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Salmonid viruses: Double infection of RTG-2 cells with Egtved and infectious pancreatic necrosis viruses

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Summary

Egtved and infectious pancreatic necrosis (IPN) viruses were applied at high multiplicity alone and in combination to RTG-2 cells in liquid medium. Procedures were used which selectively suppressed one or the other virus, and the yields were determined after 64 hours at 15°C. The viruses showed an increase of 280 to 450 times the immediate post-adsorption level. Plaques of IPN and Egtved viruses showed some differences which can be helpful in distinguishing between the two agents. Detailed methods and results are given.

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  1. Ken Wolf

    Present address: Eastern Fish Disease Laboratory, U.S.D.I. Bureau of Sport Fisheries and Wildlife, Kearneysville, West Virginia, USA

Authors and Affiliations

  1. State Veterinary Serum Laboratory, Århus, Denmark

    P. E. Vestergård Jørgensen

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  1. Ken Wolf
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  2. P. E. Vestergård Jørgensen
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Wolf, K., Jørgensen, P.E.V. Salmonid viruses: Double infection of RTG-2 cells with Egtved and infectious pancreatic necrosis viruses. Archiv f Virusforschung 29, 337–342 (1970). https://doi.org/10.1007/BF01249888

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  • DOI: https://doi.org/10.1007/BF01249888

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