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Formation of heartwood substances in the stemwood of Robinia pseudoacacia L. II. Distribution of nonstructural carbohydrates and wood extractives across the trunk

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The distributions of reserve carbohydrates and of three dominant heartwood extractives were determined in the trunkwood of Robinia pseudoacacia L. The trees were cut at different times of the year (September, November, January, and April). With the exception of the tree felled in January, all trunks exhibited highest contents of nonstructural storage carbohydrates (glucose, fructose, sucrose, and starch) in the youngest, outermost sapwood zone. With increasing depth of the trunk, the levels of carbohydrates decreased. At the sapwood-heartwood transition zone, only trace amounts of nonstructural carbohydrates were present. The heartwood itself contained no storage material. The wood zones of different ages of the trees cut in September, November, and January exhibited glucose/fructose ratios of approximately 1. In April, however, there was a shift to glucose. In the youngest sapwood the amounts of soluble sugars were higher in the earlythan in the latewood. Older zones of the sapwood and the sap-wood-heartwood transition zone showed the opposite behaviour. Three main wood extractives of Robinia were characterized and quantified: the flavanonol dihydrorobinetin (DHR), the flavonol robinetin (ROB) and a hydroxycinnamic acid derivative (HCA). Only DHR was present — in very low amounts — in the younger sapwood of all trunks investigated. Higher amounts (>1 μmol/g dry weight) of this compound and the HCA were present in the sapwood-heartwood transition zone. DHR augmented within the heartwood up to a more or less constant level. HCA increased towards the heartwood and decreased again in the inner heartwood parts. ROB appeared in the innermost parts of the sapwood-heartwood transition zone and reached maximum values in older parts of the heart-wood. The results indicate that starch is hydrolyzed at the sapwood-heartwood boundary and thus represents a primary major source of hydroxycinnamic acid and flavonoid synthesis.

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References

  • Auger MA, Jay-Allemand C, Bastien C, Geri C (1991) Comestibilité de differents clones de pin sylvestre pour Diprion pini L. (Hym., Diprionidae). II. Relations entre le contenu phénolique des aiguilles de pins sylvestres et la mortalité des larves de Diprion pini. J Appl Ent 111: 78–85

    Google Scholar 

  • Baqui SA, Shah JJ (1985) Histoenzymatic studies in wood of Acacia auriculiformis Cunn. during heartwood formation. Holzforschung 39: 311–320

    Google Scholar 

  • Carll C, Eslyn W, Myers G, Brewer W, Staton D (1985) Evaluation of black locust (Robinia pseudoacacia) as raw material for wet-process hardboard. For Prod J 35: 11–17

    Google Scholar 

  • Datta SK, Kumar A (1987) Histochemical studies of the transition from sapwood to heartwood in Tectona grandis. IAWA Bull n. s. 8: 363–368

    Google Scholar 

  • Dietrichs HH (1964) Das Verhalten von Kohlenhydraten bei der Holzverkernung. Holzforschung 18:14–24

    Google Scholar 

  • Fischer C, Höll W (1992) Food reserves of scots pine (Pinus sylvestris). II. Seasonal changes and radial distribution of carbohydrates and fat reserves in pine wood. Trees 6: 147–155

    Google Scholar 

  • Freudenberg K, Hartmann L (1953) Constituents from Robinia pseudoacacia. Naturwiss 40: 413

    Google Scholar 

  • Hahlbrock K (1981) Flavonoids. In: EE Conn (ed) The Biochemistry of plants, vol 7. Secondary plant products, Academic Press, New York, pp 425–456

    Google Scholar 

  • Harborne JB (1989) Flavonoids. In: Rowe JW (ed) Natural products of woody plants. Springer, Berlin Heidelberg New York, pp 533–570

    Google Scholar 

  • Hart JH (1989) The role of wood exudates and extractives in protecting wood from decay. In: Rowe JW (ed) Natural products of woody plants. Springer, Berlin Heidelberg New York, pp 861–880

    Google Scholar 

  • Higuchi T, Onda Y, Fujimoto Y (1969) Biochemical aspects of heartwood formation with special reference to the site of biogenesis of heartwood compounds. Wood Res 48: 1530

    Google Scholar 

  • Hillis WE (1958) Formation of condensed tannins in plants. Nature 182: 1371

    Google Scholar 

  • Hillis WE (1987) Heartwood and tree exudates. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Hillis WE (1989) Historical use of extractives and exudates In: Rowe JW (ed) Natural products of woody plants. Springer, Berlin Heidelberg New York, pp 1–13

    Google Scholar 

  • Hillis WE, Hasegawa M (1963) The formation of polyphenols in trees. I. Administration of 14C-glucose and subsequent distribution of radioactivity. Phytochem 2: 195–199

    Google Scholar 

  • Hillis WE, Humphreys FR, Bamber RK, Carle A (1962) Factors influencing the formation of phloem and heartwood polyphenols. II. The availability of stored and translocated carbohydrates. Holzforschung 16: 114–121

    Google Scholar 

  • Höll W (1972) Stärke und Stärkeenzyme im Holz von Robinia pseudoacacia L. Holzforschung 26: 41–45

    Google Scholar 

  • Höll W, Lendzian K (1973) Respiration in the sapwood and heartwood of Robinia pseudoacacia. Can J Bot 52: 727–734

    Google Scholar 

  • Imamura H (1989) Contribution of extractives to wood characteristics In: Rowe JW (ed) Natural products of woody plants. Springer, Berlin Heidelberg New York, pp 843–860

