Summary
The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5–8 times higher than human cells. Ionomycinelicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion.
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References
Bosia A, Spangenberg P, Ghigo D, Heller R, Lösche W, Pescarmona GP, Till U (1985) Effect of GSH depletion by 1-chloro-2,4-dinitrobenzene on human platelet aggregation, arachidonic acid oxidative metabolism and cytoskeletal proteins. Thromb Res 37: 423–434
Bredt DS, Snyder SH (1989) Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum. Proc Natl Acad Sci USA 86: 9030–9033
Ghigo D, Alessio P, Foco A, Bussolino F, Costamagna C, Heller R, Garbarino G, Pescarmona GP, Bosia A (1993) Nitric oxide synthesis is impaired in glutathionedepleted human umbilical vein endothelial cells. Am J Physiol 265: C728-C732
Ghigo D, Arese M, Todde R, Vecchi A, Silvagno F, Costamagna C, Dong QG, Alessio M, Heller R, Soldi R, Trucco F, Garbarino G, Pescarmona GP, Mantovani A, Bussolino F, Bosia A (1995) Middle T antigen-transformed endothelial cells exhibit an increased activity of nitric oxide synthase. J Exp Med 181: 9–19
Griscavage JM, Fukuto JM, Komori Y, Ignarro LJ (1994) Nitric oxide inhibits neuronal nitric oxide synthase by interacting with the heme prosthetic group. J Biol Chem 269: 21644–21649
Mita I (1981) Chemistry, pharmacology and current clinical applications of tiopronin. In: Williams R, Gasbarrini G, Davis M (eds) Recent advances in 2-MPG treatment of liver diseases. Editrice Compositori, Bologna, pp 3–10
Nathan C, Xie Q-w (1994) Nitric oxide synthases: roles, tolls, and controls. Cell 78: 915–918
Schmidt HHHW, Walter U (1994) NO at work. Cell 78: 919–925
Stamler JS, Singel DJ, Loscalzo J (1992) Biochemistry of nitric oxide and its redoxactivated forms. Science 258: 1898–1902
Williams RL, Courtneidge SA, Wagner EF (1988) Embryonic lethalities and endothelial tumors in chimeric mice expressing polyoma virus middle T oncogene. Cell 52: 121–131
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Ghigo, D., Geromin, D., Franchino, C. et al. Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial cells. Amino Acids 10, 277–281 (1996). https://doi.org/10.1007/BF00807330
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DOI: https://doi.org/10.1007/BF00807330