Summary
As Phaffia rhodozyma is a Crabtree positive yeast, its cell yield and pigment production are reduced at high sugar concentrations. A method for maintaining low growth medium sugar concentrations is fed-batch culture. Using a mass balance approach and Monod growth kinetics a model is presented which describes the fed-batch culture of Phaffia rhodozyma and enables the calculation of a feed regime to obtain the maximum yield of cells and pigment. Although developed on a glucose medium, the model was also applied successfully to a molasses-based medium.
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Reynders, M.B., Rawlings, D.E. & Harrison, S.T.L. Studies on the growth, modelling and pigment production by the yeast Phaffia rhodozyma during fed-batch cultivation. Biotechnol Lett 18, 649–654 (1996). https://doi.org/10.1007/BF00130759
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DOI: https://doi.org/10.1007/BF00130759