Abstract
Pseudomonas putida is increasingly attracting attention as a bacterial host of reference both for basic and applied research. Over the years, this Gram-negative soil bacterium has been considered a potential agent for environmental bioremediation of industrial wastes and xenobiotic compounds and also a promising colonizer of the rhizosphere. However, the potential biotechnological applications of P. putida were enormously multiplied by the advent of contemporary synthetic biology which, together with the wealth of information provided by systems-level analysis of its genome, transcriptome, proteome, metabolome, and fluxome, enabled the implementation of targeted metabolic engineering approaches. This chapter summarizes the main discoveries within this context that mediated the transition of P. putida from its humble origin in the soil to modern biotechnology setups, where it excels in a number of practical applications for which other traditional microbial cell factories cannot be used (e.g., in hosting harsh oxidative reactions for the production of valuable chemicals).
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Acknowledgment
The author is indebted to Prof. VÃctor de Lorenzo (CNB-CSIC), the enlightening tutelage and continuous support of whom has been instrumental to develop many of the studies on which this chapter is based. The works by the author have been financed by the European Molecular Biology Organization and the Marie SkÅ‚odowska-Curie Actions from the European Commission. There is no conflict of interest related to the contents of this chapter.
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Nikel, P.I. (2016). Systems and Synthetic Biology Approaches for Metabolic Engineering of Pseudomonas putida . In: Castro-Sowinski, S. (eds) Microbial Models: From Environmental to Industrial Sustainability. Microorganisms for Sustainability, vol 1. Springer, Singapore. https://doi.org/10.1007/978-981-10-2555-6_1
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