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Isolation of putative defense-related genes from Arabidopsis thaliana and expression in fungal elicitor-treated cells

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Abstract

Numerous Arabidopsis genes have been cloned that correspond to putative pathogen defense-related genes identified in parsley (Petroselinum crispum). Treatment of Arabidopsis cells with fungal elicitor leads to rapid accumulation of the respective mRNAs with time courses comparable to those observed for their counterparts in parsley. Evolutionary sequence conservation of many of these genes in several plant species suggests they code for important plant functions.

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References

  1. Dixon RA, Harrison MJ: Activation, structure, and organization of genes involved in microbial defense in plants. Adv Genet 28: 165–234 (1990).

    Google Scholar 

  2. Bowles DJ: Defense-related proteins in higher plants. Annu Rev Biochem 59: 873–907 (1990).

    Google Scholar 

  3. Hahlbrock K, Scheel D: Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Mol Biol 40: 347–369 (1989).

    Google Scholar 

  4. Somssich IE, Bollmann J, Hahlbrock K, Kombrink E, and Schulz W. Differential early activation of defenserelated genes in elicitor-treated parsley cells. Plant Mol Biol 12: 227–234 (1989).

    Google Scholar 

  5. Schmelzer E, Krüger-Lebus S, Hahlbrock K: Temporal and spatial patterns of gene expression around sites of attempted fungal infection in parsley leaves. Plant Cell 1: 993–1001 (1989).

    Google Scholar 

  6. Murray MG, Thompson WF: Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 8: 6323–6327 (1980).

    Google Scholar 

  7. Lois R, Dietrich A, Hahlbrock K, Schulz W: A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements. EMBO J 8: 1641–1648 (1989).

    Google Scholar 

  8. Somssich IE, Schmelzer E, Bollmann J, Hahlbrock K: Rapid activation by fungal elicitor of genes encoding ‘pathogenesis-related’ proteins in cultured parsley cells. Proc Natl Acad Sci USA 83: 2427–2430 (1986).

    Google Scholar 

  9. Kawalleck P: Pathogenresistenz in Planzen: Identifizierung von Genen, deren Produkte an induzierten Abwehrreaktionen in Petersilie beteiligt sind. Dissertation, University of Cologne, FRG (1991).

    Google Scholar 

  10. Kawalleck P, Plesch G, Hahlbrock K, Somssich IE: Induction by fungal elicitor of S-adenosyl-L-methionine synthetase and S-adenosyl-L-homocysteine hydrolase mRNAs in cultured cells and leaves of Petroselinum crispum. Proc Natl Acad Sci USA 89: 4713–4717 (1992).

    Google Scholar 

  11. Douglas C, Hoffmann H, Schulz W, Hahlbrock K: Structure and elicitor or u.v.-light-stimulated expression of two 4-coumarate: CoA ligase genes in parsley. EMBO J 6: 1189–1195 (1987).

    Google Scholar 

  12. Reimold U, Kroger M, Kreuzaler F, Hahlbrock K: Coding and 3′ non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme. EMBO J 2: 1801–1805 (1983).

    Google Scholar 

  13. Feinbaum RL, Ausubel FM: Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene. Mol Cell Biol 8: 1985–1992 (1988).

    Google Scholar 

  14. Davis KR, Schott H, Dong X, Ausubel FM: Arabidopsis thaliana as a model system for studying plant-pathogen interactions. In: Lugtenberg BJJ (ed) Signal Molecules in Plants and Plant-Microbe Interactions, pp. 99–106. Springer-Verlag, Berlin (1989).

    Google Scholar 

  15. Dangl JL, Lehnacker H, Kiedrowski S, Debener T, Rupprecht C, Arnold M, Somssich IE: Interactions between Arabidopsis thaliana and phytopathogenic Pseudomonas pathovars: a model for the genetics of disease resistance. In: Hauke H, Verma DPS (eds) Current Plant Science and Biotechnology in Agriculture, Vol. 1: Advances in Molecular Genetics of Plant-Microbe Interactions, pp. 84–89. Kluwer Academic Publishers, Dordrecht (1991).

    Google Scholar 

  16. Koch E, Slusarenko A: Arabidopsis is susceptible to infection by a downy mildew fungus. Plant Cell 2: 437–445 (1990).

    Google Scholar 

  17. Roberts E, Kutchan T, Kolattukudy PE: Cloning and sequencing of cDNA for a highly anionic peroxidase from potato and the induction of its mRNA in suberizing potato tubers and tomato fruits. Plant Mol Biol 11: 15–26 (1988).

    Google Scholar 

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Trezzini, G.F., Horrichs, A. & Somssich, I.E. Isolation of putative defense-related genes from Arabidopsis thaliana and expression in fungal elicitor-treated cells. Plant Mol Biol 21, 385–389 (1993). https://doi.org/10.1007/BF00019954

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  • DOI: https://doi.org/10.1007/BF00019954

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