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Exopolysaccharide production and attachment strength of bacteria and diatoms on substrates with different surface tensions

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Abstract

Attachment strength and exopolysaccharide (EPS) production of Pseudomonas sp. (bacteria) and the diatom Amphora coffaeformis were studied on six different substrata with surface tensions between 19 and 64.5 mN m−1. Test panels of the materials were exposed to bacterial cultures between 3 and 120 hours, and to diatom cultures between 48 and 72 hours. Exopolysaccharide production by surface-associated cells was measured using the phenol sulfuric acid method. Attachment studies were run by exposing test panels to laminar flow pressure using a radial flow chamber. Highest EPS production by bacteria and diatoms was recorded on substrata with surface tensions above 30 mN m−1. Lowest EPS production occurred on substrata between 20 and 25 mN m−1. Highest EPS production and strongest adhesion was found on polycarbonate (33.5 mN m−1). Both test organisms improved their attachment strength with exposure time on most materials. However, amounts of produced EPS and improvement of attachment indicated that mechanisms other than polysaccharide production are more important on substrata with low surface tensions (<25 mN m−1). Simply producing more polysaccharides is not sufficient to overcome weak attachment on materials with low surface tensions. For example, adhesion of Pseudomonas sp. and A. coffaeformis on polytetrafluorethylene/perfluor-copolymer (PFA; 22 mN m−1). and glass (64.5 mN m−1. was equally strong although EPS production was much higher on glass than on PFA. This is somewhat surprising for A. coffaeformis because polysaccharide production has been considered the most important attachment mechanism of A. coffaeformis.

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References

  1. Absolom DR, Lamberti FV Policova Z, Zingg W. van Oss CJ, Neumann AW (1983) Surface thermodynamics of bacterial adhesion. Appl Environ Microbiol 46:90–97

    Google Scholar 

  2. Abu GO, Weiner RM, Rice J, Colwell RR (1991) Properties of an extracellular adhesive polymer from the marine bacterium Schewanella colwelliana. Biofouling 3:69–84

    Google Scholar 

  3. Allison DG, Sutherland IW (1984) A staining technique for attached bacteria and its correlation to extracellular carbohydrate production. J Microbiol Meth 2:93–99

    Google Scholar 

  4. Allison DG, Sutherland IW (1987) The role of exopolysaccharides in adhesion of freshwater bacteria. J Gen Microbiol 133:1319–1327

    Google Scholar 

  5. Baier RE (1973) Influence of the initial surface condition of materials on bioadhesion. In: Acker RE, Brown BF, DePalma JR, Iverson WP (eds) Proc 3rd Int Congress on Marine Corrosion and Fouling, Nat Bur Standards, Gaithersburg, pp 633–639

    Google Scholar 

  6. Baier RE, Shafrin EG, Zisman WA (1968) Adhesion: mechanisms that assist or impede it. Science 162:1360–1368

    Google Scholar 

  7. Becker K, Wahl M (1991) Influence of substratum surface tension on biofouling of artificial substrata in Kiel Bay Western Baltic): in situ studies. Biofouling 4:275–291

    Google Scholar 

  8. Bendinger B, Rijnhaarts HH, Altendorf K-H, Zehnder JB (1993) Physicochemical cell surface and adhesive properties of coryneform bacteria related to the presence and chain length of mycolic acids. Appl Environ Microbiol 59:3973–3977

    Google Scholar 

  9. Busscher HJ, Weerkamp AH (1987) Specific and nonspecific interactions in bacterial adhesion to solid substrata. FEMS Microbiol Rev 46:165–173

    Google Scholar 

  10. Chamberlain AHL (1976) Algal settlement and secretion of adhesive materials. In: Sharpley JM, Kaplan AM (eds) Proc 3rd Int Biodegrad Symposium, Applied Science, London, pp 417–432

