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Chloroplast DNA from the fern Osmunda cinnamomea: physical organization, gene localization and comparison to angiosperm

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Summary

Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was constructed by filter hybridizations using purified PvuII fragments as hybridization probes. The Osmunda chloroplast genome is circular and contains an inverted repeat 8–13 kb in size.

Gene probes from tobacco, corn and spinach were used to map the positions of six genes on the Osmunda chloroplast chromosome. The 16S and 23S ribosomal RNAs are encoded by duplicate genes which lie within the inverted repeat. Genes for the large subunit of ribulose-1,5-bisphosphate carboxylase, a photosystem II polypeptide, and the alpha and beta subunits of chloroplast coupling factor are located in three different segments of the large single copy region.

The Osmunda chloroplast genome is remarkably similar in size, conformation, physical organization, and map positions of known genes, to chloroplast DNA from a number of angiosperms. The major difference between chloroplast DNA from this fern and angiosperms is that the inverted repeat is smaller in Osmunda (8–13 kb) than in angiosperms (22–25 kb).

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Abbreviations

PEG:

polyethylene glycol 4000

kb:

kilobase pairs

bp:

base pairs

rRNA:

ribosomal RNA

LS:

large subunit of ribulose-1,5-bisphosphate carboxylase

PII:

32,000 dalton photosystem II polypeptide (Mattoo et al. 1981)

CFα :

alpha subunit of chloroplast coupling factor

CF β :

beta subunit of chloroplast coupling factor

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Authors and Affiliations

  1. Department of Plant Biology, Carnegie Institution of Washington, 94305, Stanford, CA, USA

    Jeffrey D. Palmer

  2. Department of Biological Sciences, Mount Holyoke College, 01075, South Hadley, MA, USA

    Diana B. Stein

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  1. Jeffrey D. Palmer
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  2. Diana B. Stein
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Palmer, J.D., Stein, D.B. Chloroplast DNA from the fern Osmunda cinnamomea: physical organization, gene localization and comparison to angiosperm. Curr Genet 5, 165–170 (1982). https://doi.org/10.1007/BF00391801

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  • DOI: https://doi.org/10.1007/BF00391801

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