Abstract
A chimeric gene consisting of the 5′ flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days after flowering and obtained a maximum level at 16 days after flowering, the onset of overall protein accumulation. Significant expression of CAT activity in non-seed tissues occurred in some, but not all plants, suggesting possible chromosome position effects on non-seed tissue expression. A positive correlation was observed between expression levels in seeds and gene copy numbers.
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Leisy, D.J., Hnilo, J., Zhao, Y. et al. Expression of a rice glutelin promoter in transgenic tobacco. Plant Mol Biol 14, 41–50 (1990). https://doi.org/10.1007/BF00015653
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DOI: https://doi.org/10.1007/BF00015653