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Fractional control of photosynthesis by the QB protein, the cytochrome f/b 6 complex and other components of the photosynthesic apparatus

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Abstract

In order to obtain information on fractional control of photosynthesis by individual catalysts, catalytic activities in photosynthetic electron transport and carbon metabolism were modified by the addition of inhibitors, and the effect on photosynthetic flux was measured using chloroplasts of Spinacia oleracea L. In thylakoids with coupled electron transport, light-limited electron flow to ferricyanide was largely controlled by the QB protein of the electron-transport chain. Fractional control by the cytochrome f/b 6 complex was insignificant under these conditions. Control by the cytochrome f/b 6 complex dominated at high energy fluence rates where the contribution to control of the QB protein was very small. Uncoupling shifted control from the cytochrome f/b 6 complex to the QB protein. Control of electron flow was more complex in assimilating chloroplasts than in thylakoids. The contributions of the cytochrome f/b 6 complex and of the QB protein to control were smaller in intact chloroplasts than in thylakoids. Thus, even though the transit time for an electron through the electron-transport chain may be below 5 ms in leaves, oxidation of plastohydroquinone was only partially responsible for limiting photosynthesis under conditions of light and CO2 saturation. The energy fluence rate influenced control coefficients. Fractional control of photosynthesis by the ATP synthetase, the cytochrome f/b 6 complex and by ribulose-1,5-bisphosphate carboxylase increased with increasing fluence rates, whereas the contributions of the QB protein and of enzymes sensitive to SH-blocking agents decreased. The results show that the burdens of control are borne by several components of the photosynthetic apparatus, and that burdens are shifted as conditions for photosynthesis change.

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Abbreviations

Chl:

chlorophyll

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

DNP-INT:

2,4-dinitro phenylether of 2-iodo-4-nitrothymol

pCMBS:

p-chloromercuribenzosulfonate

References

  • Bendall, D.S. (1982) Photosynthetic cytochromes of oxygenic organisms. Biochim. Biophys. Acta 683, 119–151

    Google Scholar 

  • Buchanan, B.B. (1980) Role of light in the regulation of chloroplast enzymes. Annu. Rev. Plant Physiol. 31, 341–374

    Google Scholar 

  • Dietz, K.-J. (1986) An evaluation of light and CO2 limitation of leaf photosynthesis by CO2 gas exchange analysis. Planta 167, 260–263

    Google Scholar 

  • Dietz, K.-J., Foyer, C. (1986) The relationship between phosphate status and photosynthesis in leaves. Reversibility of the effects of phosphate deficiency on photosynthesis. Planta 167, 376–381

    Google Scholar 

  • Dietz, K.-J., Heber, U. (1984) Rate-limiting factors in leaf photosynthesis. I. Carbon fluxes in the Calvin cycle. Biochim. Biophys. Acta 767, 432–443

    Google Scholar 

  • Dietz, K.-J., Neimanis, S., Heber, U. (1984) Rate-limiting factors in leaf photosynthesis. II. Electron transport. Biochim. Biophys. Acta 767, 444–450

    Google Scholar 

  • Dietz, K.-J., Schreiber, U., Heber, U. (1985) The relationship between the redox state of QA and photosynthesis in leaves at various carbon dioxide, oxygen and light regimes. Planta 166, 219–226

    Google Scholar 

  • Good, N.E., Izawa, S. (1973) Inhibition of photosynthesis. In: Metabolic inhibitors, vol. IV, pp 179–214, Hochster, R.M., Quastel, J.H., eds. Academic Press, New York

    Google Scholar 

  • Hachnel, W. (1984) Photosynthetic electron transport in higher plants. Annu. Rev. Plant Physiol. 35, 659–693

    Google Scholar 

  • Hauska, G., Hurt, E., Gabellini, N., Lockau, W. (1983) Comparative aspects of quinol-cytochrome c/plastocyanin oxidoreductases. Biochim. Biophys. Acta 726, 97–133

    Google Scholar 

  • Heber, U., Boardman, N.K., Anderson, J.M. (1976a) Cytochrome b 563 redox changes in intact CO2-fixing spinach chloroplasts and in developing pea chloroplasts. Biochim. Biophys Acta 423, 275–292

    Google Scholar 

  • Heber, U., Boardman, N.K., Anderson, J.M. (1976b) Effects of pH and oxygen on photosynthetic reactions of intact chloroplasts. Plant Physiol. 57, 277–283

    Google Scholar 

  • Heber, U., Neimanis, S., Dietz, K.-J., Viil, J. (1986) Assimilatory power as a driving force in photosynthesis. Biochim. Biophys. Acta 852, 144–155

    Google Scholar 

  • Heber, U., Santarius, K.A. (1970) Direct and indirect transport of ATP and ADP across the chloroplast envelope. Z. Naturforsch. 25b, 718–728

    Google Scholar 

  • Hill, R., Hartree, E.F. (1953) Hematin compounds in plants. Annu. Rev. Plant Physiol. 4, 115–150

    Google Scholar 

  • Izawa, S., Good, N.E. (1965) The number of sites sensitive to 3-(3,4-dichlorophenyl)-1,1-dimethylurea, 3-(4-chlorophenyl)-1,1-dimethylurea and 2-chloro-4-(2-propylamino)-6-ethylamino-s-triazine in isolated chloroplasts. Biochim. Biophys. Acta 102, 20–38

    Google Scholar 

  • Izawa, S., Winget, G.D., Good, N.E. (1966) Phlorizin, a specific inhibitor of photophosphorylation and phosphorylationcoupled electron transport in chloroplasts. Biochem. Biophys. Res. Comm. 22, 223–226

