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Spectrophotometric determination of mycelial biomass

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Biotechnology Techniques

Abstract

The optical density (450 nm) of samples of homogenized fungal biomass correlated linearly with the dry weight of the biomass in the samples. As shown for broths of the filamentous microfungus Neurospora sitophila, the sensitivity of the technique depended on the extent of fragmentation of fungal hyphae during homogenization: increased fragmentation increased sensitivity. The method applied during all phases of growth, was as accurate as the conventional dry weight technique and permitted rapid and simple measurement of biomass concentration.

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References

  • Chisti, Y. and Moo-Young, M. (1986). Enz. Microbial. Technol., 8, 194–204.

    Google Scholar 

  • Grenade, T. C., Hehmann, M. F. and Artis, W. M. (1985)., Appl. Environ. Microbiol., 49, 101–108.

    Google Scholar 

  • Ingham, E. R. and Klein, D. A. (1982). Appl. Environ. Microbiol., 44, 363–370.

    Google Scholar 

  • Nestaas, E., Wang, D. I. C., Suzuki, H. and Evans, L. B. (1981). Biotechnol. Bioeng., 23, 2815–2824.

    Google Scholar 

  • Perley, C. R., Swartz, J. R. and Cooney, C. L. (1979). Biotechnol. Bioeng., 21, 519–523.

    Google Scholar 

  • Pirt, S. J. (1975). Principles of Microbe and Cell Cultivation, Blackwell, Oxford.

    Google Scholar 

  • Seitz, L. M., Sauer, 0D. B., Burroughs, R., Mohr, A. E. and Hubbard, J. D. (1979). Physiol. Biochem., 69, 1202–1203.

    Google Scholar 

  • Sharma, P. D., Fisher, P. J. and Webster, J. (1977). Trans. Er. Mycol. Soc., 69, 479–483.

    Google Scholar 

  • Trinci, A. P. J. (1974). J. Gen. Microbiol., 81, 225–236.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    U. C. Banerjee, Y. Chisti & M. Moo-Young

Authors
  1. U. C. Banerjee
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  2. Y. Chisti
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  3. M. Moo-Young
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Banerjee, U.C., Chisti, Y. & Moo-Young, M. Spectrophotometric determination of mycelial biomass. Biotechnol Tech 7, 313–316 (1993). https://doi.org/10.1007/BF00150905

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  • DOI: https://doi.org/10.1007/BF00150905

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