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The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill

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Abstract

Somatic embryos of Cyclamen persicum Mill. could be produced through a callus phase from juvenile explant material including anthers, ovaries and zygotic embryos. The auxin 2,4-D (1.0–1.5 mg l-1) and coconut milk (10% v/v) in MS medium were important factors for the induction of somatic embryogenesis. Somatic embryos germinated into plantlets in MS medium without growth regulators. The plants grew well in the greenhouse and flowered normally. The plants were phenotypically identical to the mother plants with a few exceptions.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-naphthylacetic acid

IAA:

3-indoleacetic acid

BA:

6-benzyladenine

ABA:

abscisic acid

CM:

coconut milk

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Authors and Affiliations

  1. KEMIRA, Espoo Research Centre, P.O. Box 44, SF-02271, Espoo, Finland

    Elina Kiviharju, Ulla Tuominen & Timo Törmälä

Authors
  1. Elina Kiviharju
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  2. Ulla Tuominen
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  3. Timo Törmälä
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Kiviharju, E., Tuominen, U. & Törmälä, T. The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill. Plant Cell Tiss Organ Cult 28, 187–194 (1992). https://doi.org/10.1007/BF00055516

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  • DOI: https://doi.org/10.1007/BF00055516

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