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Restriction endonuclease mapping of the genome of feline herpesvirus type 1

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Summary

Restriction endonuclease mapping was used to determine the molecular structure of feline herpesvirus type 1 (FHV-1) strain B 927. FHV-1 DNA purified from infected cells by pulsed field gel electrophoresis was partially restricted with Sau 3A to generate a series of overlapping fragments of 10–20 kb. EMBL 4 DNA was digested with Bam HI and Sal I and viral DNA was inserted into the “stuffer” region. Recombinant phage were selected onE. coli strain Q 359. Single and double digestions with the enzymes Eco RI, Bam HI, Sal I, Sst I and Xho I enabled construction of physical maps of the genome. The FHV-1 genome is approximately 126 kbp in length and is composed of a long unique region of 99 kbp and a short region of 27 kbp comprising an unique sequence of 8–9 kbp flanked by inverted repeats of 7–8.5 kbp.

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Authors and Affiliations

  1. Department of Veterinary Medicine, Langford House, Langford, Bristol, England

    A. Grail & D. A. Harbour

  2. Institute of Molecular and Cell Biology, National University of Singapore, Singapore

    W. Chia

Authors
  1. A. Grail
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  2. D. A. Harbour
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  3. W. Chia
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Grail, A., Harbour, D.A. & Chia, W. Restriction endonuclease mapping of the genome of feline herpesvirus type 1. Archives of Virology 116, 209–220 (1991). https://doi.org/10.1007/BF01319243

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  • DOI: https://doi.org/10.1007/BF01319243

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