Abstract
There are at least three transcriptionally active human ADP/ATP translocase genes. We have isolated seven ADP/ATP translocase pseudogenes from recombinant human genomic libraries. Each pseudogene sequence had more than 85% identity with the sequence of the human ADP/ATP translocase cDNA derived from fibroblast mRNA, but each had mutations that precluded synthesis of a functional protein. Using an intro probe derived from a partial clone of the human fibroblast ADP/ATP translocase gene, we localized the gene to chromosome Xq13 → Xq25-26. The gene encoding the skeletal muscle translocase has previously been shown to be on chromosome 4. Therefore, the human ADP/ATP translocase genes are members of a multigene family that includes pseudogenes and has been dispersed to at least two chromosomes.
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Schultheiss, H.P., Berg, P., and Klingenberg, M. (1984).Clin. Exp. Immunol. 58596–602.
Cozens, A.L., Runswick, M.J., and Walker, J.E. (1989).J. Mol. Biol. 206261–280.
Battini, R., Ferrari, S., Kaczmarek, L., Calabretta, B., Chen, S.-T., and Baserga, R. (1987).J. Biol. Chem. 2624355–4359.
Li, K., Warner, C.K., Hodge, J.A., Minoshima, S., Kudoh, J., Fukuyama, R., Maekawa, M., Shimizu, Y., Shimizu, N., and Wallace, D.C. (1989).J. Biol. Chem. 26413998–14004.
Neckelman, N., Li, K., Wade, R.P., Shuster, R., and Wallace, D.C. (1987).Proc. Natl. Acad. Sci. U.S.A. 847580–7584.
Houldsworth, J., and Attardi, G. (1988).Proc. Natl. Acad. Sci. U.S.A. 85377–381.
Southern, E.M. (1975).J. Mol. Biol. 98503–517.
Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982).Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).
Feinberg, A.P., and Vogelstein B. (1983).Anal. Biochem. 1326–13.
Maxam, A.M., and Gilbert, W. (1980).Methods Enzymol. 65499–560.
Huebner, K., Isobe, M., Gasson, J.C., Golde, D.W., and Croce, C.M. (1986).Am. J. Hum. Genet. 38819–826.
Huebner, K., ar-Rushdi, A., Griffin, C.A., Isobe, M., Kozak, C., Emanuel, B.S., Nagarajan, L., Cleveland, L.L., Bonner, T.T., Goldsborough, M.D., Croce, C.M., and Rapp, U. (1986).Proc. Natl. Acad. Sci. U.S.A. 833934–3938.
Durst, M., Croce, C.M., Gissmann, L., Schwarz, E., and Huebner, K. (1987).Proc. Natl. Acad. Sci. U.S.A. 841070–1074.
Russo, G., Isobe, M., Pegorano, L., Finian, J., Nowell, P.C., and Croce, C.M. (1988).Cell 53137–144.
Bauer, S.R., Huebner, K., Budarf, M., Finan, J., Erikson, J., Emanual, B.S., Nowell, P.C., Croce, C.M., and Melchers, F. (1988).Immunogenetics 28328–333.
Scott, B.F., Phillips, J.A., and Migeon, B.R. (1979).Proc. Natl. Acad. Sci. U.S.A. 764563–4565.
Schultheiss, H.-P., and Klingenberg, M. (1984).Eur. J. Biochem. 143599–605.
Schultheiss, H.-P., and Klingenberg, M. (1985).Arch. Biochem. Biophys. 239273–279.
Schultheiss, H.P., and Bolte, H.D. (1985).J. Mol. Cell Cardiol. 17603–617.
Gay, N.J., and Walker, J.E. (1985).EMBO J. 43519–3524.
Hirschhorn, R.R., Aller, P., Yuan, Z.-A., Gibson, C.W., and Baserga, R. (1984).Proc. Natl. Acad. Sci. U.S.A. 816004–6008.
Kaczmarek, L., Calabretta, B., and Baserga, R. (1985).Proc. Natl. Acad. Sci. U.S.A. 825375–5379.
Kaczmarek, L., Calabretta, B., and Baserga, R. (1985).Biochem. Biophys. Res. Commun. 133410–416.
Reilly, C.F., Kindy, M.S., Brown, K.E., Rosenberg, R.D., and Sonnenshein, G.E. (1989).J. Biol. Chem. 2646990–6995.
Buckler, A.J., Rothstein, T.L., and Sonenshein, G.E. (1988).Mol. Cell. Biol. 81371–1375.
Barth, P.G., Scholte, H.R., Berden, J.A., Van Der Klei-Van Moorsel, J.M., Luyt-Houwen, I.E.M., Van'T Veer-Korthof, E.Th., Van Der Harten, J.J., and Sobotka-Plojhar, M.A. (1983).J. Neurol. Sci. 62327–355.
Neustein, H.B., Lurie, P.R., Dahms, B., and Takahashi, M. (1979).Pediatrics 6424–29.
Stallings, R.L., Olson, E., Strauss, A.W., Thompson, L.H., Bachinski, L.L., and Siciliano, M.J. (1988).Am. J. Hum. Genet. 43144–151.
Runswick, M.J., Powell, S.J., Nyren, P., and Walker, J.E. (1987).EMBO J. 61367–1373.
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Chen, ST., Chang, CD., Huebner, K. et al. A human ADP/ATP translocase gene has seven pseudogenes and localizes to chromosome X. Somat Cell Mol Genet 16, 143–149 (1990). https://doi.org/10.1007/BF01233044
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DOI: https://doi.org/10.1007/BF01233044