    Google Scholar 

  • Jensen, R.G., Bassham, J.A. (1966) Photosynthesis by isolated chloroplasts. Proc. Natl. Acad. Sci. USA 56, 1095–1101

    Google Scholar 

  • Kacser, H., Burns, J.A. (1979) Molecular democracy: Who shares the controls? Biochem. Soc. Trans. 7, 1149–1160

    Google Scholar 

  • Laasch, H., Pfister, K., Urbach, W. (1982) High- and lowaffinity binding of photosystem II-herbicides to isolated thylakoid membranes and intact algal cells. Z. Naturforsch. 37c, 620–631

    Google Scholar 

  • Laisk, A., Walker, D.A. (1986) Control of phosphate turnover as a rate-limiting factor and possible cause of oscillations in photosynthesis: A mathematical model. Proc. R. Soc. London, Ser. B 227, 281–302

    Google Scholar 

  • Leegood, R.C., Kobayshi, Y., Neimanis, S., Walker, D.A., Heber, U. (1982) Cooperative activation of chloroplast fructose-1,6-bisphosphatase by reductant, pH and substrate. Biochim. Biophys. Acta 682, 168–178

    Google Scholar 

  • Li, Y.S., Gibbs, M. (1985) Pyridoxal phosphate and 3-phosphoglycerate overcome the D,l-glyceraldehyde inhibition of carbon dioxide fixation by intact chloroplasts. Plant Physiol. 77, 42

    Google Scholar 

  • Madsen, N.B. (1963) Mercaptide forming agents. In Metabolic inhibitors, vol 2, pp. 119–143, Hochster, R.M., Quastel, J.H., eds. Academic Press, New York

    Google Scholar 

  • McCarty, R.E. (1980) Deineation of the mechanism of ATP synthesis in chloroplasts: use of uncoplers, energy transfer inhibitors, and modifiers of coupling factor I. Methods Enzymol. 69, 719–728

    Google Scholar 

  • Ohmori, M., Gimmler, H., Schreiber, U., Heber, U. (1985) Relative insensitivity of photosynthesis to the dissipation of a transthylakoid proton gradient in intact chloroplasts. Physiol. Vég. 23, 801–812

    Google Scholar 

  • Sharkey, T.D., Stitt, M., Heinecke, D., Gerhardt, R., Raschke, K., Heldt, H.W. (1986) Limitation of photosynthesis by carbon metabolism. II. Oxygen insensitive CO2 uptake results from limitation of triosephosphate utilization. Plant Physiol. 81, 1123–1129

    Google Scholar 

  • Sivak, M.N., Walker, D.A. (1986) Photosynthesis in vivo can be limited by phosphate supply. New Phytol. 102, 499–512

    Google Scholar 

  • Slabas, A.R., Walker, D.A. (1978) Inhibition of spinach phosphoribulokinase by D,l-glyceraldehyde. Biochem. J. 153, 613–619

    Google Scholar 

  • Stitt, M. (1986) Limitation of photosynthesis by carbon metabolism. I. Evidence for excess electron transport capacity in leaves carrying out photosynthesis in saturating light and CO2. Plant Physiol. 81, 1115–1122

    Google Scholar 

  • Stitt, M. (1987) Limitation of photosynthesis by sucrose synthesis. In: Progress in photosynthesis research, vol. 3, pp. 685–692, Biggins, J., ed. Martinus Nijhoff Publishers, Dordrecht Boston Lancaster

    Google Scholar 

  • Takahama, U., Shimidzu-Takahama, M., Heber, U. (1981) The redox state of the NADP system in illuminated chloroplasts. Biochim. Biophys. Acta 637, 530–539

    Google Scholar 

  • Trebst, A. (1980) Inhibitors in electron flow: Tools for the functional and structural localization of carriers and energy conservation sites. Methods Enzymol. 69, 675–715

    Google Scholar 

  • Trebst, A., Wietoska, H., Draber, W., Knops, H.J. (1978) The inhibition of photosynthetic electron flow in chloroplasts by the dinitrophenylether of bromo-or iodo-nitrothymol. Z. Naturforsch. 33c, 919–927

    Google Scholar 

  • von Caemmerer, S., Farquhar, G.D. (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta 153, 376–387

    Google Scholar 

  • Witt, H.T. (1979) Energy conversion in the functional membrane of photosynthesis. Analysis by light pulse and electric pulse methods. Biochim. Biophys. Acta 505, 355–427

    Google Scholar 

  • Wishnick, M., Lane, M.D. (1971) Ribulose diphosphate carboxylase from spinach leaves. Methods Enzymol. 23, 570–577

    Google Scholar 

  • Woodrow, I.E. (1986) Control of the rate of photosynthetic carbon dioxide fixation. Biochim. Biophys. Acta 851, 181–192

    Google Scholar 

  • Woodrow, I.E., Ball, J.T., Berry, J.A. (1986) A general expression for the control of the rate of photosynthetic CO2 fixation by stomata, the boundary layer and radiation exchange. In: Progress in photosynthesis research, vol. 4, pp. 225–228, Biggins, J. ed. Martinus Nijhoff Publishers, Dordrecht Boston Lancaster

    Google Scholar 

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Authors and Affiliations

  1. Institute of Botany and Pharmaceutical Biology of the University, Mittlerer Dallenbergweg 64, D-8700, Würzburg, Germany

    U. Heber, S. Neimanis & K. -J. Dietz

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  1. U. Heber
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  2. S. Neimanis
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  3. K. -J. Dietz
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Heber, U., Neimanis, S. & Dietz, K.J. Fractional control of photosynthesis by the QB protein, the cytochrome f/b 6 complex and other components of the photosynthesic apparatus. Planta 173, 267–274 (1988). https://doi.org/10.1007/BF00403020

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  • DOI: https://doi.org/10.1007/BF00403020

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