Skip to main content
SpringerLink
Log in

Cross-circulation studies on the influence of hypoxia and hypoxaemia on neuro-epithelial bodies in young rabbits

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The reactions of the previously described neuro-epithelial bodies (NEB) (Lauweryns et al., 1969, 1970, 1972a, b, 1973a, b, c, 1974, 1975) in young rabbits to: (1) hypoxia with normoxaemia in the arteria pulmonalis on the one hand, and (2) hypoxaemia in the arteria pulmonalis with normoxic aeration on the other hand, has been investigated by means of cross-circulation experiments and light microscopical, electron microscopical and morphometrical techniques.

Hypoxically aerated young rabbits, which received normoxaemic blood in their arteria pulmonalis from a donor rabbit by means of an arterio-arterial cross-circulation with mutual exchange transfusion, revealed an increased exocytosis of the dense-core vesicles of their NEB. Normoxically aerated young rabbits which received hypoxaemic blood in an identical manner, did not exhibit an increased exocytosis.

It is concluded that the NEB apparently react directly to the hypoxia of the inhaled air and not to the hypoxaemia of the pulmonary blood. By the release of serotonin and a polypeptide substance, they may produce a local vasoconstriction in hypoxically aerated lung areas, enabling an intrapulmonary regulation of the V/Q ratio. This is regarded as additional proof that the NEB — while being modulated by the CNS — probably are intrapulmonary chemoreceptors with local secretory activities, reacting to the composition of the inhaled air.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barer, G.R.: The circulation through collapsed adult lungs. J. Physiol. (Lond.) 168, 10 (1963a)

    Google Scholar 

  • Barer, G.R.: The mechanism of the increased vascular resistance caused by hypoxaemia in both collapsed and ventilated lungs. J. Physiol. (Lond.) 169, 102 (1963b)

    Google Scholar 

  • Battaglia, G.: Ultrastructural observations on the biogenic amines in the carotid and aortic-abdominal bodies of the human fetus. Z. Zellforsch. 99, 529–537 (1969)

    Google Scholar 

  • Chapot, G., Barrault, N., Müller, M., Dargnat, N.: Comparative study of PaCO 2in several homoethermic species. Amer. J. Physiol. 223, 1354–1359 (1972)

    Google Scholar 

  • Chiocchio, S.R., Biscardi, A.M., Tramezzani, J.H.: 5-Hydroxytryptamine in the carotid body of the rat. Science 158, 790–791 (1967)

    Google Scholar 

  • Comroe, J.H.: The peripheral chemoreceptors. Handbook of Physiology. Sect. 3, Resp. 1, Chap. 23, pp. 557–583. Baltimore: Waverly Press 1964

    Google Scholar 

  • Comroe, J.H.: Physiology of respiration. Chicago: Year Book Medical Publishers Inc. 1968

    Google Scholar 

  • Daly, I. de B., Hebb, C.: Pulmonary and bronchial vascular systems. Their reactions under controlled conditions of ventilation and circulation. London: E. Arnold Ltd. ed. 1966

    Google Scholar 

  • Daly, I. de B., Wright, P.G.: The effects of anticholinesterases upon pulmonary vascular resistance in the dog. J. Physiol. (Lond.) 139, 273 (1957)

    Google Scholar 

  • Dawes, G.S., Comroe, J.H.: Chemoreflexes from the heart and lungs. Physiol. Rev. 34, 167–201 (1954)

    Google Scholar 

  • Dejours, P.: Chemoreflexes in breathing. Physiol. Rev. 42, 335–358 (1962)

    Google Scholar 

  • Duke, H.N.: The site of action of anoxia on the pulmonary blood vessels of the cat. J. Physiol. (Lond.) 125, 373–382 (1954)

    Google Scholar 

  • Ewen, S.W.B., Bussolati, G., Pearse, A.G.E.: Uptake of L-DOPA and L-5-hydroxytryptophan by endocrine-like cells in the rat larynx. Histochem. J. 4, 103–110 (1972)

    Google Scholar 

  • Erspamer, V.: 5-Hydroxytryptamine and related indolealkylamines. In: Handbuch der experimentellen Pharmakologie, Vol. XIX, p. 245. Berlin-Heidelberg-New York: Springer 1966