    Google Scholar 

  • Kumar A, Datta SK (1989) Histochemical and histoenzymological changes during heartwood formation in a timber tree Shorea robusta. Proc Indian Sci (Plant Sci) 99: 21–27

    Google Scholar 

  • Magel EA, Höll W (1993) Storage carbohydrates and adenine nucleotides in trunks of Fagus sylvatica L. in relation to discolored wood. Holzforschung 47: 19–24

    Google Scholar 

  • Magel EA, Drouet A, Claudot AC, Ziegler H (1991) Formation of heartwood substances in the stemwood of Robinia pseudoacacia L. I. Distribution of phenylalanine ammonium-lyase and chalcone synthase across the trunk. Trees 5: 203–207

    Google Scholar 

  • Nair MNB (1988) Wood anatomy and heartwood formation in Neem (Azadirachta indica A. Juss.). Bot J Linn Soc 97: 79–90

    Google Scholar 

  • Nobuchi T, Harada H (1983) Physiological features of the “white zone” of Sugi (Cryptomeria japonica D. Don). Cytological structure and moisture content. Mokuzai Gakkaishi 29: 824–832

    Google Scholar 

  • Nobuchi T, Kuroda K, Iwata R, Harada H (1982) Cytological study of the seasonal features of heartwood formation of Sugi (Cryptomeria japonicav D. Don). Mokuzai Gakkaishi 28: 669–676

    Google Scholar 

  • Nobuchi T, Sato T, Iwata R, Harada H (1984) Season of heartwood formation and the related cytological structure of ray parenchyma cells in Robinia pseudoacacia L. Mokuzai Gakkaishi 30: 628–636

    Google Scholar 

  • Nobuchi T, Takai K, Harada H (1987a) Distribution of heartwood phenols in the trunk of Sugi (Cryptomeria japonica D. Don) and partial characterisation of heartwood formation. Mokuzai Gakkaishi 33: 88–96

    Google Scholar 

  • Nobuchi T, Tokuchi N, Harada H (1987b) Variability of heartwood formation and cytological features in broadleaved trees. Mokuzai Gakkaishi 33: 596–604

    Google Scholar 

  • Nyárs J (1985) Experiments in particleboard manufacture. Faipar 35: 16–18

    Google Scholar 

  • Pine A, Mullins MG (1976) Changes in anthocyanin and phenolic content of grapevine leaf and fruit tissues treated with sucrose, nitrate, and abscisic acid. Plant Physiol 58: 468–472

    Google Scholar 

  • Pomeroy MK, Siminovitch D (1971) Seasonal cytological changes in secondary phloem parenchyma cells in Robinia pseudoacacia in relation to cold hardiness. Can J Bot 49: 787–795

    Google Scholar 

  • Putman LJ, Laks PE, Pruner MS (1989) Chemical constituents of black locust bark and their biocidal activity. Holzforschung 43: 219–224

    Google Scholar 

  • Ravanel P, Creuzet S, Tissut M (1990) Inhibitory effect of hydroxyflavones on the exogenous NADH dehydrogenase of plant mitochondrial inner membranes. Phytochem 29: 441–445

    Google Scholar 

  • Roux DG, Paulus E (1962) Condensed tannins. 13. Interrelationships of flavonoid components from the heartwood of Robinia pseudoacacia. Biochem J 82: 324–330

    Google Scholar 

  • Saranpää P, Höll W (1989) Soluble carbohydrates of Pinus sylvestris L. sapwood and heartwood. Trees 3: 138–143

    Google Scholar 

  • Sauter JJ, Marquardt H (1966) Untersuchungen zur Physiologie der Pappelholzstrahlen. I. Jahresperiodischer Verlauf der Stärkespeicherung im Holzstrahlparenchym. Z Pflanzenphysiol 55: 246–258

    Google Scholar 

  • Shah JJ, Baqui S, Pandalai RC, Patel KR (1981) Histochemical changes in Acacia nilotica L. during transition from sapwood to heartwood. IAWA Bull n. s. 2: 31–36

    Google Scholar 

  • Shain L (1977) The effect of extractives from black locust heartwood on Fomus rimosus and other decay fungi. Proc Am Phytopath Soc 3: 216 (Abstract)

    Google Scholar 

  • Smith AL, Campbell CL, Walker DB, Hanover JW (1989 a) Extracts from black locust as wood preservatives: extraction of decay resistance from black locust heartwood. Holzforschung 43: 293–296

    Google Scholar 

  • Smith AL, Campbell CL, Diwakar MP, Hanover JW, Miller RO (1989 b) Extracts from black locust as wood preservatives: A comparison of the methanol extract with pentachlorphenol and chromated copper arsenate. Holzforschung 43:421–423

    Google Scholar 

  • Stringer JW, Carpenter SB (1986) Energy yield of black locust biomass fuel. For Sci 32: 1049–1057

    Google Scholar 

  • Treutter G, Galensa R, Feucht W, Schmid PPS (1985) Flavanone glucoside in callus and phloem of Prunus avium: identification and stimulation of their synthesis. Physiol Plant 65: 95–101

    Google Scholar 

  • Ziegler H (1968) Biologische Aspekte der Kernholzbildung. Holz Roh-Werkst 26:61–68

    Google Scholar 

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Dedicated to Prof. Meinhart H. Zenk on the occasion of his 60th birthday

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Magel, E., Jay-Allemand, C. & Ziegler, H. Formation of heartwood substances in the stemwood of Robinia pseudoacacia L. II. Distribution of nonstructural carbohydrates and wood extractives across the trunk. Trees 8, 165–171 (1994). https://doi.org/10.1007/BF00196843

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