Abstract
In an iron-depleted broth culture of cowpeaRhizobium (a peanut isolate), phenolate type of compounds were detected. Chemical characterization showed the presence of 2,3-dihydroxy benzoic acid (DHBA) and 3,4-DHBA in the siderophore extract. Lysine and alanine were identified as conjugated amino acids of the siderophore. Maximum concentration of the siderophore in the culture supernatant was found after 24 h of growth. The compounds in the extracted siderophore induced growth ofRhizobium in a medium containing EDTA. Addition of lysine and alanine in the growth medium (20 mM each) led to a fourfold increase in siderophore production.
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Jadhav, R.S., Desai, A.J. Isolation and characterization of siderophore from cowpeaRhizobium (peanut isolate). Current Microbiology 24, 137–141 (1992). https://doi.org/10.1007/BF01568978
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DOI: https://doi.org/10.1007/BF01568978