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Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region

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Abstract

We previously reported the presence of a new gene (HSET) with an unknown function, in the centromeric side of the class II gene region of the human major histocompatibility complex (MHC). cDNA clones corresponding to the HSET gene were isolated from a human testis cDNA library. A 2.4 kilobase transcript from the HSET gene was abundantly expressed in testis, B-cell, T-cell, and ovary cell lines but was not detected in lung or stomach. Analysis of the nucleotide sequence of the HSET cDNA clones revealed significant similarity to kinesin-related proteins in yeast, Drosophila, and human. Its predicted amino acid sequence contains a domain with strong sequence similarity to the ATP-binding and motor domains of a plus end-directed microtuble motor protein, kinesin, which might be involved in mitotic chromosome segregation, suggesting that the HSET gene encodes a novel kinesin-related protein.

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References

  • Abe, K., Wei, J. F., Wei, F. S., Hsu, Y. C., Uehara, H., Artzt, D., and Bennet, D. Searching for coding sequence in the mammalian genome: the H-2K region of the mouse MHC is replete with genes expressed in embryos. EMBO J 7: 3441–3449, 1988

    Google Scholar 

  • Campbell, R. D. and Trowsdale, J. Map of the human MHC. Immunol Today 14: 349–352, 1993

    Article  CAS  PubMed  Google Scholar 

  • Geraghty, D. E., Pei, J., Lipsky, B., Hansen, J. A., Taillon-Miller, P., Bronson, S. K., and Chaplin, D. D. Cloning and physical mapping of the HLA class I region spanning the HLA-E- to HLA-F interval by using yeast artificial chromosomes. Proc Natl Acad Sci USA 89: 2669–2673, 1992

    Google Scholar 

  • Glynne, R., Powis, S. H., Beck, S., Kelly, A., Kerr, L. A., and Trowsdale, J. A proteasome-related gene between the two ABC transporter loci in the class II region of the human MHC. Nature 353: 357–360, 1991

    Google Scholar 

  • Hanson, I. M. and Trowsdale, J. Colinearity of novel genes in the class II region of the MHC in mouse and man. Immunogenetics 34: 5–11, 1991

    Google Scholar 

  • Imai, T. and Olson, M. V. Second-generation approach to the construction of yeast artificial-chromosome libraries. Genomics 8: 297–303, 1990

    Google Scholar 

  • Inoko, H., Ando, A., Ito, M., and Tsuji, K. Southern hybridization analysis of DNA polymorphism in the HLA-D region. Hum Immunol 16: 304–313, 1986

    Google Scholar 

  • Janatipour, M., Naumov, Y., Ando, A., Sugimura, K., Tsuji, K., Abe, K., and Inoko, H. Search for MHC-associated genes in human: five new genes centromeric to HLA-DP with yet unknown functions. Immunogenetics 35: 272–278, 1992

    Google Scholar 

  • Kappes, D. and Strominger, J. L. Human class II major histocompatibility complex genes and proteins. Annu Rev Biochem 57: 991–1028, 1988

    Google Scholar 

  • Kelly, A., Monaco, J., Cho, S., and Trowsdale, J. A new human HLA class II-related locus, DM. Nature 353: 571–572, 1991

    Article  CAS  PubMed  Google Scholar 

  • Matsumoto, K., Arai, M., Ishihara, N., Ando, A., Inoko, H., and Ikemura, T. Cluster of fibronectin type III repeats found in the human major histocompatibility complex class III region shows the highest homology with the repeats in an extracellular matrix protein, tenascin. Genomics 12: 485–491, 1992

    Google Scholar 

  • McDonald, H. B. and Goldstein, S. B. Identification and characterization of a gene encoding a kinesin-like protein in Drosophila. Cell 61: 991–1000, 1990

    Google Scholar 

  • McLachlan, A. D. and Karn, J. Peptide features in the amino acid sequence of nematode myosin rod. J Mol Biol 164: 605–626, 1983

    Google Scholar 

  • Meluh, P. B. and Rose, M. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell 60: 1029–1041, 1990

    Google Scholar 

  • Navone, F., Niclas, J., Hom-Booher, N., Spark, L., Bernstein, H., McCaffrey, G. and Vle, D. Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells. J Cell Biol 117: 1263–1275, 1992

    Google Scholar 

  • Nislow, C., Lombillo, V. A., Kuriyama, R., and McIntosh, J. R. A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles. Nature 359: 543–547, 1992

    Google Scholar 

  • Okamoto, N., Ando, A., Kawai, J., Yoshiwara, T., Tsuji, K., and Inoko, H. Orientation of HLA-DNA gene and identification of CpG island-associated gene adjacent to DNA in human major histocompatibility complex class II region. Hum Immunol 32: 221–228, 1991

    Google Scholar 

  • Sambrook, J., Fritsch, E. F., and Maniatis, T. Isolation of total RNA by extraction with strong denaturation. Molecular Cloning: A Laboratory Manual, pp. 7.19–7.22, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1989

    Google Scholar 

  • Sargent, C. A., Dunham, I., Trowsdale, J., and Campbell, R. D. Human major histocompatibility complex contains genes for the major heat shock protein HSP70. Proc Natl Acad Sci USA 86: 1968–1972, 1989

    Google Scholar 

  • Smith, C. L. and Canter, C. Purification, specific fragmentation, and separation of large DNA molecules. Methods Enzymol 155: 449–467, 1987

    Google Scholar 

  • Spies, T., Bresnahan, M., Bahram, S. A., Arnold, D., Blanck, G., Mellins, E., Pious, D., and DeMars, R. A gene in the major histocompatibility complex class II region containing the class I antigen presentation pathway. Nature 348: 744–747, 1990

    Google Scholar 

  • Springer, T. A. Adhesion receptors of the immune system. Nature 346: 425–434, 1990

    Google Scholar 

  • Svejgaard, A., Platz, P., and Ryder, L. P. HLA and disease 1982- a survey. Immunol Rev 70: 193–218, 1983

    Google Scholar 

  • Trowsdale, J., Hanson, I., Mockridge, I., Beck, S., Townsend, A. and Kelly, A. Sequences encoded in the class II region of the MHC related to the ‘ABC’ superfamily of transporters. Nature 348: 741–744, 1990

    Google Scholar 

  • Yen, T. J., Li, G., Schaar, B. T., Szilak, I., and Cleveland, D. W. CENP-E is a putative kinetochore motor that accumulates just before mitosis. Nature, 359: 536–539, 1992

    Google Scholar 

  • Yeom, Y., Abe, K., Bennett, D., and Artz, K. Testis-/embryo-expressed genes are clustered in the mouse H-2K region. Proc Natl Acad Sci USA 89: 773–777, 1992

    Google Scholar 

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Correspondence to: A. Ando.

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Ando, A., Kikuti, Y.Y., Kawata, H. et al. Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region. Immunogenetics 39, 194–200 (1994). https://doi.org/10.1007/BF00241260

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  • DOI: https://doi.org/10.1007/BF00241260

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