Abstract
In situ hybridization of labeled DNA of four mobile dispersed genetic elements (mdg), isolated from D. melanogaster and C. virilis genomes, with polytene chromosomes of the larvae of several Drosophila species has been carried out. The data show that the mdg elements exhibit a high degree of species specificity. The same conclusions are derived from filter hybridization using 32P-labeled D. melanogaster and D. virilis DNA and cloned mdg sequences immobilized on nitrocellulose filters. We attempted to induce transpositions (“jumping”) of mdg elements specific for D. virilis chromosomes to the chromosomes of related species (e.g. D. littoralis Meigen) originally lacking the representatives of this family of repeats. For this purpose we produced hybrid stocks with “synthetic” karyotoypes characterized by different combinations of D. virilis homologous chromosomes and “hybrid” chromosomes. In one of such stocks we did find by in situ hybridization the insertion of a D. virilis mdg element into the fifth chromosome of D. littoralis Meigen. The transposition (“jumping”) took place in the only region where somatic pairing between the fifth chromosomes of D. virilis and D. littoralis occurs more or less regularly in the hybrids. Since crossing-over in hybrid chromosomes of males is excluded in such “synthetic” stocks, gene conversion may be responsible for this transposition. The possible bearing of the phenomenon observed on the problem of hybrid dysgenesis is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ananiev, E.V., Gvozdev, V.A., Ilyin, Y.V., Tchurikov, N.A., Georgiev, G.P.: Reiterated genes with varying location in intercalary heterochromatin regions of Drosophila melanogaster polytene chromosomes. Chromosoma (Berl.) 70, 1–17 (1978)
Birnboim, H.C., Doly, J.: A rapid alkaline extraction procedure for screening recombinant plasmit DNA. Nucleic Acids Res. 7, 1513–1523 (1979)
Bolivar, F., Rodriguez, R.C., Greene, P.J., Betlach, M.C., Heyneker, H.C., Boyer, H.W., Crosa, J.H., Falkow, S.: Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2, 95–113 (1977)
Bonner, J.J., Pardue, M.L.: Ecdysone-stimulated RNA synthesis in imaginal discs of Drosophila melanogaster. Chromosoma (Berl.) 58, 87–99 (1976)
Bregliano, J.C., Picard, L., Bucheton, A., Relisson, A., Lavige, J.M., L'Heriter, P.: Hybrid dysgenesis in D. melanogaster. Science 207, 610–611 (1980)
Brown, A.J.H., Ish-Horowicz, D.: Evolution of the 87 C heat-shock loci in Drosophila. Nature (Lond.) 290, 677–680 (1981)
Calos, M.P., Miller, J.H.: Transposable elements. Cell 20, 579–596 (1980)
Cameron, J.R., Loh, E.Y., Davis, R.W.: Evidence for transposition of dispersed repetitive DNA families in yeast. Cell 16, 739–752 (1979)
Cami, B., Kourilsky, P.: Screening of cloned recombinant DNA in bacteria by in situ colony hybridization. Nucleic Acids Res. 5, 2381–2390 (1978)
Dunsmuir, P., Brorein, W.J., Simon, M.A., Rubin, G.M.: Insertion of the Drosophila transposable element copia generates a 5 base pair duplication. Cell 21, 275–580 (1980)
Evgen'ev, M.B., Kolchinski, A., Levin, A., Preobrazhenskaya, O., Sarkisova, E.: Heat shock DNA homology in distantly related species of Drosophila. Chromosoma (Berl.) 68, 357–365 (1978)
Finnegan, D.J., Rubin, G.M., Young, H.W., Hogness, D.S.: Repeated genefamilies in Drosophila melanogaster. Cold Spr. Harb. Symp. quant. Biol. 42, 1053–1064 (1977)
