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Regulation of sedoheptulose-1,7-bisphosphatase by sedoheptulose-7-phosphate and glycerate, and of fructose-1,6-bisphosphatase by glycerate in spinach chloroplasts

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Abstract

Using partially purified sedoheptulose-1,7-bisphosphatase from spinach (Spinacia oleracea L.) chloroplasts the effects of metabolites on the dithiothreitoland Mg2+-activated enzyme were investigated. A screening of most of the intermediates of the Calvin cycle and the photorespiratory pathway showed that physiological concentrations of sedoheptulose-7-phosphate and glycerate specifically inhibited the enzyme by decreasing its maximal velocity. An inhibition by ribulose-1,5-bisphosphate was also found. The inhibitory effect of sedoheptulose-7-phosphate on the enzyme is discussed in terms of allowing a control of sedoheptulose-1,7-bisphosphate hydrolysis by the demand of the product of this reaction. Subsequent studies with partially purified fructose-1,6-bisphosphatase from spinach chloroplasts showed that glycerate also inhibited this enzyme. With isolated chloroplasts, glycerate was found to inhibit CO2 fixation by blocking the stromal fructose-1,6-bisphosphatase. It is therefore possible that the inhibition of the two phosphatases by glycerate is an important regulatory factor for adjusting the activity of the Calvin cycle to the ATP supply by the light reaction.

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Abbreviations

DTT:

dithiothreitol

FBPase:

fructose-1,6-bisphosphatase

Fru-1,6-P2 :

fructose-1,6-bisphosphate

Fru-6-P:

fructose-6-phosphate

3-PGA:

3-phosphoglycerate

Ru-1,5-P2 :

ribulose-1,5-bisphosphate

Ru-5-P:

ribulose-5-phosphate

SBPase:

sedoheptulose-1,7-bisphosphatase

Sed-1,7-P2 :

sedoheptulose-1,7-bisphosphate

Sed-7-P:

sedoheptulose-7-phosphate

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Authors and Affiliations

  1. Institut für Biochemie der Pflanze, Universität Göttingen, Untere Karspüle 2, D-3400, Göttingen, Germany

    Dieter Schimkat, Dieter Heineke & Hans W. Heldt

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  1. Dieter Schimkat
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  2. Dieter Heineke
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  3. Hans W. Heldt
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This work was supported by the Deutsche Forschungsgemein-schaft.

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Schimkat, D., Heineke, D. & Heldt, H.W. Regulation of sedoheptulose-1,7-bisphosphatase by sedoheptulose-7-phosphate and glycerate, and of fructose-1,6-bisphosphatase by glycerate in spinach chloroplasts. Planta 181, 97–103 (1990). https://doi.org/10.1007/BF00202330

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  • DOI: https://doi.org/10.1007/BF00202330

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