Summary
A slow growing mutant of Paramecium cl-1, deficient in type-a cytochrome is described: it differs from wild type by a recessive nuclear mutation and also by its mitochondria. Mitochondria of the mutant are “compatible” with gene cl-1, whereas mitochondria of the wild type are “incompatible” with this gene: they are spectacularly altered in the presence of a cl-1/cl-1 nucleus. However this incompatibility is temporary and mitochondria of wild type origin slowly become compatible with cl-1. The molecular basis of the incompatibility and the mechanism of its disappearance are discussed.
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Adoutte, A., Balméfrézol, M., Beisson, J., André, J.: The effects of erythromycin and chloramphenicol on the ultrastructure of mitochondria in sensitive and resistant strains of Paramecium. J. Cell. Biol. 54, 8 (1972)
Adoutte, A., Beisson, J.: Evolution of mixed populations of genetically different mitochondria. Nature (Lond.) 235, 393 (1972)
Avner, P. R., Griffiths, D. E.: Studies on energy-linked reactions. Genetic analysis of oligomycin-resistant mutants of Saccharomyces cerevisiae. Europ. J. Biochem. 32, 312 (1973)
Beisson, J., Beale, G. H., Knowles, J., Sainsard, A., Adoutte, A., Tait, A.,: Genetic control of mitochondria in Paramecium. Genetics (in press)
Beisson, J., Rossignol, M.: The first case of linkage in Paramecium aurelia. Genet. Res. 13, 85 (1969)
Beisson, J., Sonneborn, T. M.: Cytoplasm inheritance of the organization of the cell cortex in Paramecium aurelia. Proc. nat. Acad. Sci. (Wash.) 53, 275 (1965)
Boardman, N., Linnane, A., Smillie, R., editors: Autonomy and biogenesis of mitochondria and chloroplasts. Amsterdam: North Holland Publishing Co. 1971
Charret, R., Fauré-Fremiet, E.: Technique de rassemblement de microorganismes: préinclusion dans un caillot de fibrine. J. Microsc. 6, 1063 (1967)
Claisse, M., Péré-Aubert, G., Clavillier, L., Slonimski, P.: Méthode d'estimation de la concentration des cytochromes dans les cellules entières de levure. Europ. J. Biochem. 16, 430 (1970)
Davies: Cell wall organization in Chlamydomonas reinhardi. The role of extra nuclear systems. Molec. Gen. Genet. 115, 334 (1972)
Edwards, D. L., Kwiecinski, F.: Altered mitochondrial respiration in a chromosomal mutant of Neurospora crassa. J. gen. Microbiol. 116, 610 (1973)
Jagow, G. von, Weiss, H., Klingenberg, M.: Comparison of the respiratory chain of Neurospora crassa wild type and the mi-mutants, mi-1 and mi-3. Europ. J. Biochem. 33, 140 (1973)
Knowles, J.: A study of cytoplasmic erythromycin resistance in Paramecium aurelia using an improved microinjection technique. J. Cell Sci. (in press)
Kung, C.: The electron transport system of kappa particles from Paramecium aurelia, Stock 51. J. gen. Microbiol. 61, 371 (1970)
Lambowitz, A. M., Smith, E.W., Slayman, C.W.: Electron transport in Neurospora mitochondria. Studies on wild type and poky. J. biol. Chem. 247, 4850 (1972)
Lambowitz, A. M., Smith, E. W., Slayman, C. W.: Oxidative phosphorylation in Neurospora mitochondria. Studies on wild type, poky, and chloramphenicol induced wild type. J. biol. Chem. 247, 4859 (1972)
Luft, J.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409 (1961)
Mason, T., Schatz, G.: Cytochrome c oxidase from baker's yeast. II. Site of translation of the protein components. J. biol. Chem. 248, 1355 (1973)
Rabinowitz, M., Swift, H.: Mitochondrial nucleic acids and their relation to the biogenesis of mitochondria. Physiol. Rev. 50, 376 (1970)
Schotz, F.: Effects of the disharmony between genome and plastome on the differentiation of the thylakoid system in Oneothera in Symposia of the society for Experimental Biology, 24, Control of organelle development, p. 39. London: Camb. Univ. Press 1970
Sebald, W., Weiss, H., Jackl, G.: Inhibition of the assembly of cytochrome oxidase in Neurospora crassa by chloramphenicol. Europ. J. Biochem. 30, 413 (1972)
Sherman, F., Slonimski, P.: Respiration-deficient mutants of yeast. II. Biochemistry. Biochim. biophys. Acta (Amst.) 90, 1–15 (1964)
Smith, M. H., Georges, P., Preer, J. R.: Preliminary observations on isolated Paramecium hemoglobin. Arch. Biochem. Biophys. 99, 313 (1962)
Sonneborn, T. M.: Patterns of nucleocytoplasmic integration in Paramecium. Caryologia 6 (Suppl.) (1954)
Sonneborn, T. M.: In the nature of biological diversity (ed. J. M. Allen). New York: McGraw Hill 1963
Sonneborn, T. M.: Methods in Paramecium research. In: Methods in cell physiology 4, 241 (1970)
Stickland, L. H.: The determination of small quantities of bacteria by means of the Biuret reaction. J. gen. Microbiol. 5, 698 (1951)
Tzagoloff, A., Meagher, P.: Assembly of the mitochondrial membrane system. VI. Mitochondrial synthesis of subunit proteins of the rutamycin-sensitive adenosine triphosphatase. J. biol. Chem. 247, 594 (1972)
Weiss, H., Sebald, W., Bucher, Th.: Cycloheximide resistant incorporation of aminoacids into a polypeptide of the cytochrome oxidase of Neurospora crassa. Europ. J. Biochem. 22, 19 (1971)
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Sainsard, A., Claisse, M. & Balméfrézol, M. A nuclear mutation affecting structure and function of mitochondria in Paramecium . Molec. Gen. Genet. 130, 113–125 (1974). https://doi.org/10.1007/BF00269083
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DOI: https://doi.org/10.1007/BF00269083