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Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene

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Abstract

Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R1 plants were also demonstrated. About 30% of R2 plants derived from one transgenic line showed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.

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Abbreviations

BAP:

6-benzyladenine phosphate

BPMV:

bean pod mottle virus

CP-P:

coat protein-precursor

CTAB:

hexadecyltrimethylammonium bromide

DAS-ELISA:

double antibody sandwich-enzyme-linked immunosorbent assay

IBA:

indole-butyric acid

kbp:

kilobase pairs

MES:

2-(N-Morpholino)ethanesulfonic acid

NOS:

nopaline synthase

NPTII:

neomycin phosphotransferase II

NTP:

nucleoside triphosphate

PBS:

phosphate-buffered saline

PCR:

polymerase chain reaction

PVP:

polyvinyl pyrrolidone

VPg:

viral genome-linked protein

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Authors and Affiliations

  1. Department of Plant Pathology, University of Kentucky, 40546, Lexington, KY, USA

    Rong Di, Virginia Purcell & Said A. Ghabrial

  2. Department of Agronomy, University of Kentucky, 40546, Lexington, KY, USA

    Glenn B. Collins

Authors
  1. Rong Di
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  2. Virginia Purcell
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  3. Glenn B. Collins
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  4. Said A. Ghabrial
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Additional information

Communicated by W. A. Parrott

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Di, R., Purcell, V., Collins, G.B. et al. Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene. Plant Cell Reports 15, 746–750 (1996). https://doi.org/10.1007/BF00232220

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  • DOI: https://doi.org/10.1007/BF00232220

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