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UV and chemical mutagenesis in rev7 mutants of yeast

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Summary

We have examined induced mutagenesis in rev7-1 mutants of Baker's yeast' Saccharomyces cerevisiae, using a variety of contrasting test systems and several different mutagens. UV-induced reversion frequencies of the ochre allele arg4-17, the putative missense allele ilv1-92 and the frameshift allele his4-38 were 10 to 200 fold lower in haploid and diploid rev7-1 mutants compared with wild type strains, but UV-induced reversion frequencies of the frameshift allele leu2-3 and the proline missense allele cyc1-115 were reduced only a few fold. Ilv1-92 reversion frequencies induced by methyl methane sulfonate or by N-methyl-N′-nitro-N-nitrosoguanidine were 10 to 20 times lower in rev7-1 mutants, but normal frequencies of these revertants were induced with ethyl methane sulfonate, even though rev7-1 strains are slightly sensitive to this mutagen as well as to the others tested. We conclude that the rev7 mutants, like the rev3 mutants they closely resemble, have a substantial but not total deficiency concerning induced mutagenesis.

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Authors and Affiliations

  1. Department of Radiation Biology and Biophysics, University of Rochester, School of Medicine and Dentistry, 14642, Rochester, NY, USA

    Christopher W. Lawrence, Paul E. Nisson & Roshan B. Christensen

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  1. Christopher W. Lawrence
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  2. Paul E. Nisson
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  3. Roshan B. Christensen
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Communicated by C. Auerbach

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Lawrence, C.W., Nisson, P.E. & Christensen, R.B. UV and chemical mutagenesis in rev7 mutants of yeast. Molec. Gen. Genet. 200, 86–91 (1985). https://doi.org/10.1007/BF00383317

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  • DOI: https://doi.org/10.1007/BF00383317

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