Abstract
The human interleukin-6 receptor α chain (IL6Rα) gene was regionally mapped to chromosome 1 bridization probes, partially overlapping lambda clones encompassing 28 kb of the genomic region of the gene were used. These clones were isolated using a polymerase chain reaction (PCR)-generated fragment of the 3′ noncoding region of the gene. This localization confirms and extends the provisional assignment of the IL6Rα gene to chromosome 1, for which a panel of somatic cell hybrids was used.
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Bazan JF (1990) Structural design and molecular evolution of a cytokine receptor superfamily. Proc Natl Acad Sci USA 87:6934–6938
Kaashoek JGJ, Mout R, Falkenburg JHF, Willemze R, Fibbe WE, Landegent JE (1991) Cytokine production by the bladder carcinoma cell line 5673:Rapid analysis of mRNA expression levels using a cDNA-PCR procedure. Lymphokine Cytokine Res 10:231–235
Kawano M, Hirano T, Matsuda T, Taga T, Horii Y, Iwato K, Asaoku H, Tang B, Tanabe O, Tanaka H, Kuramoto A, Kishimoto T (1988) Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature 332:83–85
Kishimoto T (1989) The biology of interleukin-6. Blood 74:1–10
Landegent JE, Jansen in de Wal N, Dirks RW, Baas F, Ploeg van der M (1987) Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization. Hum Genet 77:366–370
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Sugita T, Totsuka T, Saito M, Yamasaki K, Taga T, Hirano T, Kishimoto T (1990) Functional murine interleukin 6 receptor with the intracisternal A particle gene product at its cytoplasmic domain. J Exp Med 171:2001–2009
Sutherland GR, Baker E, Callen DF, Hyland VJ, Wong G, Clark S, Jones SS, Eglinton LK, Shannon MF, Lopez AF, Vadas MA (1988) Interleukin 4 is at 5q31 and interleukin 6 is at 7p15. Hum Genet 79:335–337
Szpirer J, Szpirer C, Riviere M, Houart C, Baumann M, Fey GH, Poli V, Cortese R, Islam MQ, Levan G (1991) The interleukin-6-dependent DNA-binding protein gene (transcription factor 5:TCF5) maps to human chromosome 20 and rat chromosome 3, the IL6 receptor locus (IL6R) to human chromosome 1 and rat chromosome 2, and the rat IL6 gene to rat chromosome 4. Genomics 10:539–546
Taga T, Hibi M, Hirata Y, Yamasaki K, Yasukawa K, Matsuda T, Hirano T, Kishimoto T (1989) Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130. Cell 58:573–581
Trent JM, Kaneko Y, Mitelman F (1989) Report of the committee on structural chromosome changes in neoplasia. Cytogenet Cell Genet 51:533–562
Vink A, Coulie P, Warnier G, Renauld JC, Stevens M, Donckers D, van Snick J (1990) Mouse plasmacytoma growth in vivo: Enhancement by interleukin 6 (IL-6) and inhibition by antibodies directed against IL-6 or its receptor. J Exp Med 172:997–1000
Wiegant J, Galjart NJ, Raap AK, d'Azzo A (1991) The gene encoding human protective protein (PPGB) is on chromosome 20. Genomics 10:345–349
Yamasaki K, Taga T, Hirata Y, Yawata H, Kawanishi Y, Seed B, Taniguchi T, Hirano T, Kishimoto T (1988) Cloning and expression of the human interleukin-6 (BSF-2/INFβ 2) receptor. Science 241:825–828
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Kluck, P.M.C., Wiegant, J., Jansen, R.P.M. et al. The human interleukin-6 receptor α chain gene is localized on chromosome 1 band q21. Hum Genet 90, 542–544 (1993). https://doi.org/10.1007/BF00217455
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DOI: https://doi.org/10.1007/BF00217455