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Production of cis,cis-muconate from benzoate and 2-fluoro-cis,cis-muconate from 3-fluorobenzoate by 3-chlorobenzoate degrading bacteria

  • Applied Microbiology
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Summary

3-Chlorobenzoate grown cells of Pseudomonas sp. strain B13 or Alcaligenes sp. strain A7-2 converted 3-fluorobenzoate to 2-fluoro-cis,cis-muconate with 87% yield. The latter strain produced 1.6 g/l. The type II muconate cycloisomerases of neither strain exhibit acitivity for 2-fluoro-cis,cis-muconate. Succinate grown cells of Pseudomonas sp. strain B13 converted benzoate to cis,cis-muconate (91% yield; 7.4 g/l). Enzyme tests confirmed that no muconate cycloisomerising enzyme was induced within 24 h.

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Authors and Affiliations

  1. Gesamthochschule Wuppertal, Lehrstuhl für Chemische Mikrobiologie der Bergischen Universität, Gaußstr. 20, D-5600, Wuppertal, Germany

    Eberhard Schmidt & Hans -Joachim Knackmuss

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  1. Eberhard Schmidt
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  2. Hans -Joachim Knackmuss
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Schmidt, E., Knackmuss, H.J. Production of cis,cis-muconate from benzoate and 2-fluoro-cis,cis-muconate from 3-fluorobenzoate by 3-chlorobenzoate degrading bacteria. Appl Microbiol Biotechnol 20, 351–355 (1984). https://doi.org/10.1007/BF00270599

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  • DOI: https://doi.org/10.1007/BF00270599

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