    Google Scholar 

  11. Characklis WG, Cooksey KE (1983) Biofilms and microbial fouling. Adv Appl Microbiol 29:93–138

    Google Scholar 

  12. Christensen BE, Kjosbakken J, Smidsrod O (1985) Partial chemical and physical characterization of two extracellular polysaccharides produced by marine periphytic Pseudomonas sp. strain NCMB 2021. Appl Environ Microbiol 50(4):837–845

    Google Scholar 

  13. Cooksey KE, Cooksey B (1986) Adhesion of fouling diatoms to surfaces: some biochemistry. In: Evans LV, Hoagland KD (eds) Algal biofouling. Elsevier, Amsterdam, pp 41–53

    Google Scholar 

  14. Cooksey B, Cooksey KE, Miller CA, Paul JH, Webster D (1984) The attachment of microfouling diatoms. In: Costlow JD, Tipper RC (eds) Marine corrosion and biodeterioration—an interdisciplinary study. E & FN Spon Ltd, London, pp 167–171

    Google Scholar 

  15. Corpe WA (1970) An acid polysaccharide produced by a primary film-forming marine bacterium. Dev Ind Microb 11:402–412

    Google Scholar 

  16. Corpe WA (1980) Microbial surface components involved in adsorption onto surfaces. In: Bitton G, Marshall KC (eds) Adsorption of microorganisms to surfaces. John Wiley & Sons, Wiley Intersci Publ, New York, pp 105–143

    Google Scholar 

  17. Costerton JW, Cheng K-J, Geesey GG, Ladd TI, Nickel JC, Dasgupta M, Marrie TJ (1987) Bacterial biofilms in nature and disease. Ann Rev Microbiol 41:435–464

    Google Scholar 

  18. Daniel GF, Chamberlain AHL, Jones EBG (1980) Ultrastructural observations on the marine fouling algae Amphora. Helgol Wiss Meeresunters 34:123–149

    Google Scholar 

  19. Decho AW (1990) Microbial exopolymer secretions in ocean environments: their role(s) in food webs and marine processes. Oceanogr Mar Ann Rev 28:73–153

    Google Scholar 

  20. Devasia P, Natarajan KA, Sathyanarayana DN, Rao GR (1993) Surface chemistry of Thiobacillus ferrooxidans relevant to adhesion on mineral surfaces. Appl Environ Microbiol 59:4051–4055

    Google Scholar 

  21. Dexter SC (1976) Influence of substrate wettability on the formation of bacterial slime films on solid surfaces immersed in natural seawater. In: Romanovsky V (ed) Proc 4th Int Congress on Marine Corrosion and Fouling. Centre de Recherches et d'Etudes Oceanogr, Boulogne, France. pp 137–144

    Google Scholar 

  22. Dexter SC (1979) Influence of substratum critical surface tension on bacterial adhesion: in situ studies. J Coll Inter Sci 70:346–354

    Google Scholar 

  23. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    CAS  Google Scholar 

  24. Duddridge JE, Kent CA, Laws JF (1982) Effect of surface shear stress on the attachment of Pseudomonas fluorescens to stainless steel under defined flow conditions. Biotech Bioeng 24:153–164

    Google Scholar 

  25. Evans LV, Clarkson N (1993) Antifouling strategies in the marine environment.J Appl Bact (Symposium Suppl) 74:119–124

    Google Scholar 

  26. Fattom A, Shilo M (1984) Hydrophobicity as an adhesion mechanism of benthic cyanobacteria. Appl Environ Microbiol 47:135–143

    Google Scholar 

  27. Fletcher M, Floodgate GD (1973) An electron-microscopic demonstration of an acidic polysaccharide involved in the adhesion of a marine bacterium to solid surfaces. J Gen Microbiol 74: 325–334

    Google Scholar 

  28. Fletcher M, Loeb GI (1979) Influence of substratum characteristics on the attachment of a marine Pseudomonad to solid surfaces. Appl Environ Microbiol 37:67–72

    Google Scholar 

  29. Fletcher M, Marshall KC (1982) Bubble contact angle method for evaluating substratum interfacial characteristics and its relevance to bacterial attachment. Appl Environ Microbiol 44(1):184–192

