Summary
Fibroblast cultures were started from synovial tissue of normal controls and patients with seronegative and seropositive rheumatoid arthritis, juvenile rheumatoid arthritis and chronic non-rheumatoid synovitis. Glycosaminoglycan synthesis of the cultures was studied by metabolic labeling with 3H-glucosamine. Molecular weight of hyaluronic acid produced and secreted into culture medium by these fibroblast strains was studied by gel filtration in a Sepharose 2 B column. All fibroblast strains from joints with active inflammation produced hyaluronate with decreased molecular weight. The synthesis of underpolymerized hyaluronate thus appears to be a property of activated synovial fibroblasts common to rheumatoid and non-rheumatoid inflammation.
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References
Seppälä P (1964) Synovial fluid in rheumatoid arthritis. Scand J Clin Lab Invest 16 (Suppl 79):1–107
Castor CW, Dorstewitz EL (1966) Abnormalities of connective tissue cells cultured from patients with rheumatoid arthritis. I. Relative unresponsiveness of rheumatoid synovial cells to hydrocortisone. J Lab Clin Med 68:300–313
Vuorio E (1977) Rheumatoid disease in cultured human synovial cells. Scand J Clin Lab Invest 37 (Suppl 149):1–72
Balazs EA, Gibbs DA (1970) The rheological properties and biological function of hyaluronic acid. In: Balazs EA (ed) Chemistry and molecular biology of the intercellular matrix, vol 3. Academic Press, New York, pp 1241–1253
McCarty DJ (1979) Synovial fluid. In: McCarty DJ (ed) Arthritis and allied conditions, 9 edn. Lea and Febiger, Philadelphia, pp 51–69
Blåfield L (1982) Pro gradu research paper, Departments of Biochemistry and Pharmacy, Åbo Akademi, Turku, Finland
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Saarni H, Tammi M (1977) A rapid method for separation and assay of radiolabeled mucopolysaccharides from cell culture medium. Anal Biochem 81:40–46
Kim JJ, Conrad HE (1976) Kinetics of mucopolysaccharide and glycoprotein synthesis by chick embryo chondrocytes. J Biol Chem 251:6210–6217
Hronowski L, Anastassiades TP (1980) The effect of cell density on net rates of glycosaminoglycan synthesis and secretion by cultured rat fibroblasts. J Biol Chem 255: 10091–10099
Stephens RW, Gosh P, Taylor TRF (1975) The characterization and function of the polysaccharidases of human synovial fluid in rheumatoid and osteoarthritis. Biochim Biophys Acta 399:101–112
Castor CW (1972) Connective tissue activation III. Observations on the mechanism of action of connective tissue activating peptide. J Lab Clin Med 79:285–301
Jalkanen M (1981) Connective tissue activating macromolecules in macrophage culture medium. Connect Tissue Res 9:19–24
Comper WD, Laurent TC (1978) Physiological functions of connective tissue polysaccharides. Physiol Rev 58:255–315
Vuorio E, Takala, I, Pulkki K, Einola S (1979) Effect of sodium aurothiomalate on hyaluronic acid synthesis in normal and rheumatoid synovial fibroblast cultures. Scand J Rheumatol 8:173–176
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Vuorio, E., Einola, S., Hakkaramen, S. et al. Synthesis of underpolymerized hyaluronic acid by fibroblasts cultured from rheumatoid and non-rheumatoid synovitis. Rheumatol Int 2, 97–102 (1982). https://doi.org/10.1007/BF00541160
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DOI: https://doi.org/10.1007/BF00541160