Skip to main content
SpringerLink
Log in

Carbohydrate binding specificities of several poly-N-acetyllactosamine-binding lectins

  • Published:
Glycoconjugate Journal Aims and scope Submit manuscript

Abstract

The structural requirements for the interaction of the Asn-linked poly-N-acetyllactosamine-type oligosaccharide moieties of glycoproteins with variousN-acetylglucosamine-binding lectins were investigated by means of affinity chromatography on immobilized lectin-Sepharose columns.

High molecular weight glycopeptides containing poly-N-acetyllactosamine-type oligosaccharides obtained by Pronase digestion of human erythrocyte ghosts were treated with 0.1 M trifluoroacetic acid at 100°C for 40 min and then several oligosaccharide fragments were purified with an amino-bonded silica column. Among these oligosaccharide fragments, trisaccharide Galβ1-4GlcNAcβ1-6Galol bound to the wheat germ agglutinin (WGA)- and pokeweed mitogen (PWM)-Sepharose columns, and also showed affinity to theDatura stramonium agglutinin (DSA)-,Lycopersicon esculentum (tomato) agglutinin-andSolanum tuberosum (potato) agglutinin-Sepharose columns. Pentasaccharide Galβ1-4GlcNAcβ1-3(Galβ1-4GlcNAcβ1-6)Galol showed weaker affinity to the WGA- and PWM-Sepharose columns, compared to the trisaccharide. Trisaccharide GlcNAcβ1-3(GlcNAcβ1-6)Galol showed weak affinity to the WGA-Sepharose column and did not show any affinity to the other lectin-Sepharose columns. Hexasaccharide Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcol bound only to the DSA-Sepharose column, indicating that only DSA does not require a GlcNAcβ(1-6)-linkage for interaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

HPLC:

high performance liquid chromatography

WGA:

wheat germ agglutinin

PWM:

pokeweed mitogen

DSA:

Datura stramonium agglutinin

LEA:

Lycopersicon esculentum (tomato) agglutinin

STA:

Solanum tuberosum (potato) agglutinin

EVA:

Erythrina variegata agglutinin

PBS:

10 mM sodium phosphate buffer, pH 7.2, containing 0.15 M NaCl

Galol:

galactitol

GlcNAcol:

N-acetylglucosaminitol

References

  1. Goldstein IJ, Hughes RC, Monsigny M, Osawa T, Sharon N (1980) Nature 285: 66.

    Google Scholar 

  2. Cummings RD, Kornfeld S (1982) J Biol Chem 257:11235–40.

    Google Scholar 

  3. Yamamoto K, Tsuji T, Tarutani O, Osawa T (1985) Biochim Biophys Acta 838:84–91.

    Google Scholar 

  4. Reitman ML, Trowbridge IS, Kornfeld, S (1982) J Biol Chem 257:10357–63.

    Google Scholar 

  5. Stanley P, Vivona G, Atkinson PH (1984) Arch Biochem Biophys 230:363–74.

    Google Scholar 

  6. Cummings RD, Kornfeld S (1984) J Biol Chem 259:6253–60.

    Google Scholar 

  7. Debray H, Montreuil J, Lis H, Sharon N (1986) Carbohydr Res 151:359–70.

    Google Scholar 

  8. Irimura T, Nicolson GL (1983) Carbohydr Res 115:209–20.

    Google Scholar 

  9. Nicolson GL, Irimura T (1984) Biol Cell 51:157–64.

    Google Scholar 

  10. Yamashita Y, Imai Y, Osawa T (1989) Mol Immunol 26:905–13.

    Google Scholar 

  11. Sharon N (1983) Adv Immunol 34:213–98.

    Google Scholar 

  12. Okada T, Ezawa K, Imai Y, Osawa T (1986) Cancer Res 46:5611–17.

    Google Scholar 

  13. Muramatsu T (1988) J Cell Biol 36:1–14.

    Google Scholar 

  14. Tsuji T, Irimura T, Osawa T (1981) J Biol Chem 256:10497–502.

    Google Scholar 

  15. Fukuda M, Dell A, Oates JE, Fukuda MN (1984) J Biol Chem 259:8260–73.

    Google Scholar 

  16. Fukuda M, Spooncer E, Oates JE, Dell A, Klock JC (1984) J Biol Chem 259: 10925–35.

    Google Scholar 

  17. Spooncer E, Fukuda M, Klock JC, Oates JE, Dell A (1984) J Biol Chem 259: 4792–801.

    Google Scholar 

  18. Muramatsu H, Muramatsu T, Avner P (1984) Cancer Res 42:1744–52.

    Google Scholar 

  19. Merkle RK, Cummings RD (1987) J Biol Chem 282:8179–89.

    Google Scholar 

  20. Pierce M, Arango J (1986) J Biol Chem 261:10772–77.

    Google Scholar 

  21. Fukuda M (1985) Biochim Biophys Acta 780:119–50.

    Google Scholar 

  22. Watanabe K, Hakomori S, Childs RA, Feizi T (1979) J Biol Chem 254:3221–28.

    Google Scholar 

  23. Fukuda M, Fukuda MN, Papayannopoulou T, Hakomori S (1980) Proc Natl Acad Sci USA 74:3474–78.

    Google Scholar 

  24. Feizi T (1985) Nature 314:53–57.

    Google Scholar 

  25. Yamamoto K, Tsuji T, Matsumoto I, Osawa T (1981) Biochemistry 20:5894–99.

    Google Scholar 

  26. Gallagher JT, Morris A, Dexter T (1985) Biochem J 231:115–22.

    Google Scholar 

  27. Renkonen O, Makinen P, Hard K, Helin J, Penttila L (1988) Can J Biochem Cell Biol 66:449–53.

    Google Scholar 

  28. Irimura T, Nicolson GL (1983) Carbohydr Res 120:187–95.

    Google Scholar 

  29. Katagiri Y, Yamamoto K, Tsuji T, Osawa T (1983) Carbohydr Res 120:283–92.

    Google Scholar 

  30. Cummings RD, Kornfeld S (1984) J Biol Chem 259:6253–60.

    Google Scholar 

  31. Yamashita K, Totani K, Ohkura T, Takasaki S, Goldstein IJ, Kobata A (1987) J Biol Chem 262:1602–7.

    Google Scholar 

  32. Allen AK, Neuberger A (1973) Biochem J 135:307–14.

    Google Scholar 

  33. Cuatrecasas P, Anfinsen CB (1971) Methods Enzymol 22:345–78.

    Google Scholar 

  34. Ciucanu I, Kerek F (1984) Carbohydr Res 131:209–17.

    Google Scholar 

  35. Yamashita K, Tachibana Y, Kobata A (1976) Arch Biochem Biophys 174:582–91.

    Google Scholar 

  36. Stellner K, Saito H, Hakomori S (1973) Arch Biochem Biophys 155:464–72.

    Google Scholar 

  37. Li H, Kawashima H, Yamamoto K, Osawa T (1990) Glycoconjugate J 7:311–22.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, 113, Tokyo, Japan

    Hiroto Kawashima, Shinobu Sueyoshi, Hua Li, Kazuo Yamamoto & Toshiaki Osawa

Authors
  1. Hiroto Kawashima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shinobu Sueyoshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hua Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kazuo Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Toshiaki Osawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kawashima, H., Sueyoshi, S., Li, H. et al. Carbohydrate binding specificities of several poly-N-acetyllactosamine-binding lectins. Glycoconjugate J 7, 323–334 (1990). https://doi.org/10.1007/BF01073376

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01073376

Keywords

Search

Navigation