Flavell, A.J., Ish-Horowicz, D.: Extrachromosomal circular copies of the eucaryotic transposable element copia in cultured Drosophila cells. Nature (Lond.) 292, 591–594 (1981)
Gatner, J., Philippsen, P.: The yeast transposon Tyl generates duplications of target DNA on insertion. Nature (Lond.) 286, 414–418 (1980)
Ilyin, Y.V., Tchurikov, N.A., Georgiev, G.P.: Selection and some properties of recombinant clones of lambda bacteriophage containing structural genes of Drosophila melanogaster. Nucleic Acids Res. 3, 2115–2127 (1976)
Ilyin, Y.V., Tchurikov, N.A., Ananiev, E.V., Ryskov, A.P., Yenikolopov, G.N., Limborska, S.A., Maleeva, N.E., Gvozdev, V.A., Georgiev, G.P.: Studies on the DNA fragments of mammals and Drosophila containing structural genes and adjacent sequences. Cold Spr. Harb. Symp. quant. Biol. 42, 959–970 (1977)
Ilyin, Y.V., Chmeliauskaite, V.G., Georgiev, G.P.: Double-stranded sequences in RNA of Drosophila melanogaster: relation to mobile-dispersed genes. Nucleic Acids Res. 8, 3439–3457 (1980a)
Ilyin, Y.V., Chmeliauskaite, V.G., Ananiev, E.V., Georgiev, G.P.: Characterization of a new family of mobile dispersed genetic element mdg3 in D. melanogaster. Chromosoma (Berl.) 81, 27–54 (1980b)
Kleckner, N.: Transposable elements in procaryotes. Cell 11, 11–23 (1977)
Orgel, L.E., Crick, F.H.C.: Selfish DNA: the ultimate parasite. Nature (Lond.) 284, 604–607 (1980)
Pardue, M.L., Kedes, L.H., Weinberg, E.S., Birnstiel, M.L.: Localization of sequences coding for histone messenger RNA in the chromosomes of D. melanogaster. Chromosoma (Berl.) 63, 135–151 (1977)
Patterson, J.T., Stone, W.S.: Evolution in the Genus Drosophila. New York: Maxmillan 1952
Pierce, D.A., Lucchesi, J.C.: Analysis of a dispersed repeat DNA sequence in isogenic lines of Drosophila. Chromosoma (Berl.) 82, 471–493 (1981)
Potter, S., Truett, M., Phillips, M., Maher, A.: Eucaryotic transposable genetic elements with inverted terminal repeats. Cell 20, 639–648 (1980)
Rigby, P.W.S., Diekmann, M., Rhodes, C., Berg, P.: Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Molec. Biol. 113, 237–251 (1977)
Scherer, S., Davis, R.W.: Recombination dispersed repeated DNA sequences in yeast. Science 209, 1380–1384 (1980)
Shaw, J.E., Huang, E.-S., Pagano, J.S.: Iodination of herpes virus nucleic acids. J. Virology 16, 132–140 (1975)
Shoemaker, C., Goff, S., Gilboa, E., Paskind, M., Mitra, S.M., Baltimore, D.: Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment: Implications for retrovirus integration. Proc. nat. Acad. Sci. (Wash.) 77, 3932–3936 (1980)
Strobel, S.W., Dunsmuir, P., Rubin, G.M.: Polymorphism in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell 17, 429–439 (1979)
Tchurikov, N.A., Zelentsova, E.S., Georgiev, G.P.: Clusters containing different mobile dispersed genes in the genome of D. melanogaster. Nucleic Acids Res. 8, 1243–1258 (1980)
Young, M.W.: Middle repetitive DNA: a fluid component of the Drosophila genome. Proc. Natl. Acad. Sci. (Wash.) 76, 6274–6278 (1979)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evgen'ev, M.B., Yenikolopov, G.N., Peunova, N.I. et al. Transposition of mobile genetic elements in interspecific hybrids of Drosophila . Chromosoma 85, 375–386 (1982). https://doi.org/10.1007/BF00330360
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330360