Abstract
In the last decade, the genes encoding hyperthermophilic proteins and enzymes have been extensively expressed in heterologous organisms such as Escherichia coli, and their good productions have been achieved. However, some difficulties are often encountered when attempting to produce these proteins in the mesophilic hosts. This chapter focuses on the recent efforts made to overcome problems in heterologous production of hyperthermophilic enzymes: (1) successful production of hetero-oligomeric dye-linked l-proline dehydrogenases by use of effective promoters, (2) a typical procedure for the in vitro refolding of inclusion bodies composed of several hyperthermophilic enzymes (malate dehydrogenase, lysine dehydrogenase, and agmatinase), and (3) heat-induced structural conversion of hyperthermophilic glutamate dehydrogenase. This information could be useful in successful production of hyperthermophilic proteins and enzymes.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Abd Rahman RNZA, Fujiwara S, Takagi M, Kanaya S, Imanaka T (1997) Biochem Biophys Res Commun 241:646–652
Adams MWW (1993) Annu Rev Microbiol 47:627–658
Bhuiya MW, Sakuraba H, Ohshima T, Imagawa T, Katunuma N, Tsuge H (2005) J Mol Biol 345:325–337
Diruggiero J, Robb FT (1995) Appl Environ Microbiol 61:159–164
Frew JE, Hill HA (1987) Anal Chem 59:933A–944A
Gill SC, von Hippel PH (1989) Anal Biochem 182:319–326
Glatter O, Kratky O (1982) Small-angle X-ray scattering. Academic, New York
Goda S, Kojima M, Nishikawa Y, Kujo C, Kawakami R, Kuramitsu S, Sakuraba H, Hiragi Y, Ohshima T (2005a) Biochemistry 44:15304–15313
Goda S, Sakuraba H, Kawarabayasi Y, Ohshima T (2005b) Biochim Biophys Acta 1748:110–115
Guinier A, Fournet G (1955) Small-angle scattering of X-rays. Chapman & Hall, New York
Ideno A, Yoshida T, Iida T, Furutani M, Maruyama T (2001) Biochem J 357:465–471
Ideno A, Furutani M, Iwabuchi T, Iida T, Iba Y, Kurosawa Y, Sakuraba H, Ohshima T, Kawarabayashi Y, Maruyama T (2004) Appl Microbiol Biotechnol 64:99–105
Iida T, Furutani M, Nishida F, Maruyama T (1998) Gene 222:249–255
Kajiwara K, Hiragi Y (1996) Structure analysis by small-angle X-ray scattering. Elsevier, Amsterdam, pp 353–404
Kawakami R, Sakuraba H, Ohshima T (2004) Extremophiles 8:99–108
Kawakami R, Sakuraba H, Tsuge H, Goda S, Katunuma N, Ohshima T (2005) FEBS J 272:4044–4054
Kawakami R, Sakuraba H, Goda S, Tsuge H, Ohshima T (2009) Biochim Biophys Acta 1794:1496–1504
Kratky O, Porod G, Kahovec L (1951) Z Elektrochem 55:53–59
Kujo C, Ohshima T (1998) Appl Environ Microbiol 64:2152–2157
Kujo C, Sakuraba H, Nunoura N, Ohshima T (1999) Biochim Biophys Acta 1434:365–371
Ohshima T, Sakuraba H (1986) Biochim Biophys Acta 869:171–177
Ohshima T, Soda K (1989) Trends Biotechnol 7:210–214
Ohshima T, Soda K (2000) Stereoselective biocatalysis: amino acid dehydrogenases and their applications. In: Patel RN (ed) Stereoselective biocatalysis. Marcel Dekker Inc, New York, pp 877–902
Rehaber V, Jaenicke R (1992) J Biol Chem 267:10999–11006
Sakuraba H, Takamatsu Y, Satomura T, Kawakami R, Ohshima T (2001) Appl Environ Microbiol 67:1470–1475
Sakuraba H, Yokono K, Yoneda K, Watanabe A, Asada Y, Satomura T, Yabutani T, Motonaka J, Ohshima T (2010) Arch Biochem Biophys 502:81–88
Satomura T, Kawakami R, Sakuraba H, Ohshima T (2002) J Biol Chem 277:12861–12867
Satomura T, Kawakami R, Sakuraba H, Ohshima T (2008) J Biosci Bioeng 106:16–21
Satomura T, Sakuraba H, Hara Y, Ohshima T (2010) Acta Crystallogr Sect F 66:1508–1510
Satomura T, Zhang XD, Hara Y, Doi K, Sakuraba H, Ohshima T (2011) Appl Microbiol Biotechnol 89:1075–1082
Schroder I, Vadas A, Johnson E, Lim S, Monbouquette HG (2004) J Bacteriol 186:7680–7689
Schultes V, Jaenicke R (1991) FEBS Lett 290:235–238
Siddiqui MA, Fujiwara S, Takagi M, Imanaka T (1998) FEBS Lett 434:372–376
Stetter KO, Fiala G, Huber G, Huber R, Segerer A (1990) FEMS Microbiol Lett 75:117–124
Svergun DI (1999) Biophys J 76:2879–2886
Tani Y, Tanaka K, Yabutani T, Mishima Y, Sakuraba H, Ohshima T, Motonaka J (2008) Anal Chim Acta 619:215–220
Tani Y, Itoyama Y, Nishi K, Wada C, Shoda Y, Satomura T, Sakuraba H, Ohshima T, Hayashi Y, Yabutani T, Motonaka J (2009) Anal Sci 25:919–923
Tsuge H, Kawakami R, Sakuraba H, Ago H, Miyano M, Aki K, Katunuma N, Ohshima T (2005) J Biol Chem 280:31045–31049
Vieille C, Zeikus GJ (2001) Microbiol Mol Biol Rev 65:1–43
Woese CR, Kandler O, Wheelis ML (1990) Proc Natl Acad Sci USA 87:4576–4579
Yoneda K, Fukuda J, Sakuraba H, Ohshima T (2010) J Biol Chem 285:8444–8453
Zheng H, Lin L, Okezaki Y, Kawakami R, Sakuraba H, Ohshima T, Takagi K, Suye S (2010) Beilstein J Nanotech 1:135–141
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Sakuraba, H., Ohshima, T. (2013). Heterologous Production of Thermostable Proteins and Enzymes. In: Satyanarayana, T., Littlechild, J., Kawarabayasi, Y. (eds) Thermophilic Microbes in Environmental and Industrial Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5899-5_15
Download citation
DOI: https://doi.org/10.1007/978-94-007-5899-5_15
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-5898-8
Online ISBN: 978-94-007-5899-5
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)