    Google Scholar 

  • Falck, B., Owman, C.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic amines. Acta Univ. Lund. 2, 7 (1965)

    Google Scholar 

  • Fishman, A.P.: Respiratory gases in the regulation of the pulmonary circulation. Physiol. Rev. 41, 214–280 (1960)

    Google Scholar 

  • Fishman, A.P.: The pulmonary circulation. In: Effects of anesthetics on the circulation (Price, H.L. and Cohen, P.J. eds.). Springfield, Illinois: Thomas 1964

    Google Scholar 

  • Gaddum, J.H., Hebb, C.O., Silver, A., Swan, A.: 5-Hydroxytryptamine-pharmacological action and destruction in perfused lungs. Quart. J. exp. Physiol. 38, 255–262 (1953)

    Google Scholar 

  • Ginzel, K.H., Kottegoda, S.R.: Study of vascular actions of 5-hydroxytryptamine, adrenaline and noradrenaline. Quart. J. exp. Physiol. 38, 225–231 (1953)

    Google Scholar 

  • Grimelius, L.: A silver nitrate stain for α2-cells in human pancreatic islets. Acta Soc. Med. upsalien. 73, 243–270 (1968)

    Google Scholar 

  • Grimley, P.M., Glenner, G.G.: Ultrastructure of the human carotid body. A perspective on the mode of chemoreception. Circulation 37, 648–665 (1968)

    Google Scholar 

  • Hauge, A.: Hypoxia and pulmonary vascular resistance. The relative effects of pulmonary arterial and alveolar PO2. Acta physiol. scand. 76, 121–130 (1969)

    Google Scholar 

  • Hauge, A., Staub, N.C.: Prevention of hypoxic vasoconstriction in cat lung by histamine-releasing agent 48/80. J. appl. Physiol. 26, 693–699 (1969)

    Google Scholar 

  • Laros, C.D.: Local chemical regulation of the flow resistance in the bronchial tree and pulmonary circulation. Respiration 28, 120–136 (1971)

    Google Scholar 

  • Lauweryns, J.M.: De longvaten: hun architectoniek en hun rol bij de longontplooiing. Thesis, Univ. of Leuven, Arscia, Brussels (1962)

    Google Scholar 

  • Lauweryns, J.M.: L'angioarchitecture du poumon. Arch. Biol. (Liège) 75, 771–811 (1964)

    Google Scholar 

  • Lauweryns, J.M.: The blood and lymphatic microcirculation of the lung. In: Pathology Annual 1971, ed. by S.C. Sommers, pp. 365–415. New York: Appleton-Century-Crofts 1971

    Google Scholar 

  • Lauweryns, J.M., Cokelaere, M.: Intrapulmonary neuro-epithelial bodies: hypoxia sensitive neuro-(chemo) receptors. Experientia (Basel) 29, 1384–1386 (1973a)

    Google Scholar 

  • Lauweryns, J.M., Cokelaere, M.: Hypoxia-sensitive neuro-epithelial bodies: intrapulmonary secretory neuroreceptors, modulated by the CNS. Z. Zellforsch. 145, 521–540 (1973b)

    Google Scholar 

  • Lauweryns, J.M., Cokelaere, M., Deleersnyder, M., Liebens, M.: Intrapulmonary neuro-epithelial bodies in newborn rabbits: influence of hypoxia, hyperoxia, hypercapnia, nicotine, reserpine, L-DOPA and 5-HTP. Cell Tiss. Res. 182, 425–440 (1977)

    Google Scholar 

  • Lauweryns, J.M., Cokelaere, M., Theunynck, P.: Neuro-epithelial bodies in the respiratory mucosa of various mammals: a light optical, histochemical and ultrastructural investigation. Z. Zellforsch. 135, 569–592 (1972)

    Google Scholar 

  • Lauweryns, J.M., Cokelaere, M., Theunynck, P.: Serotonin producing neuro-epithelial bodies in rabbit respiratory mucosa. Science 180, 410–413 (1973)

    Google Scholar 

  • Lauweryns, J.M., Cokelaere, M., Theunynck, P., Deleersnyder, M.: Neuro-epithelial bodies in mammalian respiratory mucosa: light optical, histochemical and ultrastructural studies. Chest 65, 22S-29S (1974)

