Abstract
Hybridization analyses of cDNA clones derived from the two genomic RNAs, RNA1 and RNA2, of the walnut strain of the nepovirus cherry leafroll nepovirus (wCLRV) demonstrated a long region of high homology between the two viral RNAs. Subsequent mapping and nucleotide sequencing revealed a long, noncoding, presumably untranslated, region (3′ UTR) immediately 5′ of the terminal polyadenylate, a region that is almost identical in the two RNAs. This 3′ UTR is 1567 nucleotide residues long in RNA1. Homologies of about 80% were found with corresponding regions of genomic RNAs from other strains of CLRV, but not with the corresponding regions of other nepovirus genomic RNAs.
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The nucleotide sequence reported in this paper has been submitted to the EMBL data library and assigned the accession number Z34265.
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Borja, M., Sánchez, F., Rowhani, A. et al. Long, nearly identical untranslated sequences at the 3′ terminal regions of the genomic RNAs of cherry leafroll virus (walnut strain). Virus Genes 10, 245–252 (1995). https://doi.org/10.1007/BF01701814
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DOI: https://doi.org/10.1007/BF01701814