBergstrom, G. 1988. Headspace volatiles of whole plants and macerated plant parts ofBrassica andSinapis.Phytochemistry 27:4013–4018.

    Google Scholar 

  • Tumlinson, J.H., Lewis, W.J., andVet, L.E.M. 1993. How parasitic wasps find their hosts.Sci. Am. 268:100–106.

    PubMed  Google Scholar 

  • Turlings, T.C.J., andTumlinson, J.H. 1992. Systemic release of chemical signals by herbivoreinjured corn.Proc. Natl. Acad. Sci. U.S.A. 89:8399–8402.

    PubMed  Google Scholar 

  • Wyatt, R. 1983. Pollinator-plant interactions and the evolution of breeding systems, pp. 51–95,in L. Real (ed.). Pollination Biology. Academic Press, New York.

    Google Scholar 

  • Yu, Y.-B., andYang, S.A. 1980. Biosynthesis of wound ethylene.Plant Physiol. 66:281–285.

    Google Scholar 

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Heath, R.R., Manukian, A. An automated system for use in collecting volatile chemicals released from plants. J Chem Ecol 20, 593–608 (1994). https://doi.org/10.1007/BF02059600

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  • DOI: https://doi.org/10.1007/BF02059600

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