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Bioassay of interleukins

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Journal of tissue culture methods

Summary

Sensitive microassay methodologies are described for the bioassay of interleukins 1, 2, 3, and 4 in both serum-free and serum-containing culture supernatants. Interleukins 2, 3, and 4 are measured directly by their growth-promoting activities on the CT6, FDC-P1, and HT-2 indicator cell lines, respectively. Interleukin 1 is assayed indirectly by its ability to stimulate interleukin 2 production by LBRM-33 1A5 lymphoma cells in the presence of phytohemagglutinin. Quantitation is based on measurement of [3H]thymidine incorporation into cellular DNA. The bioassays are performed in microcultures and are semiautomated so that panel testing of small volumes of interleukin-containing culture supernatants can be readily accomplished within 3 d.

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Authors and Affiliations

  1. Department of Immunology, Mayo Clinic and Foundation, 55905, Rochester, Minnesota

    Robert T. Abraham, Steffan N. Ho & David J. McKean

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  1. Robert T. Abraham
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  2. Steffan N. Ho
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  3. David J. McKean
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Abraham, R.T., Ho, S.N. & McKean, D.J. Bioassay of interleukins. Journal of Tissue Culture Methods 10, 93–99 (1986). https://doi.org/10.1007/BF01404599

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