Skip to main content
SpringerLink
Log in

Plant regeneration from embryogenic callus initiated from immature inflorescences of several high-tannin sorghums

  • Articles
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

A procedure was established for the induction of regenerable calli from immature inflorescence segments of high-tannin cultivars of sorghum (Sorghum bicolor (L.) Moench). Murashige & Skoog's medium with several components altered was utilized for inducing, maintaining, and regenerating the cultures. Embryogenic calli formed at a frequency of 8–70% depending on the genotype. During a ten-month period, 3600 plants were regenerated from eight genotypes tested. Among the developmental stages of immature inflorescence tested (from differentiation of secondary branch primordia to floret formation) no critical differences were found in potential for callusing, embryogenesis or regeneration. Genotypic differences were observed in pigment production, embryogenic callus formation, shoot differentiation, and in maintenance of regeneration capacity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

2,4-D:

dichlorophenoxyacetic acid

References

  1. Butler LG, Rogler JC, Mehansho H, Carlson DM (1986) Dietary effects of tannins. In: Plant Flavonoids in Biology and Medicine: Biochemical, Pharmacological, and Structure-Activity Relationships (pp 141–157) Alan R Liss Inc, New York

    Google Scholar 

  2. Maddock SE, Lancaster VA, Risiott R, Franklin J (1983) Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum). J Exp Bot 34: 915–926

    Google Scholar 

  3. Shi LH, Zhang YB, Zhou MJ (1985) In vitro response of immature inflorescence of different ploidy rice. Kexue Tongbao 30: 221–224

    Google Scholar 

  4. Suprasama P, Rao KV, Reddy GM (1986) Plantlet regeneration from glume calli of maize (Zea mays L.). Theor Appl Genet 72: 120–122

    Google Scholar 

  5. Thomas MR, Scott KJ (1985) Plant regeneration by somatic embryogenesis from callus initiated from immature embryos and immature inflorescences of Hordeum vulgare. J Plant Physiol 121: 159–169

    Google Scholar 

  6. Xu ZH, Wang DY, Yang LJ, Wei ZM (1984) Somatic embryogenesis and plant regeneration in cultured immature inflorescences of Setiria italica. Plant Cell Rep 3: 149–150

    Google Scholar 

  7. Krumbiegel-Schroeren G, Finger J, Schroeren V, Binding H (1984) Embryoid formation and plant regeneration from callus of Secale cereale. Z Pflanzenzucht 92: 89–94

    Google Scholar 

  8. Nakamura C, Keller WA (1982) Plant regeneration from inflorescence cultures of hexaploid triticale. Plant Sci Lett 24: 275–280

    Google Scholar 

  9. Conger BV, McDonnell RE (1983) Plant formation from cultured inflorescences of Dactylis glomerate L. Plant Cell tissue Organ Culture 2: 191–197

    Google Scholar 

  10. Wang DY, Vasil IK (1982) Somatic embryogenesis and plant regeneration from inflorescence segments of Pennisetum purpureum Schum. Plant Sci Lett 25: 147–154

    Google Scholar 

  11. Boyes CJ, Vasil IK (1984) Plant regeneration by somatic embryogenesis from cultured young inflorescences of Sorghum arundinaceum (Desv.) Stapf. var. sudanense (sudan grass). Plant Sci Lett 35: 153–157

    Google Scholar 

  12. Ahn BJ, Huang FH, King JW (1987) Regeneration of bermuda grass cultivars and evidence of somatic embryogenesis. Crop Sci 27: 594–597

    Google Scholar 

  13. Songated DD, Chen CH, Boe AA (1986) Plant regeneration in callus cultures derived from young inflorescences of little bluestem. Crop Sci 26: 827–829

    Google Scholar 

  14. Brettell RIS, Wernicke W, Thomas E (1980) Embryogenesis from cultured immature inflorescences of Sorghum bicolor. Protoplasma 104: 141–148

    Google Scholar 

  15. Price ML, Van Scoyoc S, Butler LG (1978) A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. J Agric Food Chem 26: 1214–1218

    Google Scholar 

  16. Cai T, Daly B, Butler LG (1987) Callus induction and plant regeneration from shoot portions of mature embryos of high tannin sorghum. Plant Cell Tissue Organ Culture 9: 245–252

    Google Scholar 

  17. House LR (1980) The sorghum plant: growth stages and morphology. In: A Guide to Sorghum Breeding (p 16–31) ICRISAT Patancheru P.O. Andhra Pradesh, India

    Google Scholar 

  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  19. Maddock SE (1985) Cell culture, somatic embryogenesis and plant regeneration in wheat, barley, oats, rye and triticale. In: Bright SWJ, Jones MGK (Eds) Cereal Tissue and Cell Culture (p 131–174) Martinus Nijhoff, The Hague

    Google Scholar 

  20. Tomes DT (1985) Cell culture, somatic embryogenesis and plant regeneration in maize, rice, sorghum and millets. In: Bright SWJ, Jones MGK (Eds) Cereal Tissue and Cell Culture (p 175–203) Martinus Nijhoff, The Hague

    Google Scholar 

  21. Oberthur EE, Nicholson RL, Butler LG (1983) Presence of polyphenolic materials, including condensed tannins, in sorghum callus. J Agric Food Chem 31: 660–662

    Google Scholar 

  22. Springer WD, Green CE, Kohn KA (1979) A histological examination of tissue culture initiation from immature embryos of maize. Protoplasma 101: 269–281

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Purdue University, 47907, West Lafayette, IN, USA

    Tishu Cai & Larry Butler

Authors
  1. Tishu Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Larry Butler
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This is Journal Paper Number 11972 from the Purdue University Agricultural Experiment Station

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cai, T., Butler, L. Plant regeneration from embryogenic callus initiated from immature inflorescences of several high-tannin sorghums. Plant Cell Tiss Organ Cult 20, 101–110 (1990). https://doi.org/10.1007/BF00114707

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00114707

Key words

Search

Navigation