Abstract
Free amino acids and cholinergic enzymes were investigated in the cerebellum of reeler and weaver mice in an attempt to identify the neurotransmitter characteristic of the granule cell population and to clarify any neurotransmitter abnormalities of their pre- and postsynaptic neurons induced by their depletion. The data indicate that glutamic acid may be the neurotransmitter of the granule cells. Pre- and postsynaptic neurotransmitter activity seemed not to be markedly altered in cerebellar granule cell dysgenesis.
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Eccles, J.C., Ito, M., andSzentagothai, J. 1967. The Cerebellum as a Neuronal Machine, Springer-Verlag, New York.
Margolis, G., andKilham, L. 1968. Virus-induced cerebellar hypoplasia. Pages 113–146,in Zimmerman, H.M. (ed.), Infections of the nervous system. Assoc. Research in Nervous and Mental Disease, Vol. XLIV, The Williams and Wilkins Company, Baltimore.
Sidman, R.L. 1968. Development of interneuronal connections in brains of mutant mice. Pages 163–193,in Carlson, F.D. (ed.), Physiological and Biochemical Aspects of Nervous Integration. Prentice-Hall, Inc., Englewood Cliffs, New Jersey.
Shrier, B.K., andThompson, E.J. 1974. On the role of glial cells in mammalian nervous system. J. Biol. Chem. 249(6):1769–1780.
Siggins, G.R., Hoffer, B.J., Oliver, A.P., andBloom, F.E. 1971. Activation of a central noradrenergic projection to cerebellum. Nature 233:481–483.
Tebécis, A.K. 1974. Cerebellum. Pages 86–115,in Transmitters and Identified Neurons in the Mammalian Central Nervous System, Scientechnica (Publishers) Ltd., Bristol.
Altman, J., Anderson, W.T., andWright, K.A. 1967. Selective destruction of microneurons of the cerebellar cortex with fractionated low-dose X-rays. Exp. Neurol. 17:481–497.
Hamburgh, M. 1963. Analysis of the postnatal developmental effects of “Reeler”, a neurological mutation in mice. A study in developmental genetics. Dev. Biol. 8:165–185.
Llinas, R., Hillman, D.E., andPrecht, W. 1973. Neuronal circuit reorganization in mammalian agranular cerebellar cortex. J. Neurobiol. 4:69–94.
Altman, J., andAnderson, W.J. 1972. Experimental reorganization of the cerebellar cortex: I. Morphological effects of elimination of all microneurons with prolonged X-irradiation started at birth. J. Comp. Neurol. 146:355–405.
Altman, J., andAnderson, W.J. 1973. Experimental reorganization of the cerebellar cortex: II. Effects of elimination of most microneurons with prolonged X-irradiation started at four days. J. Comp. Neurol. 149:123–152.
Rakic, P., andSidman, R.L. 1973. Organization of cerebellar cortex secondary to deficit of granule cells in weaver mutant mice. J. Comp. Neurol. 152:133–162.
Rakic, P., andSidman, R.L. 1973. Sequence of developmental abnormalities leading to granule cell deficit in cerebellar cortex of weaver mutant mice. J. Comp. Neurol. 152:103–132.
Ellman, G.L., Courtney, K.D., Andres, V., andFeatherstone, R.M. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7:88–95.
Fonnum, F. 1966. A radiochemical method for the estimation of choline acetyltransferase. Biochem. J. 100:479–484.
Hudson, D.B., Vernadakis, A., andTimiras, P.S. 1970. Regional changes in amino acid concentration in the developing brain and the effects of neonatal administration of estradiol. Brain Res. 23:213–222.
Bloom, F.E. 1972. Amino acids and polypeptides in neuronal function. Neurosci. Res. Program Bull. 10:127–220.
Curtis, D.R., andJohnston, G.A.R. 1970. Amino acid transmitters. Pages 115–131,in Lajtha, A. (ed.), Handbook of Neurochemistry, Plenum Press, New York.
Davison, A.N., andKaczmarek, L.K. 1971. Taurine—a possible neurotransmitter. Nature (London) 234:107–115.
Johnson, J.L. 1972. Glutamic acid as synaptic transmitter in the nervous system. A review. Brain Res. 37:1–19.
Hudson, D.B., Valcana, T., andTimiras, P.S. 1976. Monoamine metabolism in the developing rat brain and effects of ionizing radiation. Brain Res. (in press).
Landis, S.C., Shoemaker, W.J., Schlumpf, M., andBloom, F.E. 1975. Catecholamines in mutant mouse cerebellum: Fluorescence microscopic and chemical studies. Brain Res. 93:253–266.
Valcana, T., Hudson, D., andTimiras, P.S. 1972. Effects of X-irradiation on the content of amino acids in the developing rat cerebellum. J. Neurochem. 19:2229–2232.
Valcana, T., Liao, C., andTimiras, P.S. 1974. Effects of X-radiation on the development of the cholinergic system of the rat brain. II. Investigation of alterations in acetylcholine content. Environ. Physiol. Biochem. 4:58–63.
Valcana, T., Liao, C., andTimiras, P.S. 1974. Effects of X-radiation on the subcellular distribution of cholinergic enzymes in the developing rat cerebellum. Brain Res. 73:105–120.
Valcana, T., andTimiras, P.S. 1974. Effects of X-radiation on the development of the cholinergic system of the rat brain. I. Study of alterations in choline acetyltransferase and acetylcholinesterase activity and acetylcholinesterase synthesis. Environ. Physiol. Biochem. 4:47–57.
Valcana, T., Vernadakis, A., andTimiras, P.S. 1969. Effects of neonatal X-radiation on choline acetyltransferase activity in various areas of the developing central nervous system. Pages 887–897,in Sikov, M.R., andMahlum, D.D. (eds.), Radiation Biology of the Fetal and Juvenile Mammal, U.S.A.E.C. Division of Technical Information Extension, Oak Ridge, Tennessee.
Young, A., Oster-Granite, M., Herndon, R., andSnyder, S. 1974. Glutamic acid: Selective depletion by viral induced granule cell loss in hamster cerebellum. Brain Res. 73:1–13.
Silver, A. 1967. Cholinesterases of the central nervous system with special reference to the cerebellum. Int. Rev. Neurobiol. 10:57–109.
Woodward, D.J., Hoffer, B.J., Siggins, G.R., andBloom, F.E. 1971. The ontogenetic development of synaptic junctions, synaptic activation and responsiveness to neurotransmitter substances in rat cerebellar Purkinje cells. Brain Res. 34:73–97.
Bignami, A., andDuhl, D. 1974. The development of Bergman glia in mutant mice with cerebellar malformations: Reeler, staggerer and weaver. Immunofluorescence study with antibodies to the glial fibrillary acidic protein. J. Comp. Neurol. 155:219–230.
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Hudson, D.B., Valcana, T., Bean, G. et al. Glutamic acid: A strong candidate as the neurotransmitter of the cerebellar granule cell. Neurochem Res 1, 73–81 (1976). https://doi.org/10.1007/BF00965633
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DOI: https://doi.org/10.1007/BF00965633