Abstract
Native-state organization and distribution of cell-wall components in the secondary wall of woody tissue from P. mariana (Black Spruce) have been investigated using polarized Raman microspectroscopy. Evidence for orientation is detected through Raman intensity variations resulting from rotations of the exciting electric vector with respect to cell-wall geometry. Spectral features associated with cellulose and lignin were studied. The changes in cellulose bands indicate that the pyranose rings of the anhydroglucose repeat units are in planes perpendicular to the cross section, while methine C−H bonds are in planes parallel to the cross section. Changes in bands associated with lignin indicate that the aromatic rings of the phenyl-propane units are most often in the plane of the cell-wall surface. However, regions where lignin orientation departs from this pattern also occur. These results represent direct evidence of molecular organization with respect to cellular morphological features in woody tissue, and indicate that cell-wall components are more highly organized than had been recognized. Studies carried out in order to establish the usefulness and sensitivity of the Raman technique to differences of composition within the cell walls provide evidence of variations in the distribution of cellulose and lignin. Such compositional differences were more prominent between the walls of different cells than within a particular cell wall.
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References
Andersen, M.E., Muggli, R.Z. (1981) Microscopical techniques with the molecular optics laser examiner. Anal. Chem. 53, 1772–1777
Atalla, R.H. (1976) Raman spectral studies of polymorphy in cellulose. I. Celluloses I and II. Appl. Polym. Symp. 28, 659–669
Atalla, R.H., Agarwal, U.P. (1984) Raman microprobe optimization, and sampling technique for studies of plant cell walls. In: Microbeam analysis, pp. 125–126, Romig, A.D., Goldstein, J.I., eds. San Francisco Press, San Francisco, Cal
Atalla, R.H., Agarwal, U.P. (1985) Raman microprobe evidence for lignin orientation in the cell walls of native woody tissue. Science 227, 636–638
Atalla, R.H., Whitmore, R.E., Heimbach, C.J. (1980) Raman spectral evidence for molecular orientation in native cellulosic fibers. Macromolecules 13, 1717–1719
Browning, B.L. (1967) Methods of wood chemistry. Interscience, New York
Ehrhardt, S.M. (1986) The vibrational spectra of lignin model compounds. Doct. dissert., Institute of Paper Chemistry, Appleton, Wis., USA
French, A.D. (1978) The crystal structure of native ramie cellulose. Carbohydr. Res. 61, 67–80
Frey-Wyssling, A. (1976) The plant cell wall. Gebrüder Bornträger, Berlin
Gilson, T.R., Hendra, P.J. (1970) Laser Raman spectroscopy. Wiley, London
Harada, H., Goto, T. (1982) The structure of cellulose microfibrils in Valonia. In: Cellulose and other natural polymer systems, pp. 383–401, Brown, M.R., Jr., ed. Plenum Press, New York
Hardell, H.L., Westermark, U. (1981) The carbohydrate composition of the outer cell walls of spruce fibers. Int. Symp. on Wood and Pulping Chemistry, Stockholm (SPCI Rep. No. 38), vol. 1, pp. 32–34
Harrand, M. (1983) Polarized Raman spectra of oriented dipalmitoylphosphatidylcholine (DPPC). I. Scattering activities of skeletal stretching and methylene vibrations of hydrocarbon chains. J. Chem. Phys. 79, 5639–5651
Marchessault, R.H., Liang, C.Y. (1962) The infrared spectra of crystalline polysaccharides. VIII. Xylans. J. Polym. Sci. 59, 357–378
Meier, H. (1961) The distribution of polysaccharides in wood fibers. J. Polym. Sci. 51, 11–18
Page, D.H., El-Hosseniy, E., Bidmade, M.L., Binet, R. (1976) Birefringence and the chemical composition of wood pulp fibers. Appl. Polym. Symp. 28, 923–929
Preston, R.D. (1974) The physical biology of plant cell walls. Chapman & Hall, London
Revol, J.-F., Gancet, C., Goring, D.A.I. (1982) Orientation of cellulose crystallite in S2 layer of spruce and birchwood cell walls. Wood Sci. 14, 120–126
Rosasco, G.J. (1980) Raman microprobe spectroscopy. In: Advances in infrared and Raman spectroscopy, Vol. 7, pp. 223–282, Clark, R.J.H., Hester, R.E., eds. Heyden & Sons, London
Schaefer, J., Sefcik, M.D., Stejskal, E.O., McKay, R.A., Hall, P.L. (1981) Characterization of the catabolic transformation of lignin culture using magic angle carbon-13 nuclear magnetic resonance. Macromolecules 14, 557–559
Simon, B.W., Timell, T.E. (1978) Polysaccharides in cambial tissue of Populus tremuloides and Tilia americana I. Isolation, fractionation, and chemical composition of the cambial tissues. Cellul. Chem. Technol. 12, 39–50
Wells, H.A. (1977) An investigation of the vibrational spectra of glucose, galactose and mannose. Doct. dissert., Institute of Paper Chemistry, Appleton, Wis., USA. Dissert. Abstr. Intl. 39, 4919-B (1979)
Whiting, P., Favis, B.D., St.-Germain, F.G.T., Goring, D.A.I. (1981) Fractional separation of middle lamella and secondary wall tissue from spruce wood. J. Wood Chem. Technol. 1, 29–42
Whiting, P., Goring, D.A.I. (1983) The composition of the carbohydrates in the middle lamella and secondary wall of tracheids from black spruce wood. Can. J. Chem. 61, 506–508
Wiley, J., Atalla, R.H. (1986) Raman spectra of cellulose. In: Am. Chem. Soc. Symp. Ser. “Solid State Characterization of Cellulose” (in press)
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Agarwal, U.P., Atalla, R.H. In-situ Raman microprobe studies of plant cell walls: Macromolecular organization and compositional variability in the secondary wall of Picea mariana (Mill.) B.S.P.. Planta 169, 325–332 (1986). https://doi.org/10.1007/BF00392127
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DOI: https://doi.org/10.1007/BF00392127