Abstract
An improved method is introduced for the determination of the quantum yield of photosystem I. The new method employs saturating light pulses with steep rise characteristics to distinguish, in a given physiological state, centers with an open acceptor side from centers with a reduced acceptor side. The latter do not contribute to PSI quantum yield (ΦI). Oxidation of P700 is measured by a rapid modulation technique using the absorbance change around 830 nm. The quantum yield ΦI is calculated from the amplitude of the rapid phase of absorbance change (ΔA; 830 nm) upon application of a saturation pulse in a given state, divided by the maximal ΔA (830 nm) which is induced by a saturation pulse with far-red background illumination. Using this technique, ΦI can be determined even under conditions of acceptor-side limitation, as for example in the course of a dark-light induction period or after elimination of CO2 from the gas stream. Thus determined ΦI values display a close-to-linear relationship with those for the quantum yield of PSII (ΦII) calculated from chlorophyll fluorescence parameters. It is concluded that the proposed method may provide new information on the activity of the PSI acceptor side and thus help to separate the effects of acceptorside limitation from those of cyclic PSI, whenever a non-linear relationship between ΦII and the P700-reduction level is observed.
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Abbreviations
- ΔA:
-
absorbance change
- ΦI :
-
quantum yield of photosystem I
- ΦII :
-
quantum yield of photosystem II
- PAR:
-
photosynthetically active radiation
References
Asada, K., Heber, U., Schreiber, U. (1993) Electron flow to intersystem chain from stromal components and cyclic electron flow in maize chloroplasts as detected in intact leaves by monitoring P700 and chlorophyll fluorescence. Plant Cell Physiol.34, 39–50
Foyer, C., Furbank, R., Harbinson, J., Horton, P. (1990) The mechanisms contributing to photosynthetic control of electron transport by carbon assimilation in leaves. Photosynth. Res.25, 83–100
Foyer, C.H., Lelandais, M., Harbinson, J. (1992) Control of the quantum efficiencies of photosystem I and II, electron flow, and enzyme activation following dark-to-light transitions in pea leaves. Plant Physiol.99, 979–986
Genty, B., Briantais, J.-M., Baker, N.R. (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta990, 87–92
Genty, B., Wonders, J. Baker N.R. (1990) Non-photochemical quenching of Fo in leaves is emission wavelength dependent: consequences for quenching analysis and its interpretation. Photosynth. Res.26, 133–139
Harbinson, J., Foyer, C. (1991) Relationships between the efficiencies of photosystems I and II and stromal redox state in CO2-free air. Evidence for cyclic electron flow in vivo. Plant Physiol.97, 41–49
Harbinson, J., Hedley, C.L. (1989) The kinetics of P700 reduction in leaves: a novel in situ probe of thylakoid functioning. Plant Cell Environ.12, 357–369
Harbinson, J., Woodward, F.I. (1987) The use of light-induced absorbance changes at 830 nm to monitor the oxidation state of P700 in leaves. Plant Cell Environ.10, 131–140
Harbinson, J., Genty, B., Baker, N.R. (1989) Relationship between the quantum efficiencies of photosystems I and II in pea leaves. Plant Physiol.90, 1029–1034
Harbinson, J., Genty, B., Foyer, C.H. (1990) Relationship between photosynthetic electron transport and stromal enzyme activitiy in pea leaves. Toward an understanding of the nature of photosynthetic control. Plant Physiol.94, 545–553
Heber, U., Walker, D. (1992) Concerning a dual function of coupled cyclic electron transport in leaves. Plant Physiol.100, 1621–1626
Heber, U., Schreiber, U., Siebke, K., Dietz, K.-J. (1990) Relationship between light-driven electron transport, carbon reduction and carbon oxidation in photosynthesis. In: Perspectives in biochemical and genetic regulation of photosynthesis, pp. 17–37, Zelitch, J., ed. Wiley-Liss, New York
