Summary
Unusually low culture temperature, such as 20°C, was shown to be preferable for the synthesis of active human interferon-β (IFN-β) inE. coli harboring a recombinant plasmid. TheE. coli cells cultured at 20°C gave 8.6-fold higher IFN-β activity than those cultured at 37°C. However, almost the equal amounts of IFN-β protein were accumulated in both cells cultured at 20°C and at temperature higher than 20°C, suggesting that IFN-β might exist as an active form in the cells cultured at 20°C, while as a rather denatured form in the cells cultured at higher temperature.
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Mizukami, T., Komatsu, Y., Hosoi, N. et al. Production of active human interferon-β inE. coli I. Preferential production by lower culture temperature. Biotechnol Lett 8, 605–610 (1986). https://doi.org/10.1007/BF01025964
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DOI: https://doi.org/10.1007/BF01025964