Abstract
RFLP marker data from an F2∶3 population derived from a cross between a sugary1 (su1) and a sugary enhancer1 (su1, sel) inbred were used to construct a genetic linkage map of maize. This map includes 93 segregating marker loci distributed throughout the maize genome, providing a saturated linkage map that is suitable for linkage analysis with quantitative trait loci (QTL). This population, which has been immortalized in the form of sibbed F2∶3 families, was derived from each of the 214 F2 plants and along with probe data are available to the scientific community. QTL analysis for kernel sucrose (the primary form of sugar) concentration at 20 days after pollination (DAP) uncovered the segregation of seven major QTL influencing sucrose concentration; a locus linked to umc36a described the greatest proportion of the variation (24.7%). Since maltose concentration has previously been reported to be associated with the se1 phenotype, an analysis of probe associations with maltose concentration at 40 DAP was also conducted. The highly significant association of umc36a with maltose and sucrose concentrations provided evidence that this probe is linked to se1. Phenotypic evaluation for the se1 genotype in each F2∶3 family enabled us to map the gene 12.1 cM distal to umc36a. In contrast to previous work where se1 was reported to be located on chromosome four, our data strongly suggest that the sugary enhancer1 locus maps on the the distal portion of the long arm of chromosome 2 in the maize genome.
Similar content being viewed by others
References
Anderson E, Brown WL (1952) Origin of Corn Belt maize and its genetic significance. In: Gowen JW (ed) Heterosis. Iowa State College Press, Ames, Iowa, pp 124–148
Azanza F, Young TE, Kim D, Tanksley SD, Juvik JA (1994) Characterization of the effect of introgressed segments of chromosome 7 and 10 from Lycopersicon chmielewskii on tomato soluble solids, pH and yield. Theor Appl Genet 87:965–972
Beavis WD, Grant D (1991) A linkage map based on information from four F2 populations of maize. Theor Appl Genet 82:636–644
Beckett JB (1978) B-A translocations in maize. J Hered 69:27–36
Bhave MR, Lawrence S, Barton C, Hannah LC (1990) Identification and molecular characterization of shrunken-2 cDNA clones of maize. Plant Cell 2:581–588
Boyer CD, Shannon JC (1983) The use of endosperm genes for sweet corn improvement. Plant Breed Rev 1:139–161
Burr B, Burr FA (1991) Recombinant inbreds for molecular mapping in maize; theoretical and practical considerations. Trends Genet 7:55–60
Burr B, Burr FA, Matz EC, Romero-Severson J (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:953–960
Carey EE, Rhodes AM, Dickinson DB (1982) Post-harvest levels of sugars and sorbitol in sugary enhancer (su1se1) and sugary (su1Se1) maize. HortScience 17:241–242
Close TJ, Kortt AA, Chander PM (1989) A cDNA-based comparison of dehydrin-induced protein (dehydrin) in barley and corn. Plant Mol Biol 13:95–108
Coe EH (1993) Gene list and working maps. Maize Genet Coop Newsl 67:133–166
Coe EH, Neuffer MG, Hoistington DA (1988) The genetics of corn. In: Sprague GF, Dudley JW (eds) Corn and corn improvement. American Society of Agronomy, Madison Wis., pp 81–258
Darvasi A, Weinreb A, Minke V, Weller JI, Soller M (1993) Detecting marker-QTL linkage and estimating QTL gene effect and map location using a saturated genetic map. Genetics 134:943–951
Dickinson DB, Boyer CD, Velu JG (1983) Reserve carbohydrates from kernels of sugary and sugary enhancer maize. Phytochemistry 22:1371–1373
Dickinson DB, Juvik JA, Shaw JR (1991) Identification of maize inbreds with high levels of maltose in endosperm of germinating seeds. HortScience 25:1660
Edwards MD, Stuber CW, Wendel JF (1987) Molecular-marker facilitated investigations of quantitative trait loci in maize. I. Numbers, genomic distribution and types of gene action. Genetics 116:113–125
Feinberg A, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Ferguson JE, Rhodes AM, Dickinson DB (1978) The genetics of sugary enhancer (se), an independent modifier of sweet corn (su). J Hered 69:377–380
Ferguson JE, Dickinson DB, Rhodes AM (1979) Analysis of endo-sperm sugars in a sweet corn inbred (Illinois 677a) which contains the sugary enhancer (se1) gene and comparison of se1 with other corn genotypes. Plant Physiol 63:377–380
Gardiner JM, Coe EH, Melia-Hancock S, Hoistington DA, Chao S (1993) Development of a core RFLP map in maize using an immortalized F2 population. Genetics: 134:917–930
Gonzales JW, Rhodes AM, Dickinson DB (1974) A new inbred with high sugar content in sweet corn. HortScience 9:79–80
Gonzales JW, Rhodes AM, Dickinson DB (1976) Carbohydrate and enzymatic characterization of a high sucrose inbred in sweet con. Plant Physiol 58:28–32
Helentjaris TM, Slocum M, Wright S, Schaffer A, Nienhuis J (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphism. Theor Appl Genet 72:761–769
Hoistington DA, Coe EH, Neuffer MG (1988) Gene list and linkage map of maize (Zea mays L.). Maize Genet Coop Newsl 62:125–147
Juvik JA, La Bonte DR (1988) Single-kernel analysis for the presence of sugary enhancer (se) gene in sweet corn. HortScience 23:384–386
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
La Bonte DR, Juvik JA (1990) Characterization of sugary1 (su1) and sugary enhancer (se) kernels in segregating maize (Zea mays L.) populations. J Am Soc Hortic Sci 114:153–157
La Bonte DR, Juvik JA (1991) Sugary enhancer (se) gene is located on the long arm of chromosome 4 in maize (Zea mays L). J Hered 82:176–178
Lander ES, Green P, Abrahmson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Marshall SW (1987) Sweet corn. In: Watson SA, Ramstad PE (eds) Corn: chemistry and technology. Am Assoc Cereal Chemist, St Paul, Minn., pp 431–445
Pan D, Nelson OE (1984) A debranching enzyme deficiency in the endosperm of sugary-1 mutants of maize. Plant Physiol 74:324–328
Saghai-Maroof MA, Soliman KL, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
SAS Institute Inc (1988) SAS users guide: statistics, 6th edn. SAS Institute, Cary, N. C.
Shaw JR, Hannah LC (1992) Genomic nucleotide sequence of wild type shrunken-2 alleles of Zea mays. Plant Physiol 98:1214–1216
Sheldon E, Ferl R, Federoff N, Hannah LC (1983) Isolation and analysis of a genomic clone encoding sucrose synthase in maize: evidence for two introns in sh1. Mol Gen Genet 190:421–426
Tanksley SD (1993) Mapping polygenes. Annu Rev Genet 27:205–233
Tapley WT, Enzie WD, Van Eseltine GP (1934) Sweet corn. In: The vegetables of New York. N Y State Agric Exp Stn Rep, p 233
Author information
Authors and Affiliations
Additional information
Communicated by M. Koorneef
Rights and permissions
About this article
Cite this article
Tadmor, Y., Azanza, F., Han, T. et al. RFLP mapping of the sugary enhancer1 gene in maize. Theoret. Appl. Genetics 91, 489–494 (1995). https://doi.org/10.1007/BF00222978
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222978