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A carcinoembryonic antigen (CEA) specific monoclonal hybridoma antibody that reacts only with high-molecular-weight CEA

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Summary

A monoclonal antibody secreted by a hybridoma, formed by the fusion of a spleen cell from an immunized mouse and a myeloma cell, has been shown to react only with a form of carcinoembryonic antigen (CEA) with a molecular weight of 180,000 daltons. The monoclonal antibody precipitates a 180,000-dalton form of CEA from colorectal carcinoma cell lysates, purified iodinated CEA and iodinated CEA-S, but does not precipitate the ‘cross-reactive’ antigen (NCA) from normal colon.

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References

  1. Coligan JE, Lautenschleger JT, Egan ML, Todd CW (1972) Isolation and characterization of carcinoembryonic antigen. Immunochemistry 99:377

    Google Scholar 

  2. Dent PB, Carrel S, Mach J-P (1980) Detection of new cross-reacting antigen(s) on cultured tumor cells by mixed hemadsorption assay. J Natl Cancer Inst 64:309

    Google Scholar 

  3. Edgington TS (1975) CEA-S: A distinctive isomeric variant of carcinoembryonic antigen. Fed Proc 34:845

    Google Scholar 

  4. Fritsche R, Mach J-P (1977) Isolation and characterization of carcinoembryonic antigen (CEA) extracted from normal human colon mucosa. Immunochemistry 14:119

    Google Scholar 

  5. Giovanella BD, Stehlin JS, Santamaria C, Yim SO, Morgan AC, Williams LJ, Jr, Leibovitz A, Fialkow PJ, Mumford DM (1976) Human neoplastic and normal cells in tissue culture. I. Cell lines derived from malignant melanomas and normal melanocytes. J Natl Cancer Inst 56:1131

    Google Scholar 

  6. Gold P, Freedman SO (1965a) Demonstration of tumor-specific antigens in human carcinomata by immunological tolerance and absorption techniques. J Exp Med 121:439

    Google Scholar 

  7. Gold P, Freedman SO (1965b) Specific carcinoembryonic antigen of the human digestive system. J Exp Med 122:467

    Google Scholar 

  8. Hansen HJ (1974) Carcinoembryonic antigen (CEA) assay. A laboratory adjunct of the diagnosis and management of cancer. Hum Pathol 5:139

    Google Scholar 

  9. Hedin A, Hammarstrom S, Svenberg T, Swinblad G (1978) Low molecular weight CEA, isolation and characterization. Scand J Immunol [Suppl] 8:423

    Google Scholar 

  10. Kearney JF, Radbruch A, Liesegang B (1979) A new mouse myeloma cell line which has lost immunoglobulin expression but permits the construction of antibody secreting hybridomas. J Immunol 123:1548

    Google Scholar 

  11. Koprowski H, Steplewski Z, Herlyn D, Herlyn M (1978) Study of antibodies against human melanoma produced by somatic cell hybrids. Proc Natl Acad Sci USA 75:3405

    Google Scholar 

  12. Koprowski H, Steplewski Z, Mitchell K, Herlyn M, Herlyn D, Fuhrer P (1979) Colorectal carcinoma antigens detected by hybridoma antibodies. Som Cell Genet 5:957

    Google Scholar 

  13. Laemmli UK (1970) Cleavage of structural proteins somatic cell genetics during assembly of the head of bacteriophage T4. Nature 227:680

    PubMed  Google Scholar 

  14. Leung JP, Eshdat Y, Marchesi VT (1977) Colonic tumor membrane-associated glycoprotein: Isolation of antigenically-active peptides after chemical cleavage. J Immunol 119:664

    Google Scholar 

  15. Lo Gerfo P, Krupey J, Hansen HJ (1971) Demonstration of an antigen common to several varieties of neoplasia. N Engl J Med 285:138

    Google Scholar 

  16. Marchalonis JJ, Cone RE, Santer V (1971) A probe for accessible surface proteins of normal and neoplastic lymphocytes. Biochem J 124:921

    Google Scholar 

  17. Moore TL, Kupchik HZ, Marcon N, Zamcheck N (1971) Carcinoembryonic antigen assay in cancer of the colon and pancreas and other digestive tract disorders. Am J Dig Dis 16:1

    Google Scholar 

  18. Morgan G, McCarthy WH, Hersey P (1977) Detection of carcinoembryonic-like antigen on melanoma cells by leucocyte-dependent-antibody assays. Br J Cancer 36:446

    Google Scholar 

  19. Plow EF, Edgington TS (1975) Isolation and characterization of a homogeneous isomeric species of carcinoembryonic antigen: CEA-S. Int J Cancer 15:748

    Google Scholar 

  20. Posztaszeri G, Mach J-P (1973) Carcinoembryonic antigen (CEA) in non-digestive cancerous and normal tissues. Immunochemistry 10:197

    Google Scholar 

  21. Thomson DMP, Krupey J, Freedman SO (1969) The radio-immunoassay of circulating carcinoembryonic antigens of the human digestive system. Proc Natl Acad Sci USA 64:161

    Google Scholar 

  22. Tomita JT, Safford JW, Hirata AA (1974) Antibody response to different determinants on carcinoembryonic antigen (CEA). Immunology 26:291

    Google Scholar 

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  1. The Wistar Institute of Anatomy and Biology, 36th Street at Spruce, 19104, Philadelphia, Pennsylvania, USA

    Kenneth F. Mitchell

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  1. Kenneth F. Mitchell
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Mitchell, K.F. A carcinoembryonic antigen (CEA) specific monoclonal hybridoma antibody that reacts only with high-molecular-weight CEA. Cancer Immunol Immunother 10, 1–5 (1980). https://doi.org/10.1007/BF00199272

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  • DOI: https://doi.org/10.1007/BF00199272

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