Abstract
Using a confocal microscope with a single-photon avalanche photodiode as detector, we studied photon bursts of single Rhodamine 6G (R6G) and Rhodamin B-zwitterion (RB) molecules in aqueous solution by excitation of the lowest excited singlet stateS 1 with a frequency-doubled titanium: sapphire laser. Multichannel scaler traces, the fluorescence autocorrelation function and fluorescence decay times determined by time-correlated single-photon counting have been measured simultaneously. The time-resolved fluorescence signals were analyzed with a maximum likelihood estimator. Fluorescence lifetime patterns in steps of 100 ps were generated by convolution with the excitation pulse. The lifetime of theS 1 state was derived from the Kullback-Leibler minimum discrimination information. We are able to demonstrate for the first time identification of two different single dye molecules via their characteristic fluorescence lifetimes of 1.79 ± 0.33 ns (RB) and 3.79 ± 0.38 ns (R6G) in aqueous solution.
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Dedicated to Prof. F. P. Schäfer on the occasion of his 65th birthday.
On leave from Department of Physics, Mokwon University, Taejon, 301-729, Korea
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Zander, C., Sauer, M., Drexhage, K.H. et al. Detection and characterization of single molecules in aqueous solution. Appl. Phys. B 63, 517–523 (1996). https://doi.org/10.1007/BF01828950
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DOI: https://doi.org/10.1007/BF01828950