    Google Scholar 

  • Lauweryns, J.M., Goddeeris, P.: Neuroepithelial bodies in the human child and adult lung. Amer. Rev. resp. Dis. 111, 469–476 (1975)

    Google Scholar 

  • Lauweryns, J.M., Liebens, M.: Microspectrography of formaldehyde and fluorescamine induced fluorescence in rabbit pulmonary neuro-epithelial bodies: demonstration of a new probably polypeptide intracytoplasmic substance. Experientia (Basel) 33, 1510–1511 (1977)

    Google Scholar 

  • Lauweryns, J.M., Peuskens, J.: Argyrophil (kinin and amine producing?) cells in human infant airway epithelium. Life Sci. 8, 577–585 (1969)

    Google Scholar 

  • Lauweryns, J.M., Peuskens, J.: Neuro-epithelial bodies (neuroreceptor or secretory organs ?) in human infant bronchial and bronchiolar epithelium. Anat. Rec. 172, 471–482 (1972)

    Google Scholar 

  • Lauweryns, J.M., Peuskens, J., Cokelaere, M.: Argyrophil, fluorescent and granulated (peptide and amine producing ?) AFG cells in human infant bronchial epithelium; light and electron microscopic studies. Life Sci. 9, 1417–1429 (1970)

    Google Scholar 

  • Lloyd, T.C.: Role of nerve pathway in the hypoxic vasoconstriction of lung. J. appl. Physiol. 21, 1351–1355 (1966)

    Google Scholar 

  • Lloyd, T.C.: Influences of PO2 and pH on resting and active tension of pulmonary arterial strips. J. appl. Physiol. 22, 1101–1109 (1967)

    Google Scholar 

  • Lloyd, T.C.: Hypoxic pulmonary vasoconstriction: role of perivascular tissue. J. appl. Physiol. 25, 560–565 (1968)

    Google Scholar 

  • McLaughin, R.F. Jr., Tyler, W.S., Canada, W.D.: Subgross pulmonary anatomy of the rabbit, rat and guinea pig with additional notes on the human lung. Amer. Rev. resp. Dis. 94, 380–387 (1966)

    Google Scholar 

  • Naeye, R.L.: Pulmonary vascular changes with chronic unilateral pulmonary hypoxia. Circulat. Res. 17, 160–167 (1965)

    Google Scholar 

  • Neutze, J.M., Wyler, F., Rudolph, A.M.: Use of radioactive microspheres to assess distribution of cardiac output in rabbits. Amer. J. Physiol. 215, 486–495 (1968)

    Google Scholar 

  • Nisell, O.: The influence of blood gases on the pulmonary vessels of the cat. Acta physiol. scand. 23, 85–90 (1951)

    Google Scholar 

  • Pearse, A.G.E.: Common cytochemical and ultrastructural characteristics of cells producing polypeptide hormones (the APUD-series) and their relevance to thyroid and ultimo-branchial C-cells and calcitonine. Proc. roy. Soc. B 170, 71–80 (1968)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208 (1963)

    Google Scholar 

  • Rosan, R.C., Lauweryns, J.M.: Secretory cells in the premature human lung lobule. Nature (Lond.) 232, 60–61 (1971)

    Google Scholar 

  • Sjoredsma, A.: Serotonin. New Engl. J. Med. 261, 181–188 (1959)

    Google Scholar 

  • Weibel, E.R., Kistler, G.S., Sherle, W.F.: Practical stereological methods for morphometric cytology. J. Cell Biol. 30, 23–38 (1966)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Katholieke Universiteit te Leuven School of Medicine, Laboratory of Histopathology, Leuven, Belgium

    Joseph M. Lauweryns, Marnix Cokelaere, Toni Lerut & Paul Theunynck

Authors
  1. Joseph M. Lauweryns
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marnix Cokelaere
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toni Lerut
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Paul Theunynck
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lauweryns, J.M., Cokelaere, M., Lerut, T. et al. Cross-circulation studies on the influence of hypoxia and hypoxaemia on neuro-epithelial bodies in young rabbits. Cell Tissue Res. 193, 373–386 (1978). https://doi.org/10.1007/BF00225336

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00225336

Key words

Search

Navigation