Abstract
Platelet aggregation and interaction with other vascular cells play a key role in hemostatic events during hemodialysis. We studied seven patients with end-stage renal failure on long-term hemodialysis treatment. Flow-cytometric techniques and platelet-specific monoclonal antibodies were used to measure the platelet surface expression of glycoproteins - fibrinogen receptor on glycoprotein IIb-IIIa (GP IIb-IIIa; CD41) and α-granule membrane protein (GMP-140; CD62). In addition, adhesion of platelets or platelet microparticles with leukocytes was evaluated by appearance of the platelet-specific antigen (GP IIb-IIIa) on leukocytes. Blood samples were taken before the start of dialysis and 15, 60, and 240 min thereafter. There was a significant increase in fibrinogen receptor activation on circulating platelets after 15 min of dialysis treatment (P < 0.001) and enhanced degranulation of GMP-140 (P < 0.05). In parallel, the interaction of platelets with neutrophils and monocytes also increased with the duration of dialysis and was maximal after 15 min (P < 0.001). We conclude that the platelet fibrinogen receptor on GP IIb-IIIa in circulating platelets is activated during hemodialysis and is associated with increased adhesion of platelets or platelet microparticles with circulating leukocytes. Thus, the phenomenon described here of platelet-leukocyte interaction could be pathophysiologically important for the development of dialysis-associated leukopenia.
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Abbreviations
- GP IIb-IIIa:
-
glycoprotein IIb-IIIa
- GMP140:
-
granule membrane protein 140
- LIBS:
-
ligand-induced binding site
- FACS:
-
flow-activated cell sorting
- mAb:
-
monoclonal antibody
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Gawaz, M., Bogner, C. Changes in platelet membrane glycoproteins and platelet-leukocyte interaction during hemodialysis. Clin Investig 72, 424–429 (1994). https://doi.org/10.1007/BF00180515
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DOI: https://doi.org/10.1007/BF00180515