Abstract
Conditions for efficient protoplasting and regeneration ofAgaricus bisporus andA. bitorquis are described. Especially forA. bisporus protoplasts, high regeneration frequencies were obtained (up to 30%). The protoplasting/regeneration system can be used for routine isolation of homokaryons ofA. bisporus. Such homokaryons, derived from protoplasts containing one type of nucleus only, can easily be identified by analyzing isoenzyme banding patterns.
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Sonnenberg, A.S., Wessels, J.G. & van Griensven, L.J. An efficient protoplasting/regeneration system forAgaricus bisporus andAgaricus bitorquis . Current Microbiology 17, 285–291 (1988). https://doi.org/10.1007/BF01571330
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DOI: https://doi.org/10.1007/BF01571330