Herbert, S.K., Fork, D.C., Malkin, S.H. (1990) Photoacoustic measurements in vivo of energy storage by cyclic electron flow in algae and higher plants. Plant Physiol.94, 926–934
Horton, P. (1989) Interactions between electron transport and carbon assimilation: regulation of light harvesting and photochemistry. In: Photosynthesis, pp. 393–406, Briggs, W.R., ed. Alan R. Liss Inc., New York
Klughammer, C., Schreiber, U. (1991) Analysis of light-induced absorbance changes in the near-infrared spectral region. I. Characterization of various components in isolated chloroplasts. Z. Naturforsch.46c, 233–244
Laisk, A., Oja, V., Heber, U. (1993) Steady-state and induction kinetics of photosynthetic electron transport related to donor side oxidation and acceptor side reduction of photosystem I in sunflower leaves. Photosynthetica, in press
Lechtenberg, D., Voss, B., Weis, E. (1989) Regulation of photosynthesis: Photosynthetic control and thioredoxin-dependent enzyme regulation. In: Current research in photosynthesis, vol. IV, pp. 171–174, Baltscheffsky, M., ed. Kluwer Academic Publishers, Dordrecht, The Netherlands
Mi, H., Endo, T., Schreiber, U., Ogawa, T., Asada, K. (1992) Electron donation from cyclic and respiratory flows to the photosynthetic intersystem chain is mediated by pyridine nucleotide dehydrogenase in the cyanobacteriumSynechocystis PCC 6803. Plant Cell Physiol.33, 1233–1237
Neubauer, C., Schreiber, U. (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: I. Saturation characteristics and partial control by the photosystem II acceptor side. Z. Naturforsch.42c, 1246–1254
Peterson, R.B. (1991) Effects of O2 and CO2 concentrations on quantum yields of photosystem I and II in tobacco leaf tissue. Plant Physiol.97, 1388–1394
Scheibe, R., Stitt, M. (1988) Comparison of NADP-malate dehydrogenase activation, QA reduction and O2 evolution in spinach leaves. Plant Physiol. Biochem.26, 473–482
Schreiber, U. (1986) Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer. Photosynth. Res.9, 261–272
Schreiber, U., Schliwa, U., Bilger, W. (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulated fluorometer. Photosynth. Res.10, 51–62
Schreiber, U., Klughammer C., Neubauer, C. (1988) Measuring P700 absorbance changes around 830 nm with a new type of pulse modulation system. Z. Naturforsch.43c, 686–698
Van Kooten, O., Snel, J.F.H. (1990) The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynth. Res.25, 147–150
Walker, D.A. (1992) Excited leaves. New Phytol121, 325–345
Weis, E., Berry, J. (1987) Quantum efficiency of photosystem II in relation to energy-dependent quenching of chlorophyll fluorescence. Biochim. Biophys. Acta894, 198–208
Weis, E., Ball, J.R., Berry, J. (1987) Photosynthetic control of electron transport in leaves ofPhaseolus vulgaris. Evidence for regulation of photosystem 2 by the proton gradient. In: Progress in photosynthesis research, vol. 2, pp. 553–556, Biggins, J., ed. Martinus-Nijhoff Publishers, Dordrecht
Weis, E., Lechtenberg, D. (1989) Fluorescence analysis during steady-state photosynthesis. Philos. Trans. R. Soc. Lond. B Biol. Sci.323, 253–268
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This work was supported by the Deutsche Forschungsgemeinschaft (SFB 176 “Molekulare Grundlagen der Signalübertragung und des Stofftransportes in Membranen” and SFB 251 “Ökologie, Physiologie und Biochemie pflanzlicher Leistung unter Streß”).
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Klughammer, C., Schreiber, U. An improved method, using saturating light pulses, for the determination of photosystem I quantum yield via P700+-absorbance changes at 830 nm. Planta 192, 261–268 (1994). https://doi.org/10.1007/BF01089043
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DOI: https://doi.org/10.1007/BF01089043