    Google Scholar 

  30. Fletcher M, Lessmann JM, Loeb GI (1991) Bacterial surface adhesives and biofilm matrix polymers of marine and freshwater bacteria. Biofouling 4:129–140

    Google Scholar 

  31. Ford T, Sacco E, Black J, Kelley T, Goodacre R, Berkeley RCW, Mitchell R (1991) Characterization of exopolymers of aquatic bacteria by pyrolysis-mass spectrometry. Appl Environ Microbiol 57:1595–1601

    Google Scholar 

  32. Fowler HW, McKay AJ (1980) The measurement of microbial adhesion. In: Berkeley RCW, Lynch JM, Melling J, Butler PR, Vincent B (eds) Microbial adhesion to surfaces. Ellis Horwood Ltd, Chichester, pp 143–161

    Google Scholar 

  33. Hoagland KD, Rosowski JD, Gretz MR, Roemer SC (1993) Diatom extracellular polymeric substances: function, fine structure, chemistry, and physiology. J Phycol 29:537–566

    Google Scholar 

  34. Hyde KD, Moss ST, Jones EBG (1989) Attachment studies in marine fungi. Biofouling 1:287–298

    Google Scholar 

  35. Jackson SM, Jones EBG (1991) Interactions within biofilms: disruption of biofilm structure by protozoa. Kieler Meeresforsch Sonderh 8:264–268

    Google Scholar 

  36. Kefford B, Marshall KC (1986) The role of bacterial surface and substratum hydrophobicity in adhesion of Leptospira biflexa serovar patoc 1 to inert surfaces. Microb Ecol 12:315–322

    Google Scholar 

  37. Kjelleberg S, Marshall KC, Hermansson M (1985) Oligotrophic and copiotrophic marine bacteria: observations related to attachment. FEMS Microbiol Ecol 31:89–96

    Google Scholar 

  38. Lappin-Scott HM, Costerton JW (1989) Bacterial biofilms and surface fouling. Biofouling 1:323–342

    Google Scholar 

  39. Lindner E (1992) A low surface energy approach in the control of marine biofouling. Biofouling 6:193–205

    Google Scholar 

  40. Loosdrecht van MCM, Lyklema J, Norde W, Schraa G, Zehnder A (1987) Electrophoretic mobility and hydrophobicity as a measure to predict the initial steps of bacterial adhesion. Appl Environ Microbiol 53:1898–1901

    Google Scholar 

  41. MacRitchie F (1972) The adsorption of proteins at the solid/liquid interface. J Coll Interf Sci 38:484–488

    Google Scholar 

  42. Mian FA, Jarman TR, Righelato RC (1978) Biosynthesis of exopolysaccharides by Pseudomonas aeruginosa. J Bact 134:418–422

    Google Scholar 

  43. Neu TR, Marshall KC (1991) Microbial “footprints”: a new approach to adhesive polymers. Biofouling 3:101–112

    Google Scholar 

  44. Neu TR, Poralla K (1988) An amphiphilic polysaccharide from an adhesive Rhodococcus strain. FEMS Microbiol Lett 49:389–392

    Google Scholar 

  45. Neumann AW, Good RJ (1979) Techniques of measuring contact angles. In: Good RJ, Stromberg RR (eds) Experimental Methods. (Surface and Colloid Science, vol 11) Plenum Press, New York London, pp 31–91

    Google Scholar 

  46. Neumann AW Absolom DR, Francis DW, van, Oss CJ (1980) Conversion tables of contact angles to surface tensions. Separ Purif Meth 9:69–163

    Google Scholar 

  47. Oss van CJ (1991) The forces involved in bioadhesion to flat surfaces and particles: their determination and relative roles. Biofouling 4:25–35

    Google Scholar 

  48. Paul JH, Jeffrey WH (1985) Evidence for separate adhesion mechanisms for hydrophilic and hydrophobic surfaces in Vibrio proteolytica. Appl Environ Microbiol 50:431–437

    Google Scholar 

  49. Pelt van WJ, Weerkamp AH, Uyen MHWJC, Bussher HJ, de Jong HP, Arends J (1985) Adhesion of Streptococcus sanguis CH3 to polymers with different surface free energies. Appl Environ Microbiol 49:1270–1275

    Google Scholar 

  50. Pyne S, Fletcher RL, Jones EBG (1984) Attachment studies on three common fouling diatoms. In Proc 6th Int Congress on Marine Corrosion and Fouling, Athens, Greece, pp 99–112

  51. Quintero EJ, Weiner RM (1995) Evidence for the adhesive function of the exopolysaccharide of Hyphomonas strain MHS-3 in its attachment to surfaces. Appl Environ Microbiol 61:1897–1903

    Google Scholar 

  52. Rabel W (1971) Einige Aspekte der Benetzungstheorie and ihre Anwendung auf die Untersuchung and Veränderung der Oberflächeneigenschaften von Polymeren. Farbe and Lack 77:997–1005

    Google Scholar 

  53. Rittle KH, Helmstetter CE, Meyer AE, Baier RE (1990) Escherischa coli retention on solid surfaces as functions of substratum surface free energy and cell growth phase. Biofouling 2:121–130

    Google Scholar 

  54. Robb ID (1984) Stereo-biochemistry and function of polymers. In: Marshall KC (ed) Microbial Adhesion and Aggregation. (Dahlem Konferenzen) Springer, Berlin, Heidelberg New York, pp 39–49

    Google Scholar 

  55. Rutter PR (1980) The physical chemistry of the adhesion of bacteria and other cells. In: Curtis ASG, Pitts JD (eds) Cell adhesion and mobility. (Brit Soc Cell Biol, 3rd Symp) London, pp 103–135

  56. Shea C, Nunley JW Williamson JC, Smith-Sommerville HE (1991) Comparison of the adhesion properties of Deleya marina and the exopolysaccharide-defective mutant strain DMR. Appl Environ Microbiol 57:3107–3113

    Google Scholar 

  57. Sutherland IW (1977) Bacterial exopolysaccharides: their nature and production. In: Sutherland IW (ed) Surface carbohydrates of the procaryotic cell. Academic Press, London New York San Francisco, pp 27–96

    Google Scholar 

  58. Sutherland IW (1980) Polysaccharides in the adhesion of marine and freshwater bacteria. In: Berkeley RCW, Lynch JM, Melling J, Rutter PR, Vincent B (eds) Microbial Adhesion to Surfaces. Ellis Horwood, Chichester, pp 330–338

    Google Scholar 

  59. Weast RC (ed) (1988) Handbook of chemistry and physics, 1st student ed. CRC Press Inc, Boca Raton

    Google Scholar 

  60. Webster DR, Cooksey KE, Rubin RW (1985) An investigation of the involvement of cytoskeletal structures and secretion of gliding motility of the marine diatom, Amphora coffaeformis. Cell Motility 5:103–122

    Google Scholar 

  61. Wigglesworth-Cooksey B, Cooksey KE (1992) Can diatoms sense surfaces? State of our knowledge. Biofouling 5:227–238

    Google Scholar 

  62. Woods DC, Fletcher RL (1991). Studies on the strength of adhesion of some common marine fouling diatoms. Biofouling 3:287–303

    Google Scholar 

  63. Wu S (1973) Polar and nonpolar interactions in adhesion. J Adhesion 5:39–55

    Google Scholar 

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  1. K. Becker

    Present address: BIOLAB Forschunginstitut, Kieler Str. 51, D-24594, Hohenwestedt, Germany

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  1. Institute of Zoology, University of Kiel, Olshausenstr. 40, D-24098, Kiel, Germany

    K. Becker

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Becker, K. Exopolysaccharide production and attachment strength of bacteria and diatoms on substrates with different surface tensions. Microb Ecol 32, 23–33 (1996). https://doi.org/10.1007/